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ResearchChemical BiologyProf.Dr. Maglia group

Chemical Biology - Prof.Dr. Maglia group


The main focus of our group is on the chemistry and biophysics of nanopores. We are engineering, modifying and redesigning these protein systems for applications in NanoBioTechnology and Single-Molecule Biophysics.

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Publication from RUG (since end 2014)  


Zhao, S., Restrepo-Pérez, L., Soskine, M., Maglia, G., Joo, C., Dekker, C., & Aksimentiev, A. (2019). Electro-Mechanical Conductance Modulation of a Nanopore Using a Removable Gate. Acs Nano, 13(2), 2398-2409. [acsnano.8b09266].
Huang, G., Voet, A., & Maglia, G. (2019). FraC nanopores with adjustable diameter identify the mass of opposite-charge peptides with 44 dalton resolution. Nature Communications, 10, [835].


Mutter, N. L., Soskine, M., Huang, G., Albuquerque, I. S., Bernardes, G. J. L., & Maglia, G. (2018). Modular pore-forming immunotoxins with caged cytotoxicity tailored by directed evolution. ACS chemical biology, 13(11), 3153–3160. [8b00720].
Galenkamp, N. S., Soskine, M., Hermans, J., Wloka, C., & Maglia, G. (2018). Direct electrical quantification of glucose and asparagine from bodily fluids using nanopores. Nature Communications, 9(1), [4085].
Nomidis, S. K., Hooyberghs, J., Maglia, G., & Carlon, E. (2018). DNA capture into the ClyA nanopore: Diffusion-limited versus reaction-limited processes. Journal of Physics-Condensed Matter, 30(30), [304001].
Maglia, G., Wloka, C., Mutter, N. L., Soskine, M., & Huang, G. (2018). Biological nanopores for biopolymer sensing and sequencing based on frac actinoporin. (Patent No. WO2018012963).
Wilmaerts, D., Bayoumi, M., Dewachter, L., Knapen, W., Mika, J. T., Hofkens, J., ... Michiels, J. (2018). The Persistence-Inducing Toxin HokB Forms Dynamic Pores That Cause ATP Leakage. Mbio, 9(4), [ARTN e00744-18].


Van Meervelt, V., Soskine, M., Singh, S., Schuurman-Wolters, G., Wijma, H. J., Poolman, B., & Maglia, G. (2017). Real-time conformational changes and controlled orientation of native proteins inside a protein nanoreactor. Journal of the American Chemical Society, 139(51), 18640-18646. [jacs.7b10106].
Huang, G., Willems, K., Soskine, M., Wloka, C., & Maglia, G. (2017). Electro-osmotic capture and ionic discrimination of peptide and protein biomarkers with FraC nanopores. Nature Communications, 8, 1-11. [935].
Aminipour, Z., Khorshid, M., Bayoumi, M., Losada-Perez, P., Thoelen, R., Bonakdar, S., ... Van der Bruggen, B. (2017). Formation and electrical characterization of black lipid membranes in porous filter materials. Physica Status Solidi A-Applications and materials science, 214(9), [1700104].
Willems, K., Van Meervelt, V., Wloka, C., & Maglia, G. (2017). Single-molecule nanopore enzymology. Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 372(1726), [20160230].
Bayoumi, M., Bayley, H., Maglia, G., & Sapra, K. T. (2017). Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells. Scientific Reports, 7, 1-11. [45167].
Wloka, C., Van Meervelt, V., van Gelder, D., Danda, N., Jager, N., Williams, C. P., & Maglia, G. (2017). Label-Free and Real-Time Detection of Protein Ubiquitination with a Biological Nanopore. Acs Nano, 11(5), 4387-4394. [acsnano.6b07760].
Loveridge, E. J., Hroch, L., Hughes, R. L., Williams, T., Davies, R. L., Angelastro, A., ... Allemann, R. K. (2017). Reduction of Folate by Dihydrofolate Reductase from Thermotoga maritima. Biochemistry, 56(13), 1879-1886. [acs.biochem.6b01268].


