Growth of catalase A and catalase T deficient mutant strains of Saccharomyces cerevisiae on ethanol and oleic acid: Growth profiles and catalase activities in relation to microbody proliferationKlei, I. J. V. D., Rytka, J., Kunau, W. H. & Veenhuis, M., Apr-1990, In : Archives of Microbiology. 153, 5, p. 513-517 5 p.
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The parental strain (A+T+) of Saccharomyces cerevisiae and mutants, deficient in catalase T (A+T-), catalase A (A-T+) or both catalases (A-T-), grew on ethanol and oleic acid with comparable doubling times. Specific activities of catalase were low in glucose- and ethanol-grown cells. In the two oleic acid-grown A+-strains (A+T+ and A+T-) high catalase activities were found; catalase activity invariably remained low in the A-T+ strain and was never detected in the A-T- strain. The levels of β-oxidation enzymes in oleic acid-grown cells of the parental and all mutant strains were not significantly different. However, cytochrome C peroxidase activity had increased 8-fold in oleic acid grown A- strains (A-T+ and A-T-) compared to parental strain cells. The degree of peroxisomal proliferation was comparable among the different strains. Catalase A was shown to be located in peroxisomes. Catalase T is most probably cytosolic in nature and/or present in the periplasmic space.
|Number of pages||5|
|Journal||Archives of Microbiology|
|Publication status||Published - Apr-1990|
- H2O2-Metabolism, Microbodies, β-Oxidation, Catalase T, Catalase A, Saccharomyces cerevisiae
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