Publication

A cellular census of human lungs identifies novel cell states in health and in asthma

Braga, F. A. V., Kar, G., Berg, M., Carpaij, O. A., Polanski, K., Simon, L. M., Brouwer, S., Gomes, T., Hesse, L., Jiang, J., Fasouli, E. S., Efremova, M., Vento-Tormo, R., Talavera-Lopez, C., Jonker, M. R., Affleck, K., Palit, S., Strzelecka, P. M., Firth, H. V., Mahbubani, K. T., Cvejic, A., Meyer, K. B., Saeb-Parsy, K., Luinge, M., Brandsma, C-A., Timens, W., Angelidis, I., Strunz, M., Koppelman, G. H., van Oosterhout, A. J., Schiller, H. B., Theis, F. J., van den Berge, M., Nawijn, M. C. & Teichmann, S. A., Jul-2019, In : Nature Medicine. 25, 7, p. 1153-1163 24 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Braga, F. A. V., Kar, G., Berg, M., Carpaij, O. A., Polanski, K., Simon, L. M., ... Teichmann, S. A. (2019). A cellular census of human lungs identifies novel cell states in health and in asthma. Nature Medicine, 25(7), 1153-1163. https://doi.org/10.1038/s41591-019-0468-5

Author

Braga, Felipe A. Vieira ; Kar, Gozde ; Berg, Marijn ; Carpaij, Orestes A. ; Polanski, Krzysztof ; Simon, Lukas M. ; Brouwer, Sharon ; Gomes, Tomas ; Hesse, Laura ; Jiang, Jian ; Fasouli, Eirini S. ; Efremova, Mirjana ; Vento-Tormo, Roser ; Talavera-Lopez, Carlos ; Jonker, Marnix R. ; Affleck, Karen ; Palit, Subarna ; Strzelecka, Paulina M. ; Firth, Helen V. ; Mahbubani, Krishnaa T. ; Cvejic, Ana ; Meyer, Kerstin B. ; Saeb-Parsy, Kourosh ; Luinge, Marjan ; Brandsma, Corry-Anke ; Timens, Wim ; Angelidis, Ilias ; Strunz, Maximilian ; Koppelman, Gerard H. ; van Oosterhout, Antoon J. ; Schiller, Herbert B. ; Theis, Fabian J. ; van den Berge, Maarten ; Nawijn, Martijn C. ; Teichmann, Sarah A. / A cellular census of human lungs identifies novel cell states in health and in asthma. In: Nature Medicine. 2019 ; Vol. 25, No. 7. pp. 1153-1163.

Harvard

Braga, FAV, Kar, G, Berg, M, Carpaij, OA, Polanski, K, Simon, LM, Brouwer, S, Gomes, T, Hesse, L, Jiang, J, Fasouli, ES, Efremova, M, Vento-Tormo, R, Talavera-Lopez, C, Jonker, MR, Affleck, K, Palit, S, Strzelecka, PM, Firth, HV, Mahbubani, KT, Cvejic, A, Meyer, KB, Saeb-Parsy, K, Luinge, M, Brandsma, C-A, Timens, W, Angelidis, I, Strunz, M, Koppelman, GH, van Oosterhout, AJ, Schiller, HB, Theis, FJ, van den Berge, M, Nawijn, MC & Teichmann, SA 2019, 'A cellular census of human lungs identifies novel cell states in health and in asthma', Nature Medicine, vol. 25, no. 7, pp. 1153-1163. https://doi.org/10.1038/s41591-019-0468-5

