Publication

A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis

Essers, P. B., Pereboom, T. C., Goos, Y. J., Paridaen, J. T. & Macinnes, A. W., 15-Jan-2014, In : Developmental Biology. 385, 2, p. 304-315 12 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Essers, P. B., Pereboom, T. C., Goos, Y. J., Paridaen, J. T., & Macinnes, A. W. (2014). A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis. Developmental Biology, 385(2), 304-315. https://doi.org/10.1016/j.ydbio.2013.10.029

Author

Essers, Paul B ; Pereboom, Tamara C ; Goos, Yvonne J ; Paridaen, Judith T ; Macinnes, Alyson W. / A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis. In: Developmental Biology. 2014 ; Vol. 385, No. 2. pp. 304-315.

Harvard

Essers, PB, Pereboom, TC, Goos, YJ, Paridaen, JT & Macinnes, AW 2014, 'A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis', Developmental Biology, vol. 385, no. 2, pp. 304-315. https://doi.org/10.1016/j.ydbio.2013.10.029

Standard

A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis. / Essers, Paul B; Pereboom, Tamara C; Goos, Yvonne J; Paridaen, Judith T; Macinnes, Alyson W.

In: Developmental Biology, Vol. 385, No. 2, 15.01.2014, p. 304-315.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Essers PB, Pereboom TC, Goos YJ, Paridaen JT, Macinnes AW. A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis. Developmental Biology. 2014 Jan 15;385(2):304-315. https://doi.org/10.1016/j.ydbio.2013.10.029


BibTeX

@article{5a1496bf656e49319a922a64de6e31db,
title = "A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis",
abstract = "Nucleostemin (NS) is an essential protein for the growth and viability of developmental stem cells. Its functions are multi-faceted, including important roles in ribosome biogenesis and in the p53-induced apoptosis pathway. While NS has been well studied, the functions of its family members GNL2 and GNL3-like (GNL3L) remain relatively obscure despite a high degree of sequence and domain homology. Here, we use zebrafish lines carrying mutations in the ns family to compare and contrast their functions in vertebrates. We find the loss of zebrafish ns or gnl2 has a major impact on 60S large ribosomal subunit formation and/or function due to cleavage impairments at distinct sites of pre-rRNA transcript. In both cases this leads to a reduction of total protein synthesis. In contrast, gnl3l loss shows relatively minor rRNA processing delays that ultimately have no appreciable effects on ribosome biogenesis or protein synthesis. However, the loss of gnl3l still results in p53 stabilization, apoptosis, and lethality similarly to ns and gnl2 loss. The depletion of p53 in all three of the mutants led to partial rescues of the morphological phenotypes and surprisingly, a rescue of the 60S subunit collapse in the ns mutants. We show that this rescue is due to an unexpected effect of p53 loss that even in wild type embryos results in an increase of 60S subunits. Our study presents an in-depth description of the mechanisms through which ns and gnl2 function in vertebrate ribosome biogenesis and shows that despite the high degree of sequence and domain homology, gnl3l has critical functions in development that are unrelated to the ribosome.",
keywords = "Animals, Base Sequence, DNA Primers, Genes, Lethal, Genes, p53, Nuclear Proteins, Polymerase Chain Reaction, RNA, Ribosomal, Ribosomes, Zebrafish, Zebrafish Proteins, Comparative Study, Journal Article",
author = "Essers, {Paul B} and Pereboom, {Tamara C} and Goos, {Yvonne J} and Paridaen, {Judith T} and Macinnes, {Alyson W}",
note = "{\circledC} 2013 Published by Elsevier Inc.",
year = "2014",
month = "1",
day = "15",
doi = "10.1016/j.ydbio.2013.10.029",
language = "English",
volume = "385",
pages = "304--315",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "ACADEMIC PRESS INC ELSEVIER SCIENCE",
number = "2",

}

RIS

TY - JOUR

T1 - A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis

AU - Essers, Paul B

AU - Pereboom, Tamara C

AU - Goos, Yvonne J

AU - Paridaen, Judith T

AU - Macinnes, Alyson W

N1 - © 2013 Published by Elsevier Inc.

PY - 2014/1/15

Y1 - 2014/1/15

N2 - Nucleostemin (NS) is an essential protein for the growth and viability of developmental stem cells. Its functions are multi-faceted, including important roles in ribosome biogenesis and in the p53-induced apoptosis pathway. While NS has been well studied, the functions of its family members GNL2 and GNL3-like (GNL3L) remain relatively obscure despite a high degree of sequence and domain homology. Here, we use zebrafish lines carrying mutations in the ns family to compare and contrast their functions in vertebrates. We find the loss of zebrafish ns or gnl2 has a major impact on 60S large ribosomal subunit formation and/or function due to cleavage impairments at distinct sites of pre-rRNA transcript. In both cases this leads to a reduction of total protein synthesis. In contrast, gnl3l loss shows relatively minor rRNA processing delays that ultimately have no appreciable effects on ribosome biogenesis or protein synthesis. However, the loss of gnl3l still results in p53 stabilization, apoptosis, and lethality similarly to ns and gnl2 loss. The depletion of p53 in all three of the mutants led to partial rescues of the morphological phenotypes and surprisingly, a rescue of the 60S subunit collapse in the ns mutants. We show that this rescue is due to an unexpected effect of p53 loss that even in wild type embryos results in an increase of 60S subunits. Our study presents an in-depth description of the mechanisms through which ns and gnl2 function in vertebrate ribosome biogenesis and shows that despite the high degree of sequence and domain homology, gnl3l has critical functions in development that are unrelated to the ribosome.

AB - Nucleostemin (NS) is an essential protein for the growth and viability of developmental stem cells. Its functions are multi-faceted, including important roles in ribosome biogenesis and in the p53-induced apoptosis pathway. While NS has been well studied, the functions of its family members GNL2 and GNL3-like (GNL3L) remain relatively obscure despite a high degree of sequence and domain homology. Here, we use zebrafish lines carrying mutations in the ns family to compare and contrast their functions in vertebrates. We find the loss of zebrafish ns or gnl2 has a major impact on 60S large ribosomal subunit formation and/or function due to cleavage impairments at distinct sites of pre-rRNA transcript. In both cases this leads to a reduction of total protein synthesis. In contrast, gnl3l loss shows relatively minor rRNA processing delays that ultimately have no appreciable effects on ribosome biogenesis or protein synthesis. However, the loss of gnl3l still results in p53 stabilization, apoptosis, and lethality similarly to ns and gnl2 loss. The depletion of p53 in all three of the mutants led to partial rescues of the morphological phenotypes and surprisingly, a rescue of the 60S subunit collapse in the ns mutants. We show that this rescue is due to an unexpected effect of p53 loss that even in wild type embryos results in an increase of 60S subunits. Our study presents an in-depth description of the mechanisms through which ns and gnl2 function in vertebrate ribosome biogenesis and shows that despite the high degree of sequence and domain homology, gnl3l has critical functions in development that are unrelated to the ribosome.

KW - Animals

KW - Base Sequence

KW - DNA Primers

KW - Genes, Lethal

KW - Genes, p53

KW - Nuclear Proteins

KW - Polymerase Chain Reaction

KW - RNA, Ribosomal

KW - Ribosomes

KW - Zebrafish

KW - Zebrafish Proteins

KW - Comparative Study

KW - Journal Article

U2 - 10.1016/j.ydbio.2013.10.029

DO - 10.1016/j.ydbio.2013.10.029

M3 - Article

VL - 385

SP - 304

EP - 315

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -

ID: 53430524