Electron Microscopy of Biological Macromolecules

Faculteit Science and Engineering
Jaar 2019/20
Vakcode MLGBB03
Vaknaam Electron Microscopy of Biological Macromolecules
Niveau(s) master
Voertaal Engels
Periode semester I b (11-11-2019 till 30-11-2019)
Rooster rooster.rug.nl

Uitgebreide vaknaam Electron Microscopy of Biological Macromolecules
Leerdoelen At the end of the course, the student is able to:
1) describe the physical background of electron microscopy (EM)
2) explain the basics of contrast and image formation
3) operate an electron microscope (in a basic mode)
4) prepare specimens by negative staining.
5) interpret images of negatively stained and cryo-EM specimens
6) carry out basic image processing of high-resolution cryo-EM data
7) understand the possibilities and limitations of EM for structural research in different disciplines
Omschrijving This 3-week course is an introduction to electron microscopy (EM) and is meant for students with an interest in structures of biological macromolecules such as proteins. The focus is on learning how to use a transmission electron microscope, including EM sample preparation (2-weeks). In the last week, students will learn state-of-the art computer image processing of protein structures (1 week). Students are expected to obtain a better understanding of the advantages and disadvantages, the possibilities and limitation of the technique and were it is employed. Recent breakthroughs in cryo-electron microscopy (cryo-EM) have revolutionize the field entirely making it often the method of choice for high resolution structure determination of proteins, which was acknowledged by the Nobel Prize in Chemistry in 2017.
Uren per week
Onderwijsvorm Hoorcollege (LC), Practisch werk (PRC)
(Practical, lectures, demo)
Toetsvorm Opdracht (AST), Practisch werk (PR), Schriftelijk tentamen (WE)
(Final mark = O.5 Written exam + 0.3 Practical + O.2 Assignment, Exam grade needs to be higher than 5.5)
Vaksoort master
Coördinator C. Batista Paulino, PhD.
Docent(en) C. Batista Paulino, PhD. ,dr. M.C.A. Stuart
Verplichte literatuur
Titel Auteur ISBN Prijs
Course manual
Entreevoorwaarden The course unit assumes a basic understanding of physics, protein structure and other structure biology technique (mostly high school level).
Opmerkingen Lectures and practice are mandatory

Capacity: 16 students max.
Opgenomen in
Opleiding Jaar Periode Type
MSc Biology: Research  (Compulsory master courses) - semester I b keuze
MSc Biology: Science, Business and Policy  (Compulsory master courses) - semester I b keuze
MSc Biomolecular Sciences  (Electives/Optional modules) - semester I b keuze