Advanced Protein Crystallography
Faculteit | Science and Engineering |
Jaar | 2019/20 |
Vakcode | MLBB007 |
Vaknaam | Advanced Protein Crystallography |
Niveau(s) | master |
Voertaal | Engels |
Periode | semester I b (02-12-2019 till 21-12-219) |
ECTS | 5 |
Rooster | rooster.rug.nl |
Uitgebreide vaknaam | Advanced Protein Crystallography | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Leerdoelen | after the course the student is able to: 1) explain the main fundamentals and practices of protein crystallography; 2) perform basic calculations relevant to crystallography (e.g. Matthews number, solvent content, structure factors given atomic positions and the crystal symmetry, electron density distribution given structure factors and unit cell dimensions); 3) explain the principles of point and space groups, and apply these principles to crystallographic problems; 4) apply statistical methods to protein crystallography; 5) describe methods how to derive crystallographic phases; 6) obtain and complete a structural model of a protein starting from diffraction data; 7) assess the quality of a diffraction data set and the associated protein structure, and interpret and judge the statistics of a given crystal structure determination; |
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Omschrijving | This course will provide insights on the important fundamentals and practices of protein X-ray crystallography. The aims of the course are to learn how protein crystal structures are determined, and to acquire the knowledge and skills necessary for critically analysing a 3D crystal structure. Subjects discussed during the course are: • Protein crystallization and crystal geometry; • Basic diffraction theory; • X-ray instrumentation; • Strategies for phase determination; • Basics of model building and refinement; • Structure validation: quality assessment of a protein crystal structure. Lecture classes are combined with discussion/exercise sessions: students will obtain reading assignments, will have to answer exercises, and are requested to prepare questions and subjects for group discussions. The lecture/discussion/exercise course is complemented with a workshop on the use of the various protein crystallographic computer programs. |
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Uren per week | |||||||||||||||||||||||||||||||||||||||||||||||||||||
Onderwijsvorm |
Hoorcollege (LC), Practisch werk (PRC)
(14 hours lectures, 7 hours tutorial, 20 hours computer-practicals) |
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Toetsvorm |
Practisch werk (PR), Schriftelijk tentamen (WE)
(Written exam with essay questions (Effort test: students should submit a short report of the tutorials/computer practical. This report is examined and used for feedback, but not graded.)) |
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Vaksoort | master | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Coördinator | dr. A.M.W.H. Thunnissen | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Docent(en) | dr. A. Guskov ,dr. A.M.W.H. Thunnissen | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Verplichte literatuur |
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Entreevoorwaarden | Students enrolled in Biomolecular Sciences need to pre subscribe via esc-lifesciences.zernike@rug.nl in June The course unit assumes prior knowledge acquired from relevant courses in Bachelor’s programmes in Chemistry and/or in Biology, covering the following subjects: fundamentals of protein structure, mathematics (basic calculus and vectors), biophysical chemistry. Relevant courses in the BSc Chemistry and BSc Biology or LS&T programmes at the University of Groningen: Calculus I (WICAL1-12), Biochemistry (CHBC-10), Recombinant DNA and Biotechnology (CHRDNAB-11), Cell physiology: Energy and Structure (WLP10B18), Structural Biology (WLP10B22), Mathematics for Life Sciences (WLP10B22). |
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Opmerkingen | Maximum capacity is 24 students. Priority will be given to students who need to do this course for the master programme Biomolecular Sciences or the specialization Chemical Biology. To subscribe/enroll please contact esc-lifesciences.zernike@rug.nl Location: Research group Protein Crystallography (GBB), Life Sciences Building (Room 5171.0060/0056), Nijenborgh 7, Groningen. |
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Opgenomen in |
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