Advanced Protein Crystallography

Uitgebreide vaknaam	Advanced Protein Crystallography
Leerdoelen	after the course the student is able to: 1) explain the main fundamentals and practices of protein crystallography; 2) perform basic calculations relevant to crystallography (e.g. Matthews number, solvent content, structure factors given atomic positions and the crystal symmetry, electron density distribution given structure factors and unit cell dimensions); 3) explain the principles of point and space groups, and apply these principles to crystallographic problems; 4) apply statistical methods to protein crystallography; 5) describe methods how to derive crystallographic phases; 6) obtain and complete a structural model of a protein starting from diffraction data; 7) assess the quality of a diffraction data set and the associated protein structure, and interpret and judge the statistics of a given crystal structure determination;
Omschrijving	This course will provide insights on the important fundamentals and practices of protein X-ray crystallography. The aims of the course are to learn how protein crystal structures are determined, and to acquire the knowledge and skills necessary for critically analysing a 3D crystal structure. Subjects discussed during the course are: • Protein crystallization and crystal geometry; • Basic diffraction theory; • X-ray instrumentation; • Strategies for phase determination; • Basics of model building and refinement; • Structure validation: quality assessment of a protein crystal structure. Lecture classes are combined with discussion/exercise sessions: students will obtain reading assignments, will have to answer exercises, and are requested to prepare questions and subjects for group discussions. The lecture/discussion/exercise course is complemented with a workshop on the use of the various protein crystallographic computer programs.
Uren per week
Onderwijsvorm	Hoorcollege (LC), Practisch werk (PRC) (14 hours lectures, 7 hours tutorial, 20 hours computer-practicals)
Toetsvorm	Practisch werk (PR), Schriftelijk tentamen (WE) (Written exam with essay questions (Effort test: students should submit a short report of the tutorials/computer practical. This report is examined and used for feedback, but not graded.))
Vaksoort	master
Coördinator	dr. A.M.W.H. Thunnissen
Docent(en)	dr. A. Guskov ,dr. A.M.W.H. Thunnissen
Verplichte literatuur	Titel Auteur ISBN Prijs Advised literature: Biomolecular Crystallography: Principles, Practice and Application to Structural Biology, Garland Science, Taylor & Francis Group. accessible via UG Library Bernhard Rupp 978-0-8153-4081-2 ca. €  86,00 Advised literature: Principles of Protein X-Ray Crystallography, 3rd 2007. available as an e-book via SmartCat Jan Drenth ISBN 0-8153-4081-8 ca. €  48,00 Mandatory literature: Reader containing lecture slides, exercises, computer practical manual, tutorials and copies of relevant scientific articles
Entreevoorwaarden	Students enrolled in Biomolecular Sciences need to pre subscribe via esc-lifesciences.zernike@rug.nl in June The course unit assumes prior knowledge acquired from relevant courses in Bachelor’s programmes in Chemistry and/or in Biology, covering the following subjects: fundamentals of protein structure, mathematics (basic calculus and vectors), biophysical chemistry. Relevant courses in the BSc Chemistry and BSc Biology or LS&T programmes at the University of Groningen: Calculus I (WICAL1-12), Biochemistry (CHBC-10), Recombinant DNA and Biotechnology (CHRDNAB-11), Cell physiology: Energy and Structure (WLP10B18), Structural Biology (WLP10B22), Mathematics for Life Sciences (WLP10B22).
Opmerkingen	Maximum capacity is 24 students. Priority will be given to students who need to do this course for the master programme Biomolecular Sciences or the specialization Chemical Biology. To subscribe/enroll please contact esc-lifesciences.zernike@rug.nl Location: Research group Protein Crystallography (GBB), Life Sciences Building (Room 5171.0060/0056), Nijenborgh 7, Groningen.
Opgenomen in	Opleiding Jaar Periode Type MSc Biology: Research  (Compulsory master courses) - semester I b keuze MSc Biology: Science, Business and Policy  (Compulsory master courses) - semester I b keuze MSc Biomolecular Sciences  ( Compulsory courses Chemical Biology) - semester I b verplicht MSc Biomolecular Sciences  ( Compulsory courses general programme (3 out of 9)) - semester I b keuzegroep MSc Biomolecular Sciences  (Electives/Optional modules) - semester I b keuze MSc Chemistry: Advanced Materials  (Electives) - semester I b keuze MSc Chemistry: Catalysis and Green Chemistry  (Electives) - semester I b keuze MSc Chemistry: Chemical Biology  ( Core Programme) - semester I b verplicht MSc Chemistry: Chemical Biology  (Electives) - semester I b keuze MSc Chemistry: Erasmus Mundus Theoretical Chemistry and Computing Modelling  (Electives) - semester I b keuze MSc Chemistry: Science, Business and Policy  (Electives) - semester I b keuze MSc Nanoscience  (Optional Courses) - semester I b keuze