A Systematic Protein Refolding Screen Method using the DGR Approach Reveals that Time and Secondary TSA are Essential VariablesWang, Y., van Oosterwijk, N., Ali, A. M., Adawy, A., Anindya, A. L., Dömling, A. S. S. & Groves, M. R. 24-Aug-2017 In : Scientific Reports. 7, 1, 10 p., 9355
Research output: Scientific - peer-review › Article
Refolding of proteins derived from inclusion bodies is very promising as it can provide a reliable source of target proteins of high purity. However, inclusion body-based protein production is often limited by the lack of techniques for the detection of correctly refolded protein. Thus, the selection of the refolding conditions is mostly achieved using trial and error approaches and is thus a time-consuming process. In this study, we use the latest developments in the differential scanning fluorimetry guided refolding approach as an analytical method to detect correctly refolded protein. We describe a systematic buffer screen that contains a 96-well primary pH-refolding screen in conjunction with a secondary additive screen. Our research demonstrates that this approach could be applied for determining refolding conditions for several proteins. In addition, it revealed which "helper" molecules, such as arginine and additives are essential. Four different proteins: HA-RBD, MDM2, IL-17A and PD-L1 were used to validate our refolding approach. Our systematic protocol evaluates the impact of the "helper" molecules, the pH, buffer system and time on the protein refolding process in a high-throughput fashion. Finally, we demonstrate that refolding time and a secondary thermal shift assay buffer screen are critical factors for improving refolding efficiency.
|Number of pages||10|
|State||Published - 24-Aug-2017|
- Journal Article