Publication

A genetically engineered protein domain binding to bacterial murein, archaeal pseudomurein, and fungal chitin cell wall material

Visweswaran, G. R. R., Dijkstra, B. W. & Kok, J., Nov-2012, In : Applied Microbiology and Biotechnology. 96, 3, p. 729-737 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

  • 2012ApplMicrobiolBiotechVisweswaran.pdf

    Final publisher's version, 476 KB, PDF-document

    Request copy

DOI

  • Ganesh Ram R. Visweswaran
  • Bauke W. Dijkstra
  • Jan Kok

The major murein and pseudomurein cell wall-binding domains, i.e., the Lysin Motif (LysM) (Pfam PF01476) and pseudomurein cell wall-binding (PMB) (Pfam PF09373) motif, respectively, were genetically fused. The fusion protein is capable of binding to both murein- and pseudomurein-containing cell walls. In addition, it also binds to chitin, the major polymer of fungal cell walls. Binding is influenced by pH and occurs at a pH close to the pI of the binding protein. Functional studies on truncated versions of the fusion protein revealed that murein and chitin binding is provided by the LysM domain, while binding to pseudomurein is achieved through the PMB domain.

Original languageEnglish
Pages (from-to)729-737
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume96
Issue number3
Publication statusPublished - Nov-2012

    Keywords

  • Murein, Pseudomurein, Chitin, Domain, SURFACE DISPLAY SYSTEM, LACTIC-ACID BACTERIA, LACTOCOCCUS-LACTIS, LYSM DOMAINS, PEPTIDOGLYCAN HYDROLASE, METHANOBACTERIUM-THERMOAUTOTROPHICUM, HETEROLOGOUS PROTEINS, ESCHERICHIA-COLI, GEM PARTICLES, LYTIC ENZYME

View graph of relations

ID: 5690255