Cell Biology and Microscopy

Faculteit Science and Engineering
Jaar 2021/22
Vakcode WBBY034-05
Vaknaam Cell Biology and Microscopy
Niveau(s) bachelor
Voertaal Engels
Periode semester II a
Rooster rooster.rug.nl

Uitgebreide vaknaam Cell Biology and Microscopy
Leerdoelen At the end of the course, the student is able to:
1. Explain the similarities and differences between light, fluorescence, electron and atomic force microscopy
2. Describe the principles of immunofluorescence, immunolabelling, FISH, FRAP, FRET and FCS
3. Describe different types of fluorescence microscopy including wide field, confocal and superresolution microscopy,
4. Explain the mechanisms of import of mitochondrial proteins
5. Explain the molecular mechanisms of mitochondrial fission, fusion, transport and inheritance
6. Describe the process of ESCRT mediated vesicle budding from a mechanistic point of view
7. Explain how structure and content of extracellular vesicles are related to their function
8. The student is able to describe the different types of endocytosis and intracellular transport routes between ER, Golgi, plasma membrane, endosomes, lysosomes and autophagosomes.
9. The student is able to describe and explain the function and role of luminal pH, cage proteins, SNARE proteins, small GTPases, tethering factors and phosphoinositide lipids in protein sorting, organelle identity and membrane trafficking.
Omschrijving This course builds on the first year courses “Basic Cell and Molecular Biology” and “Cell biology and Immunology”. In addition, various microscopy approaches used in current molecular cell biology research will be discussed. A thorough understanding of molecular cell biology research will be obtained, focusing on mitochondria and the secretory pathway.
Examples of molecular cell biology research will be discussed from a molecular to cellular level using in vitro and in vivo approaches in yeast and mammalian model systems. For reference, parts of chapters 9 (Microscopy), 12 (Protein transport), 13 (Intracellular membrane transport) and 14 (Mitochondria and Chloroplasts) from the book Molecular Biology of the Cell will be used.
Uren per week
Onderwijsvorm Hoorcollege (LC), Werkcollege (T)
(LC: 24 hrs, T: question time-3 hrs, Self-study)
Toetsvorm Meerkeuze toets (MC), Schriftelijk tentamen (WE)
(WE: 50%, MC: 50%. Students should a) obtain a score of at least 55% for the MC (after error correction) and b) obtain a score of at least 55% for the WE)
Vaksoort bachelor
Coördinator prof. dr. I.J. van der Klei
Docent(en) prof. dr. W.H. Roos ,prof. dr. G. van den Bogaart ,prof. dr. I.J. van der Klei
Verplichte literatuur
Titel Auteur ISBN Prijs
Powerpoints of the slides (on Nestor)
Molecular Biology of the Cell (6th ed.) Alberts et al
Entreevoorwaarden This course builds on the first year courses “Basic Cell and Molecular Biology” and “Cell biology and Immunology”.

Students from other degree programmes than Biology and/or Life Science & Technology who would like to participate in this course unit are obliged to contact one of the academic advisors of the BSc. Biology before enrolling in this course in order to check whether they have meet de entrance requirements. After this contact, students also have to request admission from the Board of Examiners Biology/Life Science & Technology. Failing to follow this entire procedure before the official enrollment deadline, results in immediate unenrollment without prior notification.

Students from the Bachelor's degree programmes Biology and Life Science & Technology take priority over students from other degree programmes when the course has a maximum capacity.
Opgenomen in
Opleiding Jaar Periode Type
BSc Biology: major Integrative Biology  (Option B: students who follow Res. Project 1 within the major EE) 3 semester II a keuze
BSc Biology: major Molecular Life Sciences 2 semester II a verplicht