Publication

A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage

Chang, M., Bellaoui, M., Boone, C. & Brown, GW., 24-Dec-2002, In : Proceedings of the National Academy of Sciences of the United States of America. 99, 26, p. 16934-16939 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Chang, M., Bellaoui, M., Boone, C., & Brown, GW. (2002). A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage. Proceedings of the National Academy of Sciences of the United States of America, 99(26), 16934-16939. https://doi.org/10.1073/pnas.262669299

Author

Chang, M ; Bellaoui, M ; Boone, C ; Brown, GW. / A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 26. pp. 16934-16939.

Harvard

Chang, M, Bellaoui, M, Boone, C & Brown, GW 2002, 'A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage' Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 26, pp. 16934-16939. https://doi.org/10.1073/pnas.262669299

Standard

A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage. / Chang, M; Bellaoui, M; Boone, C; Brown, GW.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 26, 24.12.2002, p. 16934-16939.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Chang M, Bellaoui M, Boone C, Brown GW. A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage. Proceedings of the National Academy of Sciences of the United States of America. 2002 Dec 24;99(26):16934-16939. https://doi.org/10.1073/pnas.262669299


BibTeX

@article{aa87ad1bbae146f68852b59defc20c73,
title = "A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage",
abstract = "We performed a systematic screen of the set of approximate to5,000 viable Saccharomyces cerevisiae haploid gene deletion mutants and have identified 103 genes whose deletion causes sensitivity to the DNA-damaging agent methyl methanesulfonate (MMS). In total, 40 previously uncharacterized alkylation damage response genes were identified. Comparison with the set of genes known to be transcriptionally induced in response to MMS revealed surprisingly little overlap with those required for MMS resistance, indicating that transcriptional regulation plays little, if any, role in the response to MMS damage. Clustering of the MMS response genes on the basis of their cross-sensitivities to hydroxyurea, UV radiation, and ionizing radiation revealed a DNA damage core of genes required for responses to a broad range of DNA-damaging agents. Of particular significance, we identified a subset of genes that show a specific MMS response, displaying defects in S phase progression only in the presence of MMS. These genes may promote replication fork stability or processivity during encounters between replication forks and DNA damage.",
keywords = "ASSEMBLY FACTOR-I, YEAST SACCHAROMYCES-CEREVISIAE, SISTER-CHROMATID COHESION, BASE EXCISION-REPAIR, DOUBLE-STRAND BREAKS, ESCHERICHIA-COLI, HOMOLOGOUS RECOMBINATION, POSTREPLICATION REPAIR, MITOTIC RECOMBINATION, RADIATION SENSITIVITY",
author = "M Chang and M Bellaoui and C Boone and GW Brown",
year = "2002",
month = "12",
day = "24",
doi = "10.1073/pnas.262669299",
language = "English",
volume = "99",
pages = "16934--16939",
journal = "Proceedings of the National Academy of Science of the United States of America",
issn = "0027-8424",
publisher = "NATL ACAD SCIENCES",
number = "26",

}

RIS

TY - JOUR

T1 - A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage

AU - Chang, M

AU - Bellaoui, M

AU - Boone, C

AU - Brown, GW

PY - 2002/12/24

Y1 - 2002/12/24

N2 - We performed a systematic screen of the set of approximate to5,000 viable Saccharomyces cerevisiae haploid gene deletion mutants and have identified 103 genes whose deletion causes sensitivity to the DNA-damaging agent methyl methanesulfonate (MMS). In total, 40 previously uncharacterized alkylation damage response genes were identified. Comparison with the set of genes known to be transcriptionally induced in response to MMS revealed surprisingly little overlap with those required for MMS resistance, indicating that transcriptional regulation plays little, if any, role in the response to MMS damage. Clustering of the MMS response genes on the basis of their cross-sensitivities to hydroxyurea, UV radiation, and ionizing radiation revealed a DNA damage core of genes required for responses to a broad range of DNA-damaging agents. Of particular significance, we identified a subset of genes that show a specific MMS response, displaying defects in S phase progression only in the presence of MMS. These genes may promote replication fork stability or processivity during encounters between replication forks and DNA damage.

AB - We performed a systematic screen of the set of approximate to5,000 viable Saccharomyces cerevisiae haploid gene deletion mutants and have identified 103 genes whose deletion causes sensitivity to the DNA-damaging agent methyl methanesulfonate (MMS). In total, 40 previously uncharacterized alkylation damage response genes were identified. Comparison with the set of genes known to be transcriptionally induced in response to MMS revealed surprisingly little overlap with those required for MMS resistance, indicating that transcriptional regulation plays little, if any, role in the response to MMS damage. Clustering of the MMS response genes on the basis of their cross-sensitivities to hydroxyurea, UV radiation, and ionizing radiation revealed a DNA damage core of genes required for responses to a broad range of DNA-damaging agents. Of particular significance, we identified a subset of genes that show a specific MMS response, displaying defects in S phase progression only in the presence of MMS. These genes may promote replication fork stability or processivity during encounters between replication forks and DNA damage.

KW - ASSEMBLY FACTOR-I

KW - YEAST SACCHAROMYCES-CEREVISIAE

KW - SISTER-CHROMATID COHESION

KW - BASE EXCISION-REPAIR

KW - DOUBLE-STRAND BREAKS

KW - ESCHERICHIA-COLI

KW - HOMOLOGOUS RECOMBINATION

KW - POSTREPLICATION REPAIR

KW - MITOTIC RECOMBINATION

KW - RADIATION SENSITIVITY

U2 - 10.1073/pnas.262669299

DO - 10.1073/pnas.262669299

M3 - Article

VL - 99

SP - 16934

EP - 16939

JO - Proceedings of the National Academy of Science of the United States of America

JF - Proceedings of the National Academy of Science of the United States of America

SN - 0027-8424

IS - 26

ER -

ID: 14317300