Data from: Development of a Nasonia vitripennis outbred laboratory population for genetic analysis

Jacobus Mgn Van De Zande, L. (Creator), Ferber, S. (Creator), de Haan, A. (Creator), Beukeboom, L. (Creator), van Heerwaarden, J. (Creator) & Pannebakker, B. (Creator), University of Groningen, 12-Nov-2013


  • Louis Jacobus Mgn Van De Zande (Creator)
  • Steven Ferber (Creator)
  • Ammerins de Haan (Creator)
  • Leo Beukeboom (Creator)
  • Joost van Heerwaarden (Creator)
  • Bartholomeus Pannebakker Wageningen Univ, Wageningen University & Research Center, Genet Lab (Creator)
  • Wageningen Univ, Wageningen University & Research Center, Genet Lab


The parasitoid wasp genus Nasonia has rapidly become a genetic model system for developmental and evolutionary biology. The release of its genome sequence led to the development of high-resolution genomic tools, for both interspecific and intraspecific research, which has resulted in great advances in understanding Nasonia biology. To further advance the utility of Nasonia vitripennis as a genetic model system and to be able to fully exploit the advantages of its fully sequenced and annotated genome, we developed a genetically variable and well-characterized experimental population. In this study, we describe the establishment of the genetically diverse HVRx laboratory population from strains collected from the field in the Netherlands. We established a maintenance method that retains genetic variation over generations of culturing in the laboratory. As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (Ne) by microsatellite analysis. A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417 331 high-quality SNPs spanning all five Nasonia chromosomes. The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system.

The data package contains five sets:
- Nasonia vitripennis HVRx outbred laboratory population SNP data - Chromosome 1. VarScan.v2.2.8 output according to the following rules: minimum consensus quality: 20; minimum count of minor allele: 2, minimum coverage: 8. Reads with mapping qualities less than 20 were discarded.
Date made available12-Nov-2013
PublisherUniversity of Groningen
Geographical coverageHoge Veluwe, The Netherlands
Access to the dataset Open

    Keywords on Datasets

  • parasitoid wasp, single nucleotide polymorphism (SNP), pooled resequencing, genetic variation, effective population size, laboratory strain, Nasonia vitripennis
Related Publications
  1. Development of a Nasonia vitripennis outbred laboratory population for genetic analysis

    van de Zande, L., Ferber, S., de Haan, A., Beukeboom, L. W., van Heerwaarden, J. & Pannebakker, B. A., May-2014, In : Molecular Ecology Resources. 14, 3, p. 578-587 10 p.

    Research output: Contribution to journalArticleAcademicpeer-review

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