Publication

Topography-mediated myofiber formation and endothelial cell sprouting

Almonacid Suarez, A. M., 2020, [Groningen]: University of Groningen. 152 p.

Research output: ThesisThesis fully internal (DIV)Academic

Copy link to clipboard

Documents

  • Title and contents

    Final publisher's version, 414 KB, PDF document

  • Chapter 1

    Final publisher's version, 1 MB, PDF document

  • Chapter 2

    Final publisher's version, 1 MB, PDF document

  • Chapter 3

    Final publisher's version, 3 MB, PDF document

    Embargo ends: 22/06/2021

    Request copy

  • Chapter 4

    Final publisher's version, 6 MB, PDF document

    Embargo ends: 22/06/2021

    Request copy

  • Chapter 5

    Final publisher's version, 1012 KB, PDF document

  • Summary

    Final publisher's version, 311 KB, PDF document

  • Samenvatting

    Final publisher's version, 312 KB, PDF document

  • Acknowledgements

    Final publisher's version, 317 KB, PDF document

  • Complete thesis

    Final publisher's version, 12 MB, PDF document

    Embargo ends: 22/06/2021

    Request copy

  • Propositions

    Final publisher's version, 116 KB, PDF document

DOI

Directional topography is often used in cell alignment. This thesis explores the influences of directional topography and topography-aligned differentiated myoblasts in combination with endothelial cells in triggering capillary network formation for tissue engineering of skeletal muscle. Directional topography gradients were used to study the optimum alignment of differentiated myoblasts i.e., myotubes. It was shown that human myoblasts aligned and differentiated irrespective of the topography section. Subsequently, endothelial cells (ECs) were added to the same directional topographic systems, showing that ECs formed unstable sprouting networks that aggregated on the smaller topographies and flat parts whereas ECs themselves aligned on the larger topographies. In addition, different coatings were investigated, such as gelatin, fibronectin, and instructive adipose tissue-derived stromal cell (ASC). We identified that the ASC coating stabilized newly the formed sprouting networks. In addition, we tested the hypothesis that “topography-driven aligned human myotubes promote and support vascular network formation as a prelude to in vitro engineered vascularized skeletal muscle”. As a result, we showed that pre-aligned myotubes support early network sprouting of microvascular endothelial cells by providing collagen fibers and laminin but require accessory cells such as pericytes to complete the vascularization process in vitro. In conclusion, our results showed that aligned topography has a tremendous impact on myotube development and endothelial cell guidance. Topographical cues can be stronger than chemical cues and need further study and consideration in tissue engineering of skeletal muscle.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
Supervisors/Advisors
Award date22-Jun-2020
Place of Publication[Groningen]
Publisher
Print ISBNs9789403427478
Electronic ISBNs9789403427485
Publication statusPublished - 2020

Download statistics

No data available

ID: 127414004