Recombinant DNA and Biotechnology

Faculteit Science and Engineering
Jaar 2022/23
Vakcode WBCH042-05
Vaknaam Recombinant DNA and Biotechnology
Niveau(s) bachelor
Voertaal Engels
Periode semester II b
ECTS 5
Rooster rooster.rug.nl

Uitgebreide vaknaam Recombinant DNA and Biotechnology
Leerdoelen At the end of the course, the student is able to:
1.
describe the backgrounds of molecular biological concepts (central dogma).
2.
describe the basic concepts of, and the role of, biomolecules in transcription, translation, and transcriptional regulation in prokaryotes and eukaryotes
3.
discuss and interpret results of recombinant DNA and proteomic experiments, and are able to compare the different techniques
4.
design experimental strategies to solve molecular biological questions and make new discoveries.
5.
use internet databases containing biological data to analyze DNA and protein sequences
6.
describe basic applications of these techniques in biotechnology, forensics and medicine
7.
describe genetic causes of several diseases
Omschrijving Genome-centered molecular biology and protein biochemistry are rapidly evolving fields with great impact on human life, because it directly influences medicine, biotechnology and forensics.

The course gives an introduction to the fundamental concepts of replication, transcription, translation and the regulation of transcription. The genetics of bacteria and yeasts are discussed and their application in recombinant DNA techniques and molecular biology. Topics include all aspects of cloning, reporter assays, PCR, immunochemistry, RNA interference, CRISPR, chromosomal recombination, gene mapping and transgenic mice. The course introduces genomics, the Human Genome Project, a description of bioinformatics and experimental techniques for large scale genomic studies. In addition to the core molecular biological techniques, a number of protein-centered techniques will be presented, including immunoprecipitation, gel electrophoresis and mass spectrometry, which are used in proteomics.

These techniques help us to study fundamental concepts such as gene function, regulation, protein-protein interaction, and signal transduction. The students will become familiar with the analysis of disease causing genes, production of protein drugs such as insulin and therapeutic monoclonal antibodies, and DNA fingerprinting and forensics. Examples of practical applications in human medicine, such as gene therapy, will be discussed, as well as the future of human genetics and genomics projects.
Uren per week
Onderwijsvorm Hoorcollege (LC), Opdracht (ASM), Practisch werk (PRC), Werkcollege (T)
(Total hours of lectures