Lab Course

Faculteit Science and Engineering
Jaar 2021/22
Vakcode WBBY021-03
Vaknaam Lab Course
Niveau(s) propedeuse
Voertaal Engels
Periode semester I b

Uitgebreide vaknaam Lab Course
Leerdoelen At the end of the course, the student is able to:
- Safely work with (recombinant) bacteria (ML-I level)
- Grow and enrich cells on selective media
- Determine the activity of metabolic enzymes, using spectroscopic assays
- Use a phase-contrast microscope to phenotypically characterize microorganisms
- Report the experimental methods and data
- Apply theoretical concepts in microbial physiology and ecology to carry out basic microbiology experiments
Omschrijving Practical:

- SMT, including safety measures for working with microorganisms; sterile handling of cells; use of antibiotics
Cultivation of cells, including setting up of enrichment cultures; growth in liquid cultures and on solid media, determine growth
speed and doubling time in batch culture
- Microscopy and analytical methods, use of phase-contrast microscope; cell counting and serial dilutions; identification of bacteria
by phenotypic and genotypic methods
- Activity assays, including measurements of the catalytic activity of enzymes

Experiments will involve SMT & General Microbiological techniques, but will also be linked to the various majors (Molecular Life
Sciences, Ecology & Evolution and Medical Biology). Whenever possible, practical data will also be analysed by Biostatistical methods.
Uren per week
Onderwijsvorm Practisch werk (PRC)
Toetsvorm Meerkeuze toets (MC), Practisch werk (PR), Verslag (R)
(MC: SMT certificate, PRC: 2x 25%; R: 2x 25 %. In order to pass, the student needs to complete all elements with a sufficient mark (at least 5.5))
Vaksoort propedeuse
Coördinator prof. dr. A.J.M. Driessen
Docent(en) dr. E. Geuverink ,dr. J.A.K.W. Kiel , K. van der Bij, MSc. ,dr. A.U. Zielinska, PhD.
Verplichte literatuur
Titel Auteur ISBN Prijs
Brock Biology of Microorganisms 16 2021 (15th can also be used) Madigan et al.
Syllabus with protocols for the practical and instructions for lab
journal reporting and writing of the final report
A4 Lab journal
Entreevoorwaarden The practical is linked to the theoretical Microbiology course, and assumes a basic understanding of the concepts in microbial physiology and ecology taught in that course.

This course unit is only accessible to students enrolled in the Bachelor's programmes Biology or old curriculum Life Science & Technology. Students following other degree programmes cannot follow this course unit and will be unenrolled in Progress.
Opmerkingen Practical activities require that the students are present during the times allocated to the practical and that they carry out the experiments as planned. In case of absence without prior permission and without a demonstrable valid reason, the practical cannot be completed.

Students that get an insufficient grade on "Evaluation of the experimental skills" will need to redo the entire course in the next study year. Students
that fail on (one of) the other three elements are offered an opportunity to correct the(se) element(s) in the current course or the next course.

Guest lecturer: Dr P. Alvarez Sieiro

Max. capacity: 300 students.
Opgenomen in
Opleiding Jaar Periode Type
BSc Biology - Year 1 1 semester I b verplicht
BSc Biology: major Behaviour and Neurosciences 1 semester I b verplicht
BSc Biology: major Biomedical Sciences 1 semester I b verplicht
BSc Biology: major Ecology and Evolution 1 semester I b verplicht
BSc Biology: major Integrative Biology  (Semester 1) 1 semester I b verplicht
BSc Biology: major Molecular Life Sciences 1 semester I b verplicht