A comparative study of peroxisomal structures in Hansenula polymorpha pex mutantsKoek, A., Komori, M., Veenhuis, M. & van der Klei, I. J., Oct-2007, In : Fems Yeast Research. 7, 7, p. 1126-1133 8 p.
Research output: Contribution to journal › Article › Academic › peer-review
In a recent study, we performed a systematic genome analysis for the conservation of genes involved in peroxisome biogenesis (PEX genes) in various fungi. We have now performed a systematic study of the morphology of peroxisome remnants ('ghosts') in Hansenula polymorpha pex mutants (pex1-pex20) and the level of peroxins and matrix proteins in these strains. To this end, all available H. polymorpha pex strains were grown under identical cultivation conditions in glucose-limited chemostat cultures and analyzed in detail. The H. polymorpha pex mutants could be categorized into four distinct groups, namely pex mutants containing: (1) virtually normal peroxisomal structures (pex7, pex17, pex20); (2) small peroxisomal membrane structures with a distinct lumen (pex2, pex4, pex5, pex10, pex12, pex14); (3) multilayered membrane structures lacking apparent matrix protein content (pex1, pex6, pex8, pex13); and (4) no peroxisomal structures (pex3, pex19).
|Number of pages||8|
|Journal||Fems Yeast Research|
|Publication status||Published - Oct-2007|
|Event||4th Hansenula Polymorpha Worldwide Network Conference (HPWN) - , Netherlands|
Duration: 3-Sep-2006 → 5-Sep-2006
4th Hansenula Polymorpha Worldwide Network Conference (HPWN)
03/09/2006 → 05/09/2006Netherlands
- peroxisome remnants, yeast, peroxisome-deficient mutants, ultrastructure, SACCHAROMYCES-CEREVISIAE, PROTEIN IMPORT, BIOGENESIS FACTORS, PICHIA-PASTORIS, RECEPTOR PEX5P, MATRIX PROTEIN, GENE ENCODES, MEMBRANE, YEAST, MACHINERY