Intraoperative Multispectral Fluorescence Imaging for the Detection of the Sentinel Lymph Node in Cervical Cancer: A Novel Concept

Crane, L. M. A., Themelis, G., Pleijhuis, R. G., Harlaar, N. J., Sarantopoulos, A., Arts, H. J. G., van der Zee, A. G. J., Ntziachristos, V., Vasilis, N. & van Dam, G. M., Oct-2011, In : Molecular Imaging and Biology. 13, 5, p. 1043-1049 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

Real-time intraoperative near-infrared fluorescence (NIRF) imaging is a promising technique for lymphatic mapping and sentinel lymph node (SLN) detection. The purpose of this technical feasibility pilot study was to evaluate the applicability of NIRF imaging with indocyanin green (ICG) for the detection of the SLN in cervical cancer.

In ten patients with early stage cervical cancer, a mixture of patent blue and ICG was injected into the cervix uteri during surgery. Real-time color and fluorescence videos and images were acquired using a custom-made multispectral fluorescence camera system.

Real-time fluorescence lymphatic mapping was observed in vivo in six patients; a total of nine SLNs were detected, of which one (11%) contained metastases. Ex vivo fluorescence imaging revealed the remaining fluorescent signal in 11 of 197 non-sentinel LNs (5%), of which one contained metastatic tumor tissue. None of the non-fluorescent LNs contained metastases.

We conclude that lymphatic mapping and detection of the SLN in cervical cancer using intraoperative NIRF imaging is technically feasible. However, the technique needs to be refined for full applicability in cervical cancer in terms of sensitivity and specificity.

Original languageEnglish
Pages (from-to)1043-1049
Number of pages7
JournalMolecular Imaging and Biology
Issue number5
Publication statusPublished - Oct-2011


  • Cervical cancer, Near-infrared fluorescence, Sentinel lymph node, Multispectral intraoperative imaging, UTERINE CERVIX, TOPOGRAPHIC DISTRIBUTION, RADICAL HYSTERECTOMY, BREAST-CANCER, SQUAMOUS-CELL, CARCINOMA, BIOPSY, LYMPHADENECTOMY, DISSECTION

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