Dataset

Biomedical datasets to: Functional proteomics identifies acinus L as a direct insulin- and amino acid-dependent mTORC1 substrate

Schwarz, J. J. (Creator), Wiese, H. (Creator), Tölle, R. C. (Creator), Zarei, M. (Creator), Dengjel, J. (Creator), Warscheid, B. (Creator), Thedieck, K. (Creator), European Bioinformatics Institute (EMBL-EBI), 28-Apr-2015

Dataset

  • Friedel Drepper (Data Manager)
  • Jennifer Jasmin Schwarz (Creator)
  • Heike Wiese (Creator)
  • Regine Charlotte Tölle (Creator)
  • Mostafa Zarei (Creator)
  • Jörn Dengjel (Creator)
  • Bettina Warscheid (Creator)
  • Kathrin Thedieck (Creator)
  • Albert Ludwigs Univ Freiburg, University of Freiburg, Dept Bioinformat & Mol Genet, Fac Biol, Inst Biol 3

Description

The serine/threonine kinase mammalian target of rapamycin (mTOR) governs growth, metabolism, and aging in response to insulin and amino acids (aa), and is often activated in metabolic disorders and cancer. Much is known about the regulatory signaling network around mTOR, but surprisingly few direct mTOR substrates have been established to date. To tackle this gap in our knowledge, we took advantage of a combined quantitative phosphoproteomic and interactomic strategy. We analyzed the insulin- and aa-responsive phosphoproteome upon inhibition of the mTOR complex 1 (mTORC1) component raptor, and analyzed in parallel the interactome of endogenous mTOR. By overlaying these two datasets, we identified acinus L as a potential novel mTORC1 target. We confirmed acinus L as a direct mTORC1 substrate by co-immunoprecipitation and MS-enhanced kinase assays. Our study delineates a triple proteomics strategy of combined phosphoproteomics, interactomics, and MS-enhanced kinase assays for the de novo-identification of mTOR network components, and provides a rich source of potential novel mTOR interactors and targets for future investigation.
Date made available28-Apr-2015
PublisherEuropean Bioinformatics Institute (EMBL-EBI)
Access to the dataset Open
Contact researchdata@rug.nl

    Keywords on Datasets

  • mass spectrometry proteomics data, Human, Epithelial cell, LTQ FT, phosphorylated residue, monohydroxylated reseidue, SILAC, Shotgun proteomics, LTQ Orbitrap, iodoacetamide derivatized residue, Affinity purification
Related Publications
  1. Functional Proteomics Identifies Acinus L as a Direct Insulin- and Amino Acid-Dependent Mammalian Target of Rapamycin Complex 1 (mTORC1) Substrate

    Schwarz, J. J., Wiese, H., Toelle, R. C., Zarei, M., Dengjel, J., Warscheid, B. & Thedieck, K., Aug-2015, In : Molecular & Cellular Proteomics. 14, 8, p. 2042-2055 14 p.

    Research output: Contribution to journalArticleAcademicpeer-review

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ID: 67045606