Maglia, G., Soskine, M., Biesemans, A., Meervelt, V., & Poolman, B. (2016). Nanopores with internal protein adaptors. (Patent No. WO2016166232).
Wloka, C., Mutter, N. L., Soskine, M., & Maglia, G. (2016). Alpha-Helical Fragaceatoxin C Nanopore Engineered for Double-Stranded and Single-Stranded Nucleic Acid Analysis. Angewandte Chemie - International Edition, 55(40), 12494-12498.
Serra-Batiste, M., Ninot-Pedrosa, M., Bayoumi, M., Gairí, M., Maglia, G., & Carulla, N. (2016). Aβ42 assembles into specific β-barrel pore-forming oligomers in membrane-mimicking environments. Proceedings of the National Academy of Sciences of the United States of America, 113(39), 10866-10871.
Franceschini, L., Brouns, T., Willems, K., Carlon, E., & Maglia, G. (2016). DNA Translocation through Nanopores at Physiological Ionic Strengths Requires Precise Nanoscale Engineering. Acs Nano, 10(9), 8394-8402.
Biesemans, A., Soskine, M., & Maglia, G. (2016). Controlling the Nanoscopic Confinement of Enzymes Inside ClyA Nanopores for Single-Protein Studies. Biophysical Journal, 110(3, Supplement 1), 518A-518A.
Maglia, G. (2016). Design and Engineering of Nanopores with Emergent Functions. Biophysical Journal, 110(3), 536A-536A.
Vega, M., Perez, M. S., Granell, P., Golmar, F., Wloka, C., Maglia, G., ... Lerner, B. (2016). Effect of butanol and salt concentration on solid-state nanopores resistance. Cogent chemistry, 2, [1225345].


McGinn, S., Bauer, D., Brefort, T., Dong, L., El-Sagheer, A., Elsharawy, A., ... Gut, I. G. (2016). New Technologies for DNA analysis-A review of the READNA Project. New Biotechnology, 33(3), 311-330.
Ho, C-W., Meervelt, V., Tsai, K-C., De Temmerman, P-J., Mast, J., & Maglia, G. (2015). Engineering a nanopore with co-chaperonin function. Science Advances, 1(11), [e1500905].
Biesemans, A., Soskine, M., & Maglia, G. (2015). A Protein Rotaxane Controls the Translocation of Proteins Across a ClyA Nanopore. Nano Letters.
Soskine, M., Biesemans, A., & Maglia, G. (2015). Single-Molecule Analyte Recognition with ClyA Nanopores Equipped with Internal Protein Adaptors. Journal of the American Chemical Society, 137(17), 5793-7.
Stoddart, D., Franceschini, L., Heron, A., Bayley, H., & Maglia, G. (2015). DNA stretching and optimization of nucleobase recognition in enzymatic nanopore sequencing. Nanotechnology, 26(8), [084002].


Van Meervelt, V., Soskine, M., & Maglia, G. (2014). Detection of Two Isomeric Binding Configurations in a Protein-Aptamer Complex with a Biological Nanopore. Acs Nano, 8(12), 12826-12835.
Maglia, G., & Soskine, M. (2014). Nanopore biosensors for detection of proteins and nucleic acids. (Patent No. WO2014153625).
Stoddart, D., Ayub, M., Hoefler, L., Raychaudhuri, P., Klingelhoefer, J. W., Maglia, G., ... Bayley, H. (2014). Functional truncated membrane pores. Proceedings of the National Academy of Science of the United States of America, 111(7), 2425-2430.


Soskine, M., Biesemans, A., De Maeyer, M., & Maglia, G. (2013). Tuning the size and properties of ClyA nanopores assisted by directed evolution. Journal of the American Chemical Society, 135(36), 13456-13463.
Franceschini, L., Soskine, M., Biesemans, A., & Maglia, G. (2013). A nanopore machine promotes the vectorial transport of DNA across membranes. Nature Communications, 4, 1-8. [2415].


Soskine, M., Biesemans, A., Moeyaert, B., Cheley, S., Bayley, H., & Maglia, G. (2012). An engineered ClyA nanopore detects folded target proteins by selective external association and pore entry. Nano Letters, 12(9), 4895-4900.
Fransceschini, L., Mikhailova, E., Bayley, H., & Maglia, G. (2012). Nucleobase recognition at alkaline pH and apparent pK(a) of single DNA bases immobilised within a biological nanopore. Chemical Communications, 48(10), 1520-1522.


Rincon-Restrepo, M., Milthallova, E., Bayley, H., & Maglia, G. (2011). Controlled Translocation of Individual DNA Molecules through Protein Nanopores with Engineered Molecular Brakes. Nano Letters, 11(2), 746-750.


