Advanced Genetic Engineering

Uitgebreide vaknaam	Advanced genetic engineering and complex gene regulatory circuitries
Leerdoelen	At the end of the course, the student is able to: 1. Explain the underlying principles of Synthetic Biology (e.g what are the scientific approaches, in which way is it different than conventional genetic engineering?) 2. Describe the principles of genome editing and describe the advantages and disadvantages of techniques such as CRISPR-cas, and describe its application in molecular biology. 3. Design and build a genetic circuit using biobricks and experimentally prove its functionality. 4. Analyse a scientifc, relevant article using the information presented during the lectures, and present this analysis clearly tot their peers. 5. Identify ethical and societal problems of synthetic biology and evaluate pros and cons of its approach.
Omschrijving	The emphasis will be on the practical aspects of genetic engineering of novel circuitries and on the principles of Synthetic Biology, both in silico and in the lab. In principle, the students will work in pairs on a research project in the practical part. The students will avail of- and study a number of bacterial strains with earlier developed DNA-biobricks (also from iGEM collections). Novel circuitries will be designed and engineered using Escherichia coli as hosts. The engineered circuits will be characterized and functionality will be tested experimentally (e.g. by fluorescence microscopy or enzymatic assays). In this course, theory and laboratory experiments will be combined to give an in-depth view of current (highthroughput) genetic engineering approaches such as CRISPR based systems, gene regulatory mechanisms and -networks related to microbial physiology-Advanced genetic engineering: this includes use of Biobricks, Gibson assembly, CRISPR-based genetic systems, expansion of the genetic code, use of BACs and YACs, toolboxes, gene integration, complex genetic circuitries (natural and synthetic).
Uren per week
Onderwijsvorm	Hoorcollege (LC), Opdracht (ASM), Practisch werk (PRC), Werkcollege (T)
Toetsvorm	Practisch werk (PR), Presentatie (P), Schriftelijk tentamen (WE) (For grading of the presentation(s) the FWN rubric is used. Students have to perform the HWO on ethical and societal problems, and actively take part in the discussion. A grade of 5.5 has to be minimally attained on all three elements.)
Vaksoort	master
Coördinator	Prof. Dr. T.E.F. Quax
Docent(en)	and others , H. Karssens , B.P. Kuiper, MSc. ,Prof. Dr. T.E.F. Quax
Verplichte literatuur	Titel Auteur ISBN Prijs Scientific articles syllabus
Entreevoorwaarden	The course unit assumes a good background in microbiology and genetics courses as presented in the bachelor programme Moleculaire levenswetenschappen.
Opmerkingen	Students from the degree programme Biomolecular Sciences have priority, to register please send an email to: academicadvisor.mscbio@rug.nl Maximum capacity: 18 students
Opgenomen in	Opleiding Jaar Periode Type MSc Biology: Research  (Compulsory master courses) - semester I b keuze MSc Biology: Science, Business and Policy  (Compulsory master courses) - semester I b keuze MSc Biomolecular Sciences  (Electives/Optional modules) - semester I b keuze MSc Biomolecular Sciences  ( Compulsory courses general programme (3 out of 9)) - semester I b keuzegroep MSc Ecology and Evolution: Ecology and Conservation  (Electives/optional modules) - semester I b keuze MSc Ecology and Evolution: Evolutionary Biology  (Electives/optional modules) - semester I b keuze MSc Marine Biology: Research  (Electives/Optional modules) - semester I b keuze