Dataset
Data from: Development of a Nasonia vitripennis outbred laboratory population for genetic analysis
Jacobus Mgn Van De Zande, L. (Creator), Ferber, S. (Creator), de Haan, A. (Creator), Beukeboom, L. (Creator), van Heerwaarden, J. (Creator) & Pannebakker, B. (Creator), University of Groningen, 12-Nov-2013
DOI: https://doi.org/10.5061/dryad.f2330, http://hymenopteragenome.org/Nasonia/
Dataset
Links
http://hymenopteragenome.org/Nasonia/
Sequence data in the NasoniaBase
- Louis Jacobus Mgn Van De Zande (Creator)
- Steven Ferber (Creator)
- Ammerins de Haan (Creator)
- Leo Beukeboom (Creator)
- Joost van Heerwaarden (Creator)
- Bartholomeus Pannebakker Wageningen Univ, Wageningen University & Research Center, Genet Lab (Creator)
- Wageningen Univ, Wageningen University & Research Center, Genet Lab
Description
The parasitoid wasp genus Nasonia has rapidly become a genetic model system for developmental and evolutionary biology. The release of its genome sequence led to the development of high-resolution genomic tools, for both interspecific and intraspecific research, which has resulted in great advances in understanding Nasonia biology. To further advance the utility of Nasonia vitripennis as a genetic model system and to be able to fully exploit the advantages of its fully sequenced and annotated genome, we developed a genetically variable and well-characterized experimental population. In this study, we describe the establishment of the genetically diverse HVRx laboratory population from strains collected from the field in the Netherlands. We established a maintenance method that retains genetic variation over generations of culturing in the laboratory. As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (Ne) by microsatellite analysis. A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417 331 high-quality SNPs spanning all five Nasonia chromosomes. The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system.
The data package contains five sets:
- Nasonia vitripennis HVRx outbred laboratory population SNP data - Chromosome 1. VarScan.v2.2.8 output according to the following rules: minimum consensus quality: 20; minimum count of minor allele: 2, minimum coverage: 8. Reads with mapping qualities less than 20 were discarded.
The data package contains five sets:
- Nasonia vitripennis HVRx outbred laboratory population SNP data - Chromosome 1. VarScan.v2.2.8 output according to the following rules: minimum consensus quality: 20; minimum count of minor allele: 2, minimum coverage: 8. Reads with mapping qualities less than 20 were discarded.
Date made available | 12-Nov-2013 |
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Publisher | University of Groningen |
Geographical coverage | Hoge Veluwe, The Netherlands |
Access to the dataset | Open |
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Contact | researchdata@rug.nl |
- parasitoid wasp, single nucleotide polymorphism (SNP), pooled resequencing, genetic variation, effective population size, laboratory strain, Nasonia vitripennis
Keywords on Datasets
Related Publications
Development of a Nasonia vitripennis outbred laboratory population for genetic analysis
van de Zande, L., Ferber, S., de Haan, A., Beukeboom, L. W., van Heerwaarden, J. & Pannebakker, B. A., May-2014, In : Molecular Ecology Resources. 14, 3, p. 578-587 10 p.Research output: Contribution to journal › Article › Academic › peer-review
ID: 66301595