Publication

A cellular census of human lungs identifies novel cell states in health and in asthma

Braga, F. A. V., Kar, G., Berg, M., Carpaij, O. A., Polanski, K., Simon, L. M., Brouwer, S., Gomes, T., Hesse, L., Jiang, J., Fasouli, E. S., Efremova, M., Vento-Tormo, R., Talavera-Lopez, C., Jonker, M. R., Affleck, K., Palit, S., Strzelecka, P. M., Firth, H. V., Mahbubani, K. T., Cvejic, A., Meyer, K. B., Saeb-Parsy, K., Luinge, M., Brandsma, C-A., Timens, W., Angelidis, I., Strunz, M., Koppelman, G. H., van Oosterhout, A. J., Schiller, H. B., Theis, F. J., van den Berge, M., Nawijn, M. C. & Teichmann, S. A., Jul-2019, In : Nature Medicine. 25, 7, p. 1153-+ 24 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

  • A cellular census of human lungs identifies novel cell states in health and in asthma

    Final publisher's version, 25 MB, PDF document

    Request copy

DOI

Human lungs enable efficient gas exchange and form an interface with the environment, which depends on mucosal immunity for protection against infectious agents. Tightly controlled interactions between structural and immune cells are required to maintain lung homeostasis. Here, we use single-cell transcriptomics to chart the cellular landscape of upper and lower airways and lung parenchyma in healthy lungs, and lower airways in asthmatic lungs. We report location-dependent airway epithelial cell states and a novel subset of tissue-resident memory T cells. In the lower airways of patients with asthma, mucous cell hyperplasia is shown to stem from a novel mucous ciliated cell state, as well as goblet cell hyperplasia. We report the presence of pathogenic effector type 2 helper T cells (TH2) in asthmatic lungs and find evidence for type 2 cytokines in maintaining the altered epithelial cell states. Unbiased analysis of cell-cell interactions identifies a shift from airway structural cell communication in healthy lungs to a TH2-dominated interactome in asthmatic lungs.

Original languageEnglish
Pages (from-to)1153-+
Number of pages24
JournalNature Medicine
Volume25
Issue number7
Early online date17-Jun-2019
Publication statusPublished - Jul-2019

    Keywords

  • EPITHELIAL-CELLS, PROSTAGLANDIN D-2, RNA-SEQ, EXPRESSION, INFLAMMATION, PATHOGENESIS, MACROPHAGES, PHENOTYPE, DISEASE, GENE
Related Press / Media
  1. UMCG maakt 'plattegrond' van luchtwegen, die kan helpen bij de strijd tegen astma

    Martijn Nawijn

    18/06/2019

    1 item of Media coverage

    Press/Media: ResearchPopular

  2. Atlas van de Longen

    Martijn Nawijn

    18/06/2019

    1 item of Media coverage

    Press/Media: ResearchPopular

  3. Nieuwe celtypen ontdekt in longen

    Martijn Nawijn

    18/06/2019

    1 item of Media coverage

    Press/Media: ResearchPopular

View all (3) »

View graph of relations

ID: 85876835