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BRCA2 deficiency instigates cGAS-mediated inflammatory signaling and confers sensitivity to tumor necrosis factor-alpha-mediated cytotoxicity

Heijink, A. M., Talens, F., Jae, L. T., van Gijn, S. E., Fehrmann, R. S. N., Brummelkamp, T. R. & van Vugt, M. A. T. M., 9-Jan-2019, In : Nature Communications. 10, 1, 14 p., 100.

Research output: Contribution to journalArticleAcademicpeer-review

Loss of BRCA2 affects genome stability and is deleterious for cellular survival. Using a genome-wide genetic screen in near-haploid KBM-7 cells, we show that tumor necrosis factor-alpha (TNF alpha) signaling is a determinant of cell survival upon BRCA2 inactivation. Specifically, inactivation of the TNF receptor (TNFR1) or its downstream effector SAM68 rescues cell death induced by BRCA2 inactivation. BRCA2 inactivation leads to proinflammatory cytokine production, including TNF alpha, and increases sensitivity to TNF alpha. Enhanced TNF alpha sensitivity is not restricted to BRCA2 inactivation, as BRCA1 or FANCD2 inactivation, or hydroxyurea treatment also sensitizes cells to TNF alpha. Mechanistically, BRCA2 inactivation leads to cGAS-positive micronuclei and results in a cell-intrinsic interferon response, as assessed by quantitative mass-spectrometry and gene expression profiling, and requires ASK1 and JNK signaling. Combined, our data reveals that micronuclei induced by loss of BRCA2 instigate a cGAS/STING-mediated interferon response, which encompasses rewired TNF alpha signaling and enhances TNF alpha sensitivity.

Original languageEnglish
Article number100
Number of pages14
JournalNature Communications
Volume10
Issue number1
Publication statusPublished - 9-Jan-2019

    Keywords

  • NF-KAPPA-B, CANCER SUSCEPTIBILITY GENE, EMBRYONIC CELLULAR PROLIFERATION, SUSTAINED JNK ACTIVATION, DNA-DAMAGE RESPONSE, STRAND BREAK REPAIR, HOMOLOGOUS RECOMBINATION, CHROMOSOMAL INSTABILITY, MICE LACKING, HUMAN-CELLS

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