Publication

A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing

Fajrial, A. K., He, Q. Q., Wirusanti, N., Slansky, J. E. & Ding, X., 2020, In : Theranostics. 10, 12, p. 5532-5549 18 p.

Research output: Contribution to journalReview articleAcademicpeer-review

  • Apresio K. Fajrial
  • Qing Qing He
  • Nurul Wirusanti
  • Jill E. Slansky
  • Xiaoyun Ding

Gene editing is a versatile technique in biomedicine that promotes fundamental research as well as clinical therapy. The development of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) as a genome editing machinery has accelerated the application of gene editing. However, the delivery of CRISPR components often suffers when using conventional transfection methods, such as viral transduction and chemical vectors, due to limited packaging size and inefficiency toward certain cell types. In this review, we discuss physical transfection methods for CRISPR gene editing which can overcome these limitations. We outline different types of physical transfection methods, highlight novel techniques to deliver CRISPR components, and emphasize the role of micro and nanotechnology to improve transfection performance. We present our perspectives on the limitations of current technology and provide insights on the future developments of physical transfection methods.

Original languageEnglish
Pages (from-to)5532-5549
Number of pages18
JournalTheranostics
Volume10
Issue number12
Publication statusPublished - 2020

    Keywords

  • physical transfection, CRISPR delivery, intracellular delivery, gene editing, transfection methods, micro/nanotechnology, EFFICIENT INTRACELLULAR DELIVERY, LIPID-MEDIATED DNA, SINGLE-CELL LEVEL, HIGHLY EFFICIENT, POLYETHYLENIMINE PEI, DIRECT VISUALIZATION, IN-VITRO, CRISPR, ELECTROPORATION, RNA

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