Standard

A cellular census of human lungs identifies novel cell states in health and in asthma. / Braga, Felipe A. Vieira; Kar, Gozde; Berg, Marijn; Carpaij, Orestes A.; Polanski, Krzysztof; Simon, Lukas M.; Brouwer, Sharon; Gomes, Tomas; Hesse, Laura; Jiang, Jian; Fasouli, Eirini S.; Efremova, Mirjana; Vento-Tormo, Roser; Talavera-Lopez, Carlos; Jonker, Marnix R.; Affleck, Karen; Palit, Subarna; Strzelecka, Paulina M.; Firth, Helen V.; Mahbubani, Krishnaa T.; Cvejic, Ana; Meyer, Kerstin B.; Saeb-Parsy, Kourosh; Luinge, Marjan; Brandsma, Corry-Anke; Timens, Wim; Angelidis, Ilias; Strunz, Maximilian; Koppelman, Gerard H.; van Oosterhout, Antoon J.; Schiller, Herbert B.; Theis, Fabian J.; van den Berge, Maarten; Nawijn, Martijn C.; Teichmann, Sarah A.

In: Nature Medicine, Vol. 25, No. 7, 07.2019, p. 1153-1163.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Braga FAV, Kar G, Berg M, Carpaij OA, Polanski K, Simon LM et al. A cellular census of human lungs identifies novel cell states in health and in asthma. Nature Medicine. 2019 Jul;25(7):1153-1163. https://doi.org/10.1038/s41591-019-0468-5


BibTeX

@article{4e9b39ddb743409d8cb683594dab9bc6,
title = "A cellular census of human lungs identifies novel cell states in health and in asthma",
abstract = "Human lungs enable efficient gas exchange and form an interface with the environment, which depends on mucosal immunity for protection against infectious agents. Tightly controlled interactions between structural and immune cells are required to maintain lung homeostasis. Here, we use single-cell transcriptomics to chart the cellular landscape of upper and lower airways and lung parenchyma in healthy lungs, and lower airways in asthmatic lungs. We report location-dependent airway epithelial cell states and a novel subset of tissue-resident memory T cells. In the lower airways of patients with asthma, mucous cell hyperplasia is shown to stem from a novel mucous ciliated cell state, as well as goblet cell hyperplasia. We report the presence of pathogenic effector type 2 helper T cells (TH2) in asthmatic lungs and find evidence for type 2 cytokines in maintaining the altered epithelial cell states. Unbiased analysis of cell-cell interactions identifies a shift from airway structural cell communication in healthy lungs to a TH2-dominated interactome in asthmatic lungs.",
keywords = "EPITHELIAL-CELLS, PROSTAGLANDIN D-2, RNA-SEQ, EXPRESSION, INFLAMMATION, PATHOGENESIS, MACROPHAGES, PHENOTYPE, DISEASE, GENE",
author = "Braga, {Felipe A. Vieira} and Gozde Kar and Marijn Berg and Carpaij, {Orestes A.} and Krzysztof Polanski and Simon, {Lukas M.} and Sharon Brouwer and Tomas Gomes and Laura Hesse and Jian Jiang and Fasouli, {Eirini S.} and Mirjana Efremova and Roser Vento-Tormo and Carlos Talavera-Lopez and Jonker, {Marnix R.} and Karen Affleck and Subarna Palit and Strzelecka, {Paulina M.} and Firth, {Helen V.} and Mahbubani, {Krishnaa T.} and Ana Cvejic and Meyer, {Kerstin B.} and Kourosh Saeb-Parsy and Marjan Luinge and Corry-Anke Brandsma and Wim Timens and Ilias Angelidis and Maximilian Strunz and Koppelman, {Gerard H.} and {van Oosterhout}, {Antoon J.} and Schiller, {Herbert B.} and Theis, {Fabian J.} and {van den Berge}, Maarten and Nawijn, {Martijn C.} and Teichmann, {Sarah A.}",
year = "2019",
month = "7",
doi = "10.1038/s41591-019-0468-5",
language = "English",
volume = "25",
pages = "1153--1163",
journal = "Nature Medicine",
issn = "1078-8956",
publisher = "Nature Publishing Group",
number = "7",

}

RIS

TY - JOUR

T1 - A cellular census of human lungs identifies novel cell states in health and in asthma

AU - Braga, Felipe A. Vieira

AU - Kar, Gozde

AU - Berg, Marijn

AU - Carpaij, Orestes A.