Stoddart, D., Heron, A., Klingelhoefer, J., Mikhailova, E., Maglia, G., & Bayley, H. (2010). Nucleobase Recognition in ssDNA at the Central Constriction of the alpha-Hemolysin Pore. Nano Letters, 10(9), 3633-3637.
Japrung, D., Henricus, M., Li, QH., Maglia, G., & Bayley, H. (2010). Urea Facilitates the Translocation of Single-Stranded DNA and RNA Through the alpha-Hemolysin Nanopore. Biophysical Journal, 98(9), 1856-1863.
Maglia, G., Heron, AJ., Stoddart, D., Japrung, D., & Bayley, H. (2010). ANALYSIS OF SINGLE NUCLEIC ACID MOLECULES WITH PROTEIN NANOPORES. Methods in Enzymology, 475, 591-623.
Wallace, E. VB., Stoddart, D., Heron, A., Mikhailova, E., Maglia, G., Donohoe, T. J., & Bayley, H. (2010). Identification of epigenetic DNA modifications with a protein nanopore. Chemical Communications, 46(43), 8195-8197.
Stoddart, D., Maglia, G., Mikhailova, E., Heron, A., & Bayley, H. (2010). Multiple Base-Recognition Sites in a Biological Nanopore: Two Heads are Better than One. Angewandte Chemie - International Edition, 49(3), 556-559.


Loveridge, EJ., Maglia, G., & Allemann, RK. (2009). The Role of Arginine 28 in Catalysis by Dihydrofolate Reductase from the Hyperthermophile Thermotoga maritima. ChemBioChem, 10(16), 2624-2627.
Maglia, G., Henricus, M., Wyss, R., Li, QH., Cheley, S., & Bayley, H. (2009). DNA Strands from Denatured Duplexes are Translocated through Engineered Protein Nanopores at Alkaline pH. Nano Letters, 9(11), 3831-3836.
Maglia, G., Heron, AJ., Hwang, WL., Holden, MA., Mikhailova, E., Li, QH., ... Bayley, H. (2009). Droplet networks with incorporated protein diodes show collective properties. Nature Nanotechnology, 4(7), 437-440.
Stoddart, D., Heron, AJ., Mikhailova, E., Maglia, G., & Bayley, H. (2009). Single-nucleotide discrimination in immobilized DNA oligonucleotides with a biological nanopore. Proceedings of the National Academy of Science of the United States of America, 106(19), 7702-7707.


Maglia, G., Restrepo, MR., Mikhailova, E., & Bayley, H. (2008). Enhanced translocation of single DNA molecules through alpha-hemolysin nanopores by manipulation of internal charge. Proceedings of the National Academy of Science of the United States of America, 105(50), 19720-19725.
Maglia, G., Jonkheer, A., De Maeyer, M., Frere, JM., & Engelborghs, Y. (2008). An unusual red-edge excitation and time-dependent Stokes shift in the single tryptophan mutant protein DD-carboxypeptidase from Streptomyces: The role of dynamics and tryptophan rotamers. Protein Science, 17(2), 352-361.


WU, HC., Astier, Y., Maglia, G., Mikhailova, E., & Bayley, H. (2007). Protein nanopores with covalently attached molecular adapters. Journal of the American Chemical Society, 129(51), 16142-16148.


Allemann, RK., Evans, RM., Tey, LH., Maglia, G., Pang, JY., Rodriguez, R., ... Swanwick, RS. (2006). Protein motions during catalysis by dihydrofolate reductases. Philosophical Transactions of the Royal Society B: Biological Sciences, 361(1472), 1317-1321.
Swanwick, RS., Maglia, G., Tey, LH., & Allemann, RK. (2006). Coupling of protein motions and hydrogen transfer during catalysis by Escherichia coli dihydrofolate reductase. Biochemical Journal, 394, 259-265.


Maglia, G., & Allemanm, RK. (2003). Evidence for environmentally coupled hydrogen tunneling during dihydrofolate reductase catalysis. Journal of the American Chemical Society, 125(44), 13372-13373.
Maglia, G., Javed, MH., & Allemanm, RK. (2003). Hydride transfer during catalysis by dihydrifolate reductase from Thermotoga maritima. Biochemical Journal, 374, 529-535.


Maglia, G., & Kricka, L. J. (2001). Effect of solvents and polymers on the boronic acid enhanced peroxidase-luminol-peroxide reaction. In J. F. Case, P. J. Herring, B. H. Robinson, S. H. D. Haddock, L. J. Kricka, & P. E. Stanley (Eds.), Bioluminescence & Chemiluminescence 2000 (1 ed., Vol. 1, pp. 220-225). Singapore: World Scientific Publishing.
Last modified:08 April 2019 1.05 p.m.