Publication

A cellular census of healthy lung and asthmatic airway wall identifies novel cell states in health and disease

Braga, F. V., Kar, G., Berg, M., Carpaij, O., polanski, K., Simon, L., Brouwer, S., Gomes, T., Hesse, L., Jiang, J., Fasouli, E. S., Efremova, M., Vento-Tormo, R., Affleck, K., Palit, S., strzelecka, P., Firth, H. V., Mahbubani, K. T. A., Cvejic, A., Meyer, K., Saeb-Parsy, K., Luinge, M., Brandsma, C., Timens, W., Angelidis, I., Strunz, M., Koppelman, G., van Oosterhout, A., Schiller, H. B., Theis, F., van den Berge, M., Nawijn, M. & Teichmann, S. A., 23-Jan-2019, (Submitted) In : bioRxiv. 39 p.

Research output: Contribution to journalArticleAcademic

APA

Braga, F. V., Kar, G., Berg, M., Carpaij, O., polanski, K., Simon, L., Brouwer, S., Gomes, T., Hesse, L., Jiang, J., Fasouli, E. S., Efremova, M., Vento-Tormo, R., Affleck, K., Palit, S., strzelecka, P., Firth, H. V., Mahbubani, K. T. A., Cvejic, A., ... Teichmann, S. A. (2019). A cellular census of healthy lung and asthmatic airway wall identifies novel cell states in health and disease. Manuscript submitted for publication. https://doi.org/10.1101/527408

Author

Braga, Felipe Vieira ; Kar, Gozde ; Berg, Marijn ; Carpaij, Orestes ; polanski, krystof ; Simon, Lucas ; Brouwer, Sharon ; Gomes, Tomas ; Hesse, Laura ; Jiang, J ; Fasouli, E.S. ; Efremova, M ; Vento-Tormo, R ; Affleck, Karen ; Palit, S ; strzelecka, p ; Firth, Helen V. ; Mahbubani, K.T.A. ; Cvejic, Ana ; Meyer, K ; Saeb-Parsy, K ; Luinge, Marjan ; Brandsma, C. ; Timens, Willem ; Angelidis, I ; Strunz, M ; Koppelman, Gerard ; van Oosterhout, Antonius ; Schiller, H. B. ; Theis, F. ; van den Berge, Maarten ; Nawijn, Martijn ; Teichmann, Sarah A. / A cellular census of healthy lung and asthmatic airway wall identifies novel cell states in health and disease. In: bioRxiv. 2019.

Harvard

Braga, FV, Kar, G, Berg, M, Carpaij, O, polanski, K, Simon, L, Brouwer, S, Gomes, T, Hesse, L, Jiang, J, Fasouli, ES, Efremova, M, Vento-Tormo, R, Affleck, K, Palit, S, strzelecka, P, Firth, HV, Mahbubani, KTA, Cvejic, A, Meyer, K, Saeb-Parsy, K, Luinge, M, Brandsma, C, Timens, W, Angelidis, I, Strunz, M, Koppelman, G, van Oosterhout, A, Schiller, HB, Theis, F, van den Berge, M, Nawijn, M & Teichmann, SA 2019, 'A cellular census of healthy lung and asthmatic airway wall identifies novel cell states in health and disease', bioRxiv. https://doi.org/10.1101/527408

Standard

A cellular census of healthy lung and asthmatic airway wall identifies novel cell states in health and disease. / Braga, Felipe Vieira; Kar, Gozde; Berg, Marijn; Carpaij, Orestes; polanski, krystof; Simon, Lucas; Brouwer, Sharon; Gomes, Tomas; Hesse, Laura; Jiang, J; Fasouli, E.S.; Efremova, M; Vento-Tormo, R; Affleck, Karen; Palit, S; strzelecka, p; Firth, Helen V.; Mahbubani, K.T.A.; Cvejic, Ana; Meyer, K; Saeb-Parsy, K; Luinge, Marjan; Brandsma, C.; Timens, Willem; Angelidis, I; Strunz, M; Koppelman, Gerard; van Oosterhout, Antonius; Schiller, H. B.; Theis, F.; van den Berge, Maarten; Nawijn, Martijn; Teichmann, Sarah A.

In: bioRxiv, 23.01.2019.

