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A comparative study of peroxisomal structures in Hansenula polymorpha pex mutants

Koek, A., Komori, M., Veenhuis, M. & van der Klei, I. J., Oct-2007, In : Fems Yeast Research. 7, 7, p. 1126-1133 8 p.

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In a recent study, we performed a systematic genome analysis for the conservation of genes involved in peroxisome biogenesis (PEX genes) in various fungi. We have now performed a systematic study of the morphology of peroxisome remnants ('ghosts') in Hansenula polymorpha pex mutants (pex1-pex20) and the level of peroxins and matrix proteins in these strains. To this end, all available H. polymorpha pex strains were grown under identical cultivation conditions in glucose-limited chemostat cultures and analyzed in detail. The H. polymorpha pex mutants could be categorized into four distinct groups, namely pex mutants containing: (1) virtually normal peroxisomal structures (pex7, pex17, pex20); (2) small peroxisomal membrane structures with a distinct lumen (pex2, pex4, pex5, pex10, pex12, pex14); (3) multilayered membrane structures lacking apparent matrix protein content (pex1, pex6, pex8, pex13); and (4) no peroxisomal structures (pex3, pex19).

Original languageEnglish
Pages (from-to)1126-1133
Number of pages8
JournalFems Yeast Research
Volume7
Issue number7
Publication statusPublished - Oct-2007
Event4th Hansenula Polymorpha Worldwide Network Conference (HPWN) - , Netherlands
Duration: 3-Sep-20065-Sep-2006

Event

4th Hansenula Polymorpha Worldwide Network Conference (HPWN)

03/09/200605/09/2006

Netherlands

Event: Other

    Keywords

  • peroxisome remnants, yeast, peroxisome-deficient mutants, ultrastructure, SACCHAROMYCES-CEREVISIAE, PROTEIN IMPORT, BIOGENESIS FACTORS, PICHIA-PASTORIS, RECEPTOR PEX5P, MATRIX PROTEIN, GENE ENCODES, MEMBRANE, YEAST, MACHINERY

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