Dataset: Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice
Amssoms, K. (Creator), Born, P. (Creator), Beugeling, M. (Creator), De Clerck, B. (Creator), Van Gulck, E. (Creator), Hinrichs, W. (Creator), Frijlink, E. (Creator), Grasmeijer, N. (Creator), Kraus, G. (Creator), Sutmuller, R. (Creator), Simmen, K. (Creator), Baert, L. (Creator), University of Groningen, 17-Aug-2018
In this study we investigated whether a core-shell based implant, containing ovalbumin as core material and poly(DL-lactic-co-glycolic acid) of various monomer ratios as shell material can be used to obtain such a booster release. An in vitro release study showed that the lag time after which the ovalbumin was released from the core-shell implant increased with increasing lactic to glycolic acid ratio of the polymer and ranged from 3–6 weeks. Fluorescence spectroscopy showed minimal differences between native ovalbumin and ovalbumin from core-shell implants that were incubated until just before the observed in vitro release. In addition, mice immunized with a subcutaneous inserted core-shell implant containing ovalbumin showed an ovalbumin-specific IgG1 antibody response after a lag time of 4 or 6–8 weeks.
|Date made available||17-Aug-2018|
|Publisher||University of Groningen|
|Access to the dataset||Open|
- biogradable polymer, biphasic pulsatile release, implant, poly(lactic-co-glycolic acid), prime-boost, single-injection, antigen, vaccine
Keywords on Datasets
Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in miceAmssoms, K., Born, P. A., Beugeling, M., De Clerck, B., Van Gulck, E., Hinrichs, W. L. J., Frijlink, H. W., Grasmeijer, N., Kraus, G., Sutmuller, R., Simmen, K. & Baert, L., 30-Aug-2018, In : PLoS ONE. 13, 8, p. e0202961 14 p., 0202961.
Research output: Contribution to journal › Article › Academic › peer-review