Research output: Contribution to journalArticleAcademic

Vancouver

Braga FV, Kar G, Berg M, Carpaij O, polanski K, Simon L et al. A cellular census of healthy lung and asthmatic airway wall identifies novel cell states in health and disease. bioRxiv. 2019 Jan 23. https://doi.org/10.1101/527408


BibTeX

@article{7cfc4acfebd743c9baccce237312b56a,
title = "A cellular census of healthy lung and asthmatic airway wall identifies novel cell states in health and disease",
abstract = "Human lungs enable efficient gas exchange, and form an interface with the environment which depends on mucosal immunity for protection against infectious agents. Tightly controlled interactions between structural and immune cells are required to maintain lung homeostasis. Here, we use single cell transcriptomics to chart the cellular landscape of upper and lower airways and lung parenchyma in health. We report location-dependent airway epithelial cell states, and a novel subset of tissue-resident memory T cells. In lower airways of asthma patients, mucous cell hyperplasia is shown to stem from a novel mucous ciliated cell state, as well as goblet cell hyperplasia. We report presence of pathogenic effector Th2 cells in asthma, and find evidence for type-2 cytokines in maintaining the altered epithelial cell states. Unbiased analysis of cell-cell interactions identify a shift from airway structural cell communication in health to a Th2-dominated interactome in asthma.",
author = "Braga, {Felipe Vieira} and Gozde Kar and Marijn Berg and Orestes Carpaij and krystof polanski and Lucas Simon and Sharon Brouwer and Tomas Gomes and Laura Hesse and J Jiang and E.S. Fasouli and M Efremova and R Vento-Tormo and Karen Affleck and S Palit and p strzelecka and Firth, {Helen V.} and K.T.A. Mahbubani and Ana Cvejic and K Meyer and K Saeb-Parsy and Marjan Luinge and C. Brandsma and Willem Timens and I Angelidis and M Strunz and Gerard Koppelman and {van Oosterhout}, Antonius and Schiller, {H. B.} and F. Theis and {van den Berge}, Maarten and Martijn Nawijn and Teichmann, {Sarah A}",
year = "2019",
month = jan,
day = "23",
doi = "10.1101/527408",
language = "English",
journal = "bioRxiv",
publisher = "Cold Spring Harbor Labs Journals",

}

RIS

TY - JOUR

T1 - A cellular census of healthy lung and asthmatic airway wall identifies novel cell states in health and disease

AU - Braga, Felipe Vieira

AU - Kar, Gozde

AU - Berg, Marijn

AU - Carpaij, Orestes

AU - polanski, krystof

AU - Simon, Lucas

AU - Brouwer, Sharon

AU - Gomes, Tomas

AU - Hesse, Laura

AU - Jiang, J

AU - Fasouli, E.S.

AU - Efremova, M

AU - Vento-Tormo, R

AU - Affleck, Karen

AU - Palit, S

AU - strzelecka, p

AU - Firth, Helen V.

AU - Mahbubani, K.T.A.

AU - Cvejic, Ana

AU - Meyer, K

AU - Saeb-Parsy, K

AU - Luinge, Marjan

AU - Brandsma, C.

AU - Timens, Willem

AU - Angelidis, I

AU - Strunz, M

AU - Koppelman, Gerard

AU - van Oosterhout, Antonius

AU - Schiller, H. B.

AU - Theis, F.

AU - van den Berge, Maarten

AU - Nawijn, Martijn

AU - Teichmann, Sarah A

PY - 2019/1/23

Y1 - 2019/1/23

N2 - Human lungs enable efficient gas exchange, and form an interface with the environment which depends on mucosal immunity for protection against infectious agents. Tightly controlled interactions between structural and immune cells are required to maintain lung homeostasis. Here, we use single cell transcriptomics to chart the cellular landscape of upper and lower airways and lung parenchyma in health. We report location-dependent airway epithelial cell states, and a novel subset of tissue-resident memory T cells. In lower airways of asthma patients, mucous cell hyperplasia is shown to stem from a novel mucous ciliated cell state, as well as goblet cell hyperplasia. We report presence of pathogenic effector Th2 cells in asthma, and find evidence for type-2 cytokines in maintaining the altered epithelial cell states. Unbiased analysis of cell-cell interactions identify a shift from airway structural cell communication in health to a Th2-dominated interactome in asthma.

AB - Human lungs enable efficient gas exchange, and form an interface with the environment which depends on mucosal immunity for protection against infectious agents. Tightly controlled interactions between structural and immune cells are required to maintain lung homeostasis. Here, we use single cell transcriptomics to chart the cellular landscape of upper and lower airways and lung parenchyma in health. We report location-dependent airway epithelial cell states, and a novel subset of tissue-resident memory T cells. In lower airways of asthma patients, mucous cell hyperplasia is shown to stem from a novel mucous ciliated cell state, as well as goblet cell hyperplasia. We report presence of pathogenic effector Th2 cells in asthma, and find evidence for type-2 cytokines in maintaining the altered epithelial cell states. Unbiased analysis of cell-cell interactions identify a shift from airway structural cell communication in health to a Th2-dominated interactome in asthma.

U2 - 10.1101/527408

DO - 10.1101/527408

M3 - Article

JO - bioRxiv

JF - bioRxiv

ER -

ID: 77375043