Publication

A minimally invasive tool to study immune response and skin barrier in children with atopic dermatitis

Hulshof, L., Hack, D. P., Hasnoe, Q. C. J., Dontje, B., Jakasa, I., Riethmüller, C., McLean, W. H. I., Van Aalderen, W. M. C., van't Land, B., Kezic, S., Sprikkelman, A. & Middelkamp-Hup, M. A., Mar-2019, In : Br J Dermatol. 180, 3, p. 621-630 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Hulshof, L., Hack, D. P., Hasnoe, Q. C. J., Dontje, B., Jakasa, I., Riethmüller, C., ... Middelkamp-Hup, M. A. (2019). A minimally invasive tool to study immune response and skin barrier in children with atopic dermatitis. Br J Dermatol, 180(3), 621-630. https://doi.org/10.1111/bjd.16994

Author

Hulshof, Lies ; Hack, D.P. ; Hasnoe, Q.C.J. ; Dontje, B. ; Jakasa, I. ; Riethmüller, C. ; McLean, W.H.I. ; Van Aalderen, W.M.C. ; van't Land, B. ; Kezic, S. ; Sprikkelman, Alida ; Middelkamp-Hup, Maritza A. / A minimally invasive tool to study immune response and skin barrier in children with atopic dermatitis. In: Br J Dermatol. 2019 ; Vol. 180, No. 3. pp. 621-630.

Harvard

Hulshof, L, Hack, DP, Hasnoe, QCJ, Dontje, B, Jakasa, I, Riethmüller, C, McLean, WHI, Van Aalderen, WMC, van't Land, B, Kezic, S, Sprikkelman, A & Middelkamp-Hup, MA 2019, 'A minimally invasive tool to study immune response and skin barrier in children with atopic dermatitis', Br J Dermatol, vol. 180, no. 3, pp. 621-630. https://doi.org/10.1111/bjd.16994

Standard

A minimally invasive tool to study immune response and skin barrier in children with atopic dermatitis. / Hulshof, Lies; Hack, D.P.; Hasnoe, Q.C.J.; Dontje, B.; Jakasa, I.; Riethmüller, C.; McLean, W.H.I.; Van Aalderen, W.M.C.; van't Land, B.; Kezic, S.; Sprikkelman, Alida; Middelkamp-Hup, Maritza A.

In: Br J Dermatol, Vol. 180, No. 3, 03.2019, p. 621-630.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Hulshof L, Hack DP, Hasnoe QCJ, Dontje B, Jakasa I, Riethmüller C et al. A minimally invasive tool to study immune response and skin barrier in children with atopic dermatitis. Br J Dermatol. 2019 Mar;180(3):621-630. https://doi.org/10.1111/bjd.16994


BibTeX

@article{be12436a604941b49052fd502c047c7c,
title = "A minimally invasive tool to study immune response and skin barrier in children with atopic dermatitis",
abstract = "Background Atopic dermatitis (AD) affects children of all skin types. Most research has focused on light skin types. Studies investigating biomarkers in people with AD with dark skin types are lacking. Objectives To explore skin barrier and immune response biomarkers in stratum corneum (SC) tape strips from children with AD with different skin types. Methods Tape strips were collected from lesional and nonlesional forearm skin of 53 children with AD and 50 controls. We analysed 28 immunomodulatory mediators, and natural moisturizing factors (NMF) and corneocyte morphology. Results Interleukin (IL)‐1β, IL‐18, C‐X‐C motif chemokine (CXCL) 8 (CXCL8), C‐C motif chemokine ligand (CCL) 22 (CCL22), CCL17, CXCL10 and CCL2 were significantly higher (P < 0·05) in lesional AD skin compared with nonlesional AD skin; the opposite trend was seen for IL‐1α. CXCL8, CCL2 and CCL17 showed an association with objective SCORing Atopic Dermatitis score. NMF levels showed a gradual decrease from healthy skin to nonlesional and lesional AD skin. This gradual decreasing pattern was observed in skin type II but not in skin type VI. Skin type VI showed higher NMF levels in both nonlesional and lesional AD skin than skin type II. Corneocyte morphology was significantly different in lesional AD skin compared with nonlesional AD and healthy skin. Conclusions Minimally invasive tape‐stripping is suitable for the determination of many inflammatory mediators and skin barrier biomarkers in children with AD. This study shows differences between children with AD with skin type II and skin type VI in NMF levels, suggesting that some aspects of pathophysiological mechanisms may differ in AD children with light versus dark skin types.",
author = "Lies Hulshof and D.P. Hack and Q.C.J. Hasnoe and B. Dontje and I. Jakasa and C. Riethm{\"u}ller and W.H.I. McLean and {Van Aalderen}, W.M.C. and {van't Land}, B. and S. Kezic and Alida Sprikkelman and Middelkamp-Hup, {Maritza A.}",
year = "2019",
month = "3",
doi = "10.1111/bjd.16994",
language = "English",
volume = "180",
pages = "621--630",
journal = "BRITISH JOURNAL OF DERMATOLOGY",
issn = "0007-0963",
publisher = "Wiley",
number = "3",

}

RIS

TY - JOUR

T1 - A minimally invasive tool to study immune response and skin barrier in children with atopic dermatitis

AU - Hulshof, Lies

AU - Hack, D.P.

