CRISPR editing

Uitgebreide vaknaam	Editing, regulating and targeting genomes with CRISPR-Cas9
Leerdoelen	At the end of the course, the student is able to: 1) Describe the history and applications of genome editing through CRISPR-Cas9 2) Explain data derived from and concepts behind CRISPR-based genome editing experiments 3) Construct a CRISPR-based genome editing strategy 4) Present a CRISPR-based genome editing project 5) Apply CRISPR in the lab in your own project
Omschrijving	The fourth edition of this course will start with a maximum of 30 students in the Academic year 2020-2021. A high level of involvement, initiative and creativity is being expected from the participating students. The theoretical basis and the application of gene editing through CRISPR-Cas9 will be taught in a series of 6 lectures (4 hours each, including a 1 hour 'application' lecture by a guest lecturer). Lectures are complemented by literature discussion, theoretical and practical assignments, and laboratory training. CRISPR-Cas9 was discovered as an immune system in prokaryotes and archaea that confers resistance to bacteriophages and invading nucleic acids by providing a form of acquired immunity. Clustered regularly interspaced short palindromic repeats (CRISPR) are segments of prokaryotic DNA containing short repetitions of base sequences. Each repetition is followed by short segments of "spacer DNA" from previous exposures to foreign DNA. CRISPR-associated (Cas) proteins use the CRISPR spacers to recognize, cut and thereby remove invading (exogenous) genetic elements in a manner similar to RNA interference in eukaryotic organisms. CRISPR-Cas9 provides biomedical research with a cut-and-paste toolbox for genome editing in mammalian cells. Allowing scientist to cure genetic diseases by repairing the disease causing mutation. The technology is rapidly changing biomedical science and is expected to form the basis of personalised medicine in the future. This course is targeted for students that have the ambition or are planning to use CRISPR technology during one of their research internships and will start with the basics of CRISPR engineering. Note that the course therefore is less suited for students that already have (ample) experience with CRISPR engineering. For such students who still want to get more experience, we recommend to apply for an internship at the CRISPR center (1-2 placements per year available, so apply early with a clear motivation).
Uren per week
Onderwijsvorm	Hoorcollege (LC), Opdracht (ASM), Practisch werk (PRC), Werkcollege (T) (L: 24 hours, T: 10 hours, ASM: 2*16 hours, PRC: 12 hours, Self study: 92 hours. Students need to attend all the lectures, seminars and practical lab training in order to pass the course.)
Toetsvorm	Practisch werk (PR), Presentatie (P), Schriftelijk tentamen (WE), Verslag (R) (For each assignment a mark will be given between 0 and 10. To pass the course each individual assignment must be rewarded with a 6 or higher. The final mark will be calculated as follows: • Written test x 0.5 (50%) • Group assignment x 0.2 (20%) • Report practical training x 0.15 (15%) • Presentation x 0.2 (15%) • Group assignment CRISPR design (pass or fail) Mandatory presence: Yes , Students need to attend all the lectures, seminars and practical lab training in order to pass the course.)
Vaksoort	master
Coördinator	prof. dr. ir. F. Foijer
Docent(en)	ing. M.J.C. Broekhuis ,prof. dr. ir. F. Foijer , J. Seiler
Verplichte literatuur	Titel Auteur ISBN Prijs No required materials
Entreevoorwaarden	BSc in biomedical sciences (or equivalent)
Opmerkingen	Max. capacity: 30 students. This is a MSc. Biomedical Sciences course unit: see student portal for priority regulations in place. This course was registered last year with course code WMBM17003
Opgenomen in	Opleiding Jaar Periode Type MSc Biomedical Sciences: Biology of Ageing  ( Master Courses) - semester I a keuzegroep MSc Biomedical Sciences: Biology of Cancer and Immune System  ( Masters Courses) - semester I a keuzegroep MSc Biomedical Sciences: Biology of Food and Nutrition  (Electives (10 ECTS)) - semester I a keuze MSc Biomedical Sciences: Neuroscience  (Electives (10 ECTS)) - semester I a keuze MSc Biomedical Sciences: Research  (Electives) - semester I a keuze MSc Biomedical Sciences: Science, Business and Policy  (Electives (10 ECTS)) - semester I a keuze