A Hexose Transporter Homologue Controls Glucose Repression in the Methylotrophic Yeast Hansenula polymorphaStasyk, O. V., Stasyk, O. G., Komduur, J., Veenhuis, M., Cregg, J. M. & Sibirny, A. A., 27-Feb-2004, In : The Journal of Biological Chemistry. 279, 9, p. 8116 - 8125 10 p.
Research output: Contribution to journal › Article › Academic › peer-review
Peroxisome biogenesis and synthesis of peroxisomal enzymes in the methylotrophic yeast Hansenula polymorpha are under the strict control of glucose repression. We identified an H. polymorpha glucose catabolite repression gene (HpGCR1) that encodes a hexose transporter homologue. Deficiency in GCR1 leads to a pleiotropic phenotype that includes the constitutive presence of peroxisomes and peroxisomal enzymes in glucose-grown cells. Glucose transport and repression defects in a UV-induced gcr1-2 mutant were found to result from a missense point mutation that substitutes a serine residue (Ser85) with a phenylalanine in the second predicted transmembrane segment of the Gcr1 protein. In addition to glucose, mannose and trehalose fail to repress the peroxisomal enzyme, alcohol oxidase in gcr1-2 cells. A mutant deleted for the GCR1 gene was additionally deficient in fructose repression. Ethanol, sucrose, and maltose continue to repress peroxisomes and peroxisomal enzymes normally and therefore, appear to have GCR1-independent repression mechanisms in H. polymorpha. Among proteins of the hexose transporter family of baker’s yeast, Saccharomyces cerevisiae, the amino acid sequence of the H. polymorpha Gcr1 protein shares the highest similarity with a core region of Snf3p, a putative high affinity glucose sensor. Certain features of the phenotype exhibited by gcr1 mutants suggest a regulatory role for Gcr1p in a repression pathway, along with involvement in hexose transport.
|Pages (from-to)||8116 - 8125|
|Number of pages||10|
|Journal||The Journal of Biological Chemistry|
|Publication status||Published - 27-Feb-2004|
- CARBON CATABOLITE REPRESSION, SACCHAROMYCES-CEREVISIAE, ALCOHOL OXIDASE, PEROXISOME BIOGENESIS, METHANOL UTILIZATION, REGULATORY MUTANT, GENE ENCODES, PROTEIN, DEGRADATION, CELLS
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