AU - Hasnoe, Q.C.J.

AU - Dontje, B.

AU - Jakasa, I.

AU - Riethmüller, C.

AU - McLean, W.H.I.

AU - Van Aalderen, W.M.C.

AU - van't Land, B.

AU - Kezic, S.

AU - Sprikkelman, Alida

AU - Middelkamp-Hup, Maritza A.

PY - 2019/3

Y1 - 2019/3

N2 - Background Atopic dermatitis (AD) affects children of all skin types. Most research has focused on light skin types. Studies investigating biomarkers in people with AD with dark skin types are lacking. Objectives To explore skin barrier and immune response biomarkers in stratum corneum (SC) tape strips from children with AD with different skin types. Methods Tape strips were collected from lesional and nonlesional forearm skin of 53 children with AD and 50 controls. We analysed 28 immunomodulatory mediators, and natural moisturizing factors (NMF) and corneocyte morphology. Results Interleukin (IL)‐1β, IL‐18, C‐X‐C motif chemokine (CXCL) 8 (CXCL8), C‐C motif chemokine ligand (CCL) 22 (CCL22), CCL17, CXCL10 and CCL2 were significantly higher (P < 0·05) in lesional AD skin compared with nonlesional AD skin; the opposite trend was seen for IL‐1α. CXCL8, CCL2 and CCL17 showed an association with objective SCORing Atopic Dermatitis score. NMF levels showed a gradual decrease from healthy skin to nonlesional and lesional AD skin. This gradual decreasing pattern was observed in skin type II but not in skin type VI. Skin type VI showed higher NMF levels in both nonlesional and lesional AD skin than skin type II. Corneocyte morphology was significantly different in lesional AD skin compared with nonlesional AD and healthy skin. Conclusions Minimally invasive tape‐stripping is suitable for the determination of many inflammatory mediators and skin barrier biomarkers in children with AD. This study shows differences between children with AD with skin type II and skin type VI in NMF levels, suggesting that some aspects of pathophysiological mechanisms may differ in AD children with light versus dark skin types.

AB - Background Atopic dermatitis (AD) affects children of all skin types. Most research has focused on light skin types. Studies investigating biomarkers in people with AD with dark skin types are lacking. Objectives To explore skin barrier and immune response biomarkers in stratum corneum (SC) tape strips from children with AD with different skin types. Methods Tape strips were collected from lesional and nonlesional forearm skin of 53 children with AD and 50 controls. We analysed 28 immunomodulatory mediators, and natural moisturizing factors (NMF) and corneocyte morphology. Results Interleukin (IL)‐1β, IL‐18, C‐X‐C motif chemokine (CXCL) 8 (CXCL8), C‐C motif chemokine ligand (CCL) 22 (CCL22), CCL17, CXCL10 and CCL2 were significantly higher (P < 0·05) in lesional AD skin compared with nonlesional AD skin; the opposite trend was seen for IL‐1α. CXCL8, CCL2 and CCL17 showed an association with objective SCORing Atopic Dermatitis score. NMF levels showed a gradual decrease from healthy skin to nonlesional and lesional AD skin. This gradual decreasing pattern was observed in skin type II but not in skin type VI. Skin type VI showed higher NMF levels in both nonlesional and lesional AD skin than skin type II. Corneocyte morphology was significantly different in lesional AD skin compared with nonlesional AD and healthy skin. Conclusions Minimally invasive tape‐stripping is suitable for the determination of many inflammatory mediators and skin barrier biomarkers in children with AD. This study shows differences between children with AD with skin type II and skin type VI in NMF levels, suggesting that some aspects of pathophysiological mechanisms may differ in AD children with light versus dark skin types.

U2 - 10.1111/bjd.16994

DO - 10.1111/bjd.16994

M3 - Article

VL - 180

SP - 621

EP - 630

JO - BRITISH JOURNAL OF DERMATOLOGY

JF - BRITISH JOURNAL OF DERMATOLOGY

SN - 0007-0963

IS - 3

ER -

ID: 76397585