Efficient screening methods for glucosyltransferase genes in Lactobacillus strainsKralj, S., van Geel-schutten, GH., van der Maarel, MJEC. & Dijkhuizen, L., Aug-2003, In : Biocatalysis and Biotransformation. 21, 4-5, p. 181-187 7 p.
Research output: Contribution to journal › Article › Academic › peer-review
Limited information is available about homopolysaccharide synthesis in the genus Lactobacillus . Using efficient screening techniques, extracellular glucosyltransferase (GTF) enzyme activity, resulting in alpha-glucan synthesis from sucrose, was detected in various lactobacilli. PCR with degenerate primers based on homologous boxes of known glucosyltransferase (gtf ) genes of lactic acid bacteria strains allowed cloning of fragments of 10 putative gtf genes from eight different glucan producing Lactobacillus strains (five Lactobacillus reuteri strains, one Lactobacillus fermentum strain , one Lactobacillus sake strain and one Lactobacillus parabuchneri strain) . Sequence analysis revealed that these lactobacilli possess a large variation of (putative) gtf genes, similar to what has been observed for Leuconostoc and Streptococcus strains. Homologs of GTFA of Lb. reuteri 121 (synthesizing reuteran, a unique glucan with alpha-(1-->4) and alpha-(1-->6) glycosidic bonds) ( Kralj et al., 2002 ) were found in three of the four other Lb . reuteri strains tested. The other Lactobacillus GTF fragments showed the highest similarity with GTF enzymes of Leuconostoc spp.
|Number of pages||7|
|Journal||Biocatalysis and Biotransformation|
|Publication status||Published - Aug-2003|
|Event||5th Carbohydrate Bioengineering Meeting - , Netherlands|
Duration: 6-Apr-2003 → 9-Apr-2003
5th Carbohydrate Bioengineering Meeting
06/04/2003 → 09/04/2003Netherlands
- glucosyltransferase, Lactobacillus, glucansucrase, sucrose, glucan, polysaccharide, STREPTOCOCCUS-SALIVARIUS ATCC-25975, MESENTEROIDES NRRL B-512F, MOLECULAR CHARACTERIZATION, SEQUENCE-ANALYSIS, NUCLEOTIDE-SEQUENCE, DEXTRANSUCRASE, GLUCAN, EXOPOLYSACCHARIDES, ALPHA(1-6), CLONING