AU - Polanski, Krzysztof

AU - Simon, Lukas M.

AU - Brouwer, Sharon

AU - Gomes, Tomas

AU - Hesse, Laura

AU - Jiang, Jian

AU - Fasouli, Eirini S.

AU - Efremova, Mirjana

AU - Vento-Tormo, Roser

AU - Talavera-Lopez, Carlos

AU - Jonker, Marnix R.

AU - Affleck, Karen

AU - Palit, Subarna

AU - Strzelecka, Paulina M.

AU - Firth, Helen V.

AU - Mahbubani, Krishnaa T.

AU - Cvejic, Ana

AU - Meyer, Kerstin B.

AU - Saeb-Parsy, Kourosh

AU - Luinge, Marjan

AU - Brandsma, Corry-Anke

AU - Timens, Wim

AU - Angelidis, Ilias

AU - Strunz, Maximilian

AU - Koppelman, Gerard H.

AU - van Oosterhout, Antoon J.

AU - Schiller, Herbert B.

AU - Theis, Fabian J.

AU - van den Berge, Maarten

AU - Nawijn, Martijn C.

AU - Teichmann, Sarah A.

PY - 2019/7

Y1 - 2019/7

N2 - Human lungs enable efficient gas exchange and form an interface with the environment, which depends on mucosal immunity for protection against infectious agents. Tightly controlled interactions between structural and immune cells are required to maintain lung homeostasis. Here, we use single-cell transcriptomics to chart the cellular landscape of upper and lower airways and lung parenchyma in healthy lungs, and lower airways in asthmatic lungs. We report location-dependent airway epithelial cell states and a novel subset of tissue-resident memory T cells. In the lower airways of patients with asthma, mucous cell hyperplasia is shown to stem from a novel mucous ciliated cell state, as well as goblet cell hyperplasia. We report the presence of pathogenic effector type 2 helper T cells (TH2) in asthmatic lungs and find evidence for type 2 cytokines in maintaining the altered epithelial cell states. Unbiased analysis of cell-cell interactions identifies a shift from airway structural cell communication in healthy lungs to a TH2-dominated interactome in asthmatic lungs.

AB - Human lungs enable efficient gas exchange and form an interface with the environment, which depends on mucosal immunity for protection against infectious agents. Tightly controlled interactions between structural and immune cells are required to maintain lung homeostasis. Here, we use single-cell transcriptomics to chart the cellular landscape of upper and lower airways and lung parenchyma in healthy lungs, and lower airways in asthmatic lungs. We report location-dependent airway epithelial cell states and a novel subset of tissue-resident memory T cells. In the lower airways of patients with asthma, mucous cell hyperplasia is shown to stem from a novel mucous ciliated cell state, as well as goblet cell hyperplasia. We report the presence of pathogenic effector type 2 helper T cells (TH2) in asthmatic lungs and find evidence for type 2 cytokines in maintaining the altered epithelial cell states. Unbiased analysis of cell-cell interactions identifies a shift from airway structural cell communication in healthy lungs to a TH2-dominated interactome in asthmatic lungs.

KW - EPITHELIAL-CELLS

KW - PROSTAGLANDIN D-2

KW - RNA-SEQ

KW - EXPRESSION

KW - INFLAMMATION

KW - PATHOGENESIS

KW - MACROPHAGES

KW - PHENOTYPE

KW - DISEASE

KW - GENE

U2 - 10.1038/s41591-019-0468-5

DO - 10.1038/s41591-019-0468-5

M3 - Article

VL - 25

SP - 1153

EP - 1163

JO - Nature Medicine

JF - Nature Medicine

SN - 1078-8956

IS - 7

ER -

ID: 85876835