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Efficient screening methods for glucosyltransferase genes in Lactobacillus strains

Kralj, S., van Geel-schutten, GH., van der Maarel, MJEC. & Dijkhuizen, L., Aug-2003, In : Biocatalysis and Biotransformation. 21, 4-5, p. 181-187 7 p.

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Limited information is available about homopolysaccharide synthesis in the genus Lactobacillus . Using efficient screening techniques, extracellular glucosyltransferase (GTF) enzyme activity, resulting in alpha-glucan synthesis from sucrose, was detected in various lactobacilli. PCR with degenerate primers based on homologous boxes of known glucosyltransferase (gtf ) genes of lactic acid bacteria strains allowed cloning of fragments of 10 putative gtf genes from eight different glucan producing Lactobacillus strains (five Lactobacillus reuteri strains, one Lactobacillus fermentum strain , one Lactobacillus sake strain and one Lactobacillus parabuchneri strain) . Sequence analysis revealed that these lactobacilli possess a large variation of (putative) gtf genes, similar to what has been observed for Leuconostoc and Streptococcus strains. Homologs of GTFA of Lb. reuteri 121 (synthesizing reuteran, a unique glucan with alpha-(1-->4) and alpha-(1-->6) glycosidic bonds) ( Kralj et al., 2002 ) were found in three of the four other Lb . reuteri strains tested. The other Lactobacillus GTF fragments showed the highest similarity with GTF enzymes of Leuconostoc spp.

Original languageEnglish
Pages (from-to)181-187
Number of pages7
JournalBiocatalysis and Biotransformation
Volume21
Issue number4-5
Publication statusPublished - Aug-2003
Event5th Carbohydrate Bioengineering Meeting - , Netherlands
Duration: 6-Apr-20039-Apr-2003

Event

5th Carbohydrate Bioengineering Meeting

06/04/200309/04/2003

Netherlands

Event: Other

    Keywords

  • glucosyltransferase, Lactobacillus, glucansucrase, sucrose, glucan, polysaccharide, STREPTOCOCCUS-SALIVARIUS ATCC-25975, MESENTEROIDES NRRL B-512F, MOLECULAR CHARACTERIZATION, SEQUENCE-ANALYSIS, NUCLEOTIDE-SEQUENCE, DEXTRANSUCRASE, GLUCAN, EXOPOLYSACCHARIDES, ALPHA(1-6), CLONING

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