Practical Carrousel

Faculteit Science and Engineering
Jaar 2021/22
Vakcode WBBY048-05
Vaknaam Practical Carrousel
Niveau(s) bachelor
Voertaal Engels
Periode semester II a

Uitgebreide vaknaam Practical Carrousel
Leerdoelen At the end of the course, the student is able to:
1) perform laboratory experiments: being able to plan, carefully execute, and evaluate experiments in the area of molecular genetics, enzymology and cell biology with the help of genetic, microscopic and biochemical techniques.
2) show the ability to report on and discuss experiments: examine and interpret results with (if necessary) the help of scientific literature. Being able to critically evaluate their own work and work of others in a team.
Omschrijving Over the last decades, technological innovations in the manipulation and sequencing of DNA, protein engineering and structural biology, electrophysiology and biological spectroscopy have led to scientific breakthroughs that allowed drastic changes in medicine, agriculture and biotechnology. These innovations were based on our current understanding of the role of molecular processes in the cell and their interplay. This essential knowledge was and is continued to be created through laboratory experimentation. To understand and contribute to such developments in Molecular Life Sciences, it is essential that students are trained in modern experimental strategies and laboratory protocols. Accordingly, the Practicum Carousel allows the students to become familiar with various laboratory methods and in-silico tools that are frequently used in molecular and cell biology research. The course prepares for BSc research projects, the participation in iGEM (the genetically engineered machine competition) and (later) master research in
GBB research groups that investigate cellular and/or molecular processes in isolated or more complex biological systems. The students will be trained in planning and executing experiments that are more advanced and less routine than what is taught in the earlier courses in the curriculum. The emphasis is on learning when and how to use specific genetic and biochemical techniques. To ensure that students learn a diversity of approaches and methods, they will perform three extended experiments, each for one
week. The 1-week experiments are in the following fields:
1. enzymology and biocatalysis, with students learning to investigate and understand how to (re)design the catalytic power of
2. cell biology, with students learning experimental methods to unravel the complexity of cellular processes and regulation of
3. molecular genetics, offering students to obtain hands-on experience with modern tools in gene- or genome edititing
Uren per week
Onderwijsvorm Hoorcollege (LC), Practisch werk (PRC), Werkcollege (T)
(LC-6 hrs, T-38 hrs, PRC-70hrs, Self-study 26 hrs)
Toetsvorm Opdracht (AST), Practisch werk (PR), Schriftelijk tentamen (WE)
(WE-10%, A-40%, PR-50%. A pass (at least 6) is required for all three areas of assessment, the exam, the laboratory performance and the lab journal.)
Vaksoort bachelor
Coördinator S.K. Billerbeck, PhD.
Docent(en) P. Alvarez Sieiro, PhD. , S.K. Billerbeck, PhD. ,prof. dr. ir. M.W. Fraaije ,prof. dr. I.J. van der Klei
Verplichte literatuur
Titel Auteur ISBN Prijs
Biochemistry: Chapters "Exploring Genes" and "Exploring Proteins"
Berg et al.
Genomes 4: Theory on genetic flow (chapters 1 and 2) 4th T.A. Brown
Course manual Course coordinator and instructors
Laboratory protocols Course coordinator and instructors
Entreevoorwaarden Students should have knowledge of general biochemistry and molecular biology, including flow of genetic information, molecular genetics, and cell biochemistry. Students are expected to be familiar with the chapters "Exploring Genes" and "Exploring Proteins", in Berg et al., Biochemistry, Wiley (7th or 8th Ed.), the theory within T.A Brown's Genomes 4, Garland Science (4th Ed) and with the theoretical background of basic laboratory methods in gene cloning and analysis, enzymology, and protein analysis. Year-1 courses in the curriculum Molecular Life Sciences should have been completed.

This course unit is only accessible to students enrolled in the Bachelor's programmes Biology or old curriculum Life Science & Technology. Students following other degree programmes cannot follow this course unit and will be unenrolled in Progress.
Opmerkingen All parts are mandatory, full attendance is required.
Opgenomen in
Opleiding Jaar Periode Type
BSc Biology: major Integrative Biology  (Option A: students who follow Res. Project 1 within the majors BMS, MLS, or BN) 3 semester II a keuze
BSc Biology: major Molecular Life Sciences 2 semester II a verplicht