Cell Biology and Microscopy

Faculteit Science and Engineering
Jaar 2019/20
Vakcode WBLS19020
Vaknaam Cell Biology and Microscopy
Niveau(s) bachelor
Voertaal Engels
Periode semester II a
Rooster rooster.rug.nl

Uitgebreide vaknaam Cell Biology and Microscopy
Leerdoelen 1. Explain the similarities and differences between light, fluorescence, electron and atomic force microscopy
2. Describe the principles of immunofluorescence, immunolabelling, FISH, FRAP, FRET and FCS
3. Describe different types of fluorescence microscopy including wide field, confocal and superresolution microscopy,
4. Explain the mechanisms of import of a mitochondrial matrix and innermembrane protein
5. explain the molecular mechanisms of mitochondrial fission, fusion, transport and inheritance
6. Describe the process of ESCRT mediated vesicle budding from a mechanistic point of view
7. Explain how structure and content of extracellular vesicles are related to their function
8. The student is able to describe the different types of endocytosis and intracellular transport routes between ER, Golgi, plasma membrane, endosomes, lysosomes and autophagosomes.
9. The student is able to describe and explain the function and role of luminal pH, cage proteins, SNARE proteins, small GTPases, tethering factors and phosphoinositide lipids in protein sorting, organelle identity and membrane trafficking.
Omschrijving Various microscopy approaches used in current molecular cell biology research will be discussed. A thorough understanding of molecular cell biology research will be obtained, focusing on mitochondria and the secretory pathway. Examples of molecular cell biology research will be discussed from a molecular to cellular level using in vitro and in vivo approaches in yeast and mammalian model systems.

The students will be introduced to modern microscopy techniques used in the molecular life sciences (from the protein to cellular level). Topics include electron and fluorescence microscopy of cells/tissues, the use of fluorescent dyes and proteins, special fluorescence approaches such as FRAP (fluorescence recovery after photobleaching), FCS (fluorescence correlation spectroscopy) and FRET (Förster resonance energy transfer), superresolution microscopy, atomic force microscopy and optical tweezers.

An overview will be provided of the molecular mechanisms involved in mitochondrial biogenesis and dynamics (more specifically: protein translocation, fission (Dynamin related proteins) and fusion, inheritance and transport, contact sites). The biochemical, genetic and microscopy approaches used to study mitochondria will be discussed.

Protein secretion:
Intracellular membrane trafficking will be discussed, including ER-Golgi transport, exocytosis, endocytosis and lysosomal trafficking. The students will be introduced to the key mechanisms of intracellular cargo sorting, the formation of trafficking vesicles, the docking and fusion to target organelles and the molecular determinants of organellar identity.

ESCRT System and extracellular vesicles:
Many cellular processes such as endosomal vesicle budding, virus budding, and cytokinesis require extensive membrane remodeling by the endosomal sorting complex required for transport (ESCRT), of which a mechanistic view will be presented in this course. In addition an overview on extracellular vesicles, which are for instance involved in cell-to-cell communication and disease, will be discussed from a structure/function point of view.
Uren per week
Onderwijsvorm Hoorcollege (LC), Werkcollege (T)
(LC: 24 hrs, T: question time-3 hrs, Self-study)
Toetsvorm Meerkeuze toets (MC), Schriftelijk tentamen (WE)
(WE: 50%, MC: 50%. Students should a) obtain a score of at least 55% for the MC and b) obtain a score of at least 55% for the WE)
Vaksoort bachelor
Coördinator prof. dr. I.J. van der Klei
Docent(en) prof. dr. W.H. Roos ,prof. dr. G. van den Bogaart ,prof. dr. I.J. van der Klei
Verplichte literatuur
Titel Auteur ISBN Prijs
Molecular Biology of the Cell (6th ed.) Alberts et al
Powerpoints of the slides (on Nestor)
Entreevoorwaarden This course builds on the first year courses “Basic Cell and Molecular Biology” and “Cell biology and Immunology”.
Opmerkingen For reference, parts of chapters 9 (Microscopy), 12 (Protein transport), 13 (Intracellular membrane transport) and 14 (Mitochondria and Chloroplasts) from the book Molecular Biology of the Cell will be used.
Opgenomen in
Opleiding Jaar Periode Type
BSc Biology: major Molecular Life Sciences 2 semester II a verplicht
BSc LS&T: major Molecular Life Sciences (cohort 2018 and 2019) 2 semester II a verplicht