Bioanalytical and Omics Techniques

Uitgebreide vaknaam	Bioanalytical and Omics Techniques
Leerdoelen	1. describe and explain the basic principles, applications, advantages and limitations of the bioanalytical and omics techniques discussed in this course 2. explain how data are analysed and interpreted in the bioanalytical and omics techniques discussed in this course 3. identify and explain the methodology and data analysis applied in omics-related scientific papers 4. describe and motivate which bioanalytical methods are suitable to address specific questions in omics-related research
Omschrijving	This course aims to provide students with a better understanding of the main principles and applications of various bio-analytical and omics techniques in life science research. Subjects covered in the course include: - General principles of genomics, transcriptomics, proteomics and metabolomics - DNA-protein interactions: EMSA, DNAse footprinting, Chip-seq, yeast2hybrid - Protein-protein interactions: FRET, complex pull down, cross-linking, cryo-EM - Secretome, subcellular protein localization, Pr-GFP studies, phenotypic heterogeneity - Separation techniques: chromatography (affinity, ion-exchange, size exclusion, HPLC), electrophoresis - Mass spectrometry: MALDI, ESI, MS-MS - Spectroscopy: CD, UV, infra-red, fluorescence spectroscopy Subjects will be explained in a number of lectures and seminars, augmented by computer tutorials. In addition, students will receive group assignments to study from selected scientific publications a specific set of techniques in more detail (find out why and how they are applied, how data are analyzed, what are the advantages and limitations) and give a presentation on their findings.
Uren per week
Onderwijsvorm	Bijeenkomst (S), Hoorcollege (LC), Opdracht (ASM), Werkcollege (T) (LC-20 hrs; S-12 hrs; T-8 hrs; ASM-10 hrs)
Toetsvorm	Presentatie (P), Schriftelijk tentamen (WE) (WE-60%; P-40% (Both grades, for the exam and presentation, need to be 5.5 or higher.))
Vaksoort	bachelor
Coördinator	dr. A.M.W.H. Thunnissen
Docent(en)	prof. dr. O.P. Kuipers ,dr. H.P. Permentier ,dr. A.M.W.H. Thunnissen
Verplichte literatuur	Titel Auteur ISBN Prijs Scientific papers, lecture slides and tutorials will be digitally provided via the Student Portal
Entreevoorwaarden	PLEASE NOTE Biology and Life Science & Technology (old curriculum) course units are only accessible for students of those degree programmes. Students from other degree programmes who would like to participate in Biology course units are obliged to contact one of the academic advisors before registration. After this contact, students have to request admission from the Board of Examiners Biology/Life Science & Technology. Failing to follow this procedure results in immediate unenrollment without prior notification.
Opmerkingen	All classes, including seminars, tutorials and student presentations, are mandatory. If a class cannot be attended, the student needs to notify teacher or course coordinator stating the reason of absence. Unexcused absences may result in exclusion from the exam. Missed assignments will have to be carried out at a later time.
Opgenomen in	Opleiding Jaar Periode Type BSc Biology: major Molecular Life Sciences 3 semester II a keuzegroep BSc LS&T: major Molecular Life Sciences (cohort 2018 and 2019) 3 semester II a keuzegroep