Advanced Genetic Engineering

Faculteit | Science and Engineering |
Jaar | 2022/23 |
Vakcode | WMBS006-05 |
Vaknaam | Advanced Genetic Engineering |
Niveau(s) | master |
Voertaal | Engels |
Periode | semester I b (9-01-2023 till 29-01-2023) |
ECTS | 5 |
Rooster | rooster.rug.nl |
Uitgebreide vaknaam | Advanced genetic engineering and complex gene regulatory circuitries | ||||||||||||||||||||||||||||||||
Leerdoelen | At the end of the course, the student is able to: 1. Explain the underlying principles of Synthetic Biology (e.g what are the scientific approaches, in which way is it different than conventional genetic engineering?) 2. Describe the principles of genome editing and describe the advantages and disadvantages of techniques such as CRISPR-cas, and describe its application in molecular biology. 3. Design and build a genetic circuit using biobricks and experimentally prove its functionality. 4. Analyse a scientifc, relevant article using the information presented during the lectures, and present this analysis clearly tot their peers. 5. Identify ethical and societal problems of synthetic biology and evaluate pros and cons of its approach. |
||||||||||||||||||||||||||||||||
Omschrijving | The emphasis will be on the practical aspects of genetic engineering of novel circuitries and on the principles of Synthetic Biology, both in silico and in the lab. In principle, the students will work in pairs on a research project in the practical part. The students will avail of- and study a number of bacterial strains with earlier developed DNA-biobricks (also from iGEM collections). Novel circuitries will be designed and engineered using Escherichia coli as hosts. The engineered circuits will be characterized and functionality will be tested experimentally (e.g. by fluorescence microscopy or enzymatic assays). In this course, theory and laboratory experiments will be combined to give an in-depth view of current (highthroughput) genetic engineering approaches such as CRISPR based systems, gene regulatory mechanisms and -networks related to microbial physiology-Advanced genetic engineering: this includes use of Biobricks, Gibson assembly, CRISPR-based genetic systems, expansion of the genetic code, use of BACs and YACs, toolboxes, gene integration, complex genetic circuitries (natural and synthetic). |
||||||||||||||||||||||||||||||||
Uren per week | |||||||||||||||||||||||||||||||||
Onderwijsvorm | Hoorcollege (LC), Opdracht (ASM), Practisch werk (PRC), Werkcollege (T) | ||||||||||||||||||||||||||||||||
Toetsvorm |
Practisch werk (PR), Presentatie (P), Schriftelijk tentamen (WE)
(For grading of the presentation(s) the FWN rubric is used. Students have to perform the HWO on ethical and societal problems, and actively take part in the discussion. A grade of 5.5 has to be minimally attained on all three elements.) |
||||||||||||||||||||||||||||||||
Vaksoort | master | ||||||||||||||||||||||||||||||||
Coördinator | Prof. Dr. T.E.F. Quax | ||||||||||||||||||||||||||||||||
Docent(en) | and others , H. Karssens , B.P. Kuiper, MSc. ,Prof. Dr. T.E.F. Quax | ||||||||||||||||||||||||||||||||
Verplichte literatuur |
|
||||||||||||||||||||||||||||||||
Entreevoorwaarden | The course unit assumes a good background in microbiology and genetics courses as presented in the bachelor programme Moleculaire levenswetenschappen. | ||||||||||||||||||||||||||||||||
Opmerkingen | Students from the degree programme Biomolecular Sciences have priority, to register please send an email to: academicadvisor.mscbio@rug.nl Maximum capacity: 18 students |
||||||||||||||||||||||||||||||||
Opgenomen in |
|