Data from: Development of a Nasonia vitripennis outbred laboratory population for genetic analysis

Jacobus Mgn Van De Zande, L. (Creator), Ferber, S. (Creator), de Haan, A. (Creator), Beukeboom, L. (Creator), van Heerwaarden, J. (Creator), Pannebakker, B. (Creator), University of Groningen, 12-Nov-2013


  • Louis Jacobus Mgn Van De Zande (Creator)
  • Steven Ferber (Creator)
  • Ammerins de Haan (Creator)
  • Leo Beukeboom (Creator)
  • Joost van Heerwaarden (Creator)
  • Bartholomeus Pannebakker Wageningen Univ, Wageningen University & Research Center, Genet Lab (Creator)
  • Wageningen Univ, Wageningen University & Research Center, Genet Lab


The parasitoid wasp genus Nasonia has rapidly become a genetic model system for developmental and evolutionary biology. The release of its genome sequence led to the development of high-resolution genomic tools, for both interspecific and intraspecific research, which has resulted in great advances in understanding Nasonia biology. To further advance the utility of Nasonia vitripennis as a genetic model system and to be able to fully exploit the advantages of its fully sequenced and annotated genome, we developed a genetically variable and well-characterized experimental population. In this study, we describe the establishment of the genetically diverse HVRx laboratory population from strains collected from the field in the Netherlands. We established a maintenance method that retains genetic variation over generations of culturing in the laboratory. As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (Ne) by microsatellite analysis. A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417 331 high-quality SNPs spanning all five Nasonia chromosomes. The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system.

The data package contains five sets:
- Nasonia vitripennis HVRx outbred laboratory population SNP data - Chromosome 1. VarScan.v2.2.8 output according to the following rules: minimum consensus quality: 20; minimum count of minor allele: 2, minimum coverage: 8. Reads with mapping qualities less than 20 were discarded.
Date made available12-Nov-2013
PublisherUniversity of Groningen
Geographical coverageHoge Veluwe, The Netherlands
Access to the dataset Open

    Keywords on Datasets

  • parasitoid wasp, single nucleotide polymorphism (SNP), pooled resequencing, genetic variation, effective population size, laboratory strain, Nasonia vitripennis
Related Publications
  1. Development of a Nasonia vitripennis outbred laboratory population for genetic analysis

    van de Zande, L., Ferber, S., de Haan, A., Beukeboom, L. W., van Heerwaarden, J. & Pannebakker, B. A., May-2014, In : Molecular Ecology Resources. 14, 3, p. 578-587 10 p.

    Research output: Contribution to journalArticleAcademicpeer-review

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