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Structural characterization of glucosylated GOS derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes

Pham, H. T., Dijkhuizen, L. & van Leeuwen, S. S. Dec-2018 In : Carbohydrate Research. 470, p. 57-63 7 p.

Research output: Contribution to journalArticle

β-Galacto-oligosaccharides (GOS) are used commercially in infant nutrition, aiming to functionally replace human milk oligosaccharides (hMOS). Glucansucrases Gtf180-ΔN and GtfA-ΔN of Lactobacillus reuteri strains convert sucrose into α-glucans with (α1→6)/(α1→3) and (α1→4)/(α1→6) glucosidic linkages, respectively. Previously we reported that both glucansucrases glucosylate lactose, producing a minimum of 5 compounds (degree of polymerization 3-4) (GL34 mixture) with (α1→2/3/4) linkages. This GL34 mixture exhibited growth stimulatory effects on various probiotic bacteria. Aiming to obtain additional compounds mimicking hMOS in structure and function, we here studied glucosylation of 3 commercially available galactosyl-lactose GOS compounds. Both Gtf180-ΔN and GtfA-ΔN were unable to use 3'-galactosyl-lactose (β3'-GL), but used sucrose to add a single glucose moiety to 4'-galactosyl-lactose (β4'-GL) and 6'-galactosyl-lactose (β6'-GL). β6'-GL was elongated at its reducing glucosyl unit with an (α1→2)-linked moiety and at its non-reducing end with an (α1→4) linked moiety; β4'-GL was only elongated at its reducing end with an (α1→2) linked moiety. Glucansucrases Gtf180-ΔN and GtfA-ΔN thus can be used to produce galactosyl-lactose-derived oligosaccharides containing (α1→2) and (α→4) glucosidic linkages, potentially with valuable bioactive (prebiotic) properties.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalCarbohydrate Research
Volume470
StatePublished - Dec-2018

    Keywords

  • Glucansucrase Gtf180-ΔN, Glucansucrase GtfA-ΔN, Trans-glycosylation, Galactooligosaccharides (GOS), 1H NMR spectroscopy, lactobacillus reuteri 35-5

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