Publication

A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage

Chang, M., Bellaoui, M., Boone, C. & Brown, GW., 24-Dec-2002, In : Proceedings of the National Academy of Sciences of the United States of America. 99, 26, p. 16934-16939 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

We performed a systematic screen of the set of approximate to5,000 viable Saccharomyces cerevisiae haploid gene deletion mutants and have identified 103 genes whose deletion causes sensitivity to the DNA-damaging agent methyl methanesulfonate (MMS). In total, 40 previously uncharacterized alkylation damage response genes were identified. Comparison with the set of genes known to be transcriptionally induced in response to MMS revealed surprisingly little overlap with those required for MMS resistance, indicating that transcriptional regulation plays little, if any, role in the response to MMS damage. Clustering of the MMS response genes on the basis of their cross-sensitivities to hydroxyurea, UV radiation, and ionizing radiation revealed a DNA damage core of genes required for responses to a broad range of DNA-damaging agents. Of particular significance, we identified a subset of genes that show a specific MMS response, displaying defects in S phase progression only in the presence of MMS. These genes may promote replication fork stability or processivity during encounters between replication forks and DNA damage.

Original languageEnglish
Pages (from-to)16934-16939
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number26
Publication statusPublished - 24-Dec-2002
Externally publishedYes

    Keywords

  • ASSEMBLY FACTOR-I, YEAST SACCHAROMYCES-CEREVISIAE, SISTER-CHROMATID COHESION, BASE EXCISION-REPAIR, DOUBLE-STRAND BREAKS, ESCHERICHIA-COLI, HOMOLOGOUS RECOMBINATION, POSTREPLICATION REPAIR, MITOTIC RECOMBINATION, RADIATION SENSITIVITY

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