Dataset

Data from: Ultra-long telomeres shorten with age in nestling great tits but are static in adults and mask attrition of short telomeres

Atema, E. (Creator), Mulder, E. (Creator), van Noordwijk, A. J. (Creator), Verhulst, S. (Creator), University of Groningen, 14-Jan-2019

Dataset

  • Elsje Atema Department of Animal Ecology, Netherlands Institute of Ecology (NIOO-KNAW), P.O. Box 50, 6700 AB Wageningen, The Netherlands (Creator)
  • Ellis Mulder (Creator)
  • Arie J. van Noordwijk (Creator)
  • Simon Verhulst (Creator)
  • Department of Animal Ecology, Netherlands Institute of Ecology (NIOO-KNAW), P.O. Box 50, 6700 AB Wageningen, The Netherlands

Description

Telomere length (TL) is increasingly used as a biomarker of senescence, but measuring telomeres remains a challenge. Within tissue samples, TL varies between cells and chromosomes. Class I telomeres are (presumably static) interstitial telomeric sequences, and terminal telomeres have been divided in shorter (Class II) telomeres and ultra-long (Class III) telomeres, and the presence of the latter varies strongly between species. Class II telomeres typically shorten with age, but little is known of Class III telomere dynamics. Using multiple experimental approaches, we show great tits to have ultra-long telomeres, and we investigated age effects on Class II and III telomeres using a longitudinal approach (our method excludes Class I telomeres). In adults, TL averaged over the whole distribution did not significantly change with age.

The data package contains three datasets:
- The file TLadults.xlsx contains the information on the telomere measurements (TRF in bp) in adults with age in years. The file contains three sheets: (1) measurements of the complete telomere range, (2) measurements of the ‘smear’ at the lower end of the distribution only, (3) measurements of the telomere range above the ‘smear’.
- The file “qPCRdata.xlsx” contains telomere length data measured using two different techniques, TRF (in bp) and qPCR, for great tits and blue tits (i.e. the data used for figure 4 and associated analyses).
- The file TLchicks contains the information on the telomere measurements (TRF in bp) in nestlings with age in days. The file contains three sheets: (1) measurements of the complete telomere range, (2) measurements of the ‘smear’ at the lower end of the distribution only, (3) measurements of the telomere range above the ‘smear’.
Date made available14-Jan-2019
PublisherUniversity of Groningen
Geographical coverageVlieland, Netherlands
Access to the dataset Open
Contact researchdata@rug.nl

    Keywords on Datasets

  • TRF, telomere distribution, micro-chromosomes, Birds, qPCR, Parus major, Cyanistes caeruleus
Related Publications
  1. Ultralong telomeres shorten with age in nestling great tits but are static in adults and mask attrition of short telomeres

    Atema, E., Mulder, E., van Noordwijk, A. J. & Verhulst, S., May-2019, In : Molecular Ecology Resources. 19, 3, p. 648-658 11 p.

    Research output: Contribution to journalArticleAcademicpeer-review

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