Biocatalysis and Green Chemistry

Uitgebreide vaknaam	Biocatalysis and Green Chemistry
Leerdoelen	At the end of the course, the student is able to: 1. demonstrate knowledge of the diversity of reactions catalyzed by enzymes, including the catalytic properties of serine hydrolases, flavoenzymes, flavoenzymes, and other groups of enzymes included in the course syllabus 2. explain the catalytic mechanism of important enzymes, including acid-base catalysis, covalent catalysis and the role of several common cofactors 3. explain the use of enzymes for catalyzing important reactions such as hydrolysis, group transfer, oxidation, and conjugation and application in different biocatalytic conversions, such as production of enantiopure compounds and biomass processing 4. evaluate, explain and critically comment on the relations between enzyme properties, catalytic mechanisms, reaction types, and application in different biocatalytic conversions of potential or realized practical relevance 5. present in a coherent and convincing way, and describe in a written report, scientific approaches that can be used to develop a new biocatalytic process, including the design of a project outline or proposal with a realistic well-described work plan.
Omschrijving	Catalysis is the key technology in the development of green processes for synthetic chemistry. Enzymes are versatile and often highly selective catalysts that operate under mild conditions in water. The possibility to use enzymes in synthetic chemistry is attractive because of their wide reaction scope and the availability of methods to tailor enzyme properties for specific conversions. This course aims to provide an understanding of enzyme-catalyzed reactions, including mechanisms of important enzymes, the basis and analysis of chemo-, regio- and stereoselectivity of enzymes, and the relationship between enzyme properties and their application in industrial biocatalytic processes. Various classes of enzymes are discussed, such as hydrolases, oxygenases, and C-C bond forming enzymes. Except for their catalytic properties, also methods for engineering and applying enzymes are discussed. Topics in this course are: • biocatalysis: regio- and stereoselectivity of enzymes; chemoselectivity, enzyme kinetics, mechanisms • technical aspects of biocatalysis (incl. enzyme production, immobilization, use of solvents) • approaches of enzyme discovery and engineering • role and chemical versatility of enzyme cofactors • hydrolases (such as lipases and proteases) in biocatalysis: mechanism, hydrolysis, synthesis, kinetic description • redox enzymes, incl. nicotinamide, flavin, heme, non-heme iron cofactor dependent enzymes, and other biocatalytically relevant enzymes • biocatalyst development and implementation of new processes.
Uren per week
Onderwijsvorm	Hoorcollege (LC), Opdracht (ASM), Werkcollege (T) (Workload: Self study 112 hrs, Lecture 16 hrs, Tutorial (exercises) 8 hrs, Tutorial (proposal assignments) 4 hrs, Assignment 4 hrs)
Toetsvorm	Presentatie (P), Schriftelijk tentamen (WE), Verslag (R) (Final mark: 50% written exam and 50% report. See remarks.)
Vaksoort	master
Coördinator	prof. dr. ir. M.W. Fraaije
Docent(en)	prof. dr. ir. M.W. Fraaije ,Prof. Dr. K.U. Loos
Verplichte literatuur	Titel Auteur ISBN Prijs syllabus with description of content of the course, available via Nestor review papers, supplied with the syllabus and available via Nestor copies of sheets, available via Nestor tutorial questions. handed out during tutorial sessions
Entreevoorwaarden	The course unit requires prior knowledge of biochemistry, molecular biology, organic chemistry, and catalysis, all studied in the first or second years of the BSc program Chemistry (tracks Life or Sustainability, both are fine) or Biology (track Molecular Biology).
Opmerkingen	The final grade is calculated as follows: 50% written exam and 50% assignment (25% presentation, 25% report). The written exam and the assignment each require a pass (6 or higher) to receive a final pass and grade.
Opgenomen in	Opleiding Jaar Periode Type MSc Biology: Research  (Compulsory master courses) - semester I b keuze MSc Biology: Science, Business and Policy  (Compulsory master courses) - semester I b keuze MSc Biomolecular Sciences  (Electives/Optional modules) - semester I b keuze MSc Chemistry  (Courses available for specializations) - semester I b verplicht MSc Chemistry: Science, Business and Policy  (Electives) - semester I b keuze MSc Courses for Exchange Students: Chemistry - Chemical Engineering - semester I b MSc Ecology and Evolution: Ecology and Conservation  (Electives/optional modules) - semester I b keuze MSc Ecology and Evolution: Evolutionary Biology  (Electives/optional modules) - semester I b keuze MSc Energy and Environmental Sciences  (Electives) 1 semester I b keuze MSc Marine Biology: Research  (Electives/Optional modules) - semester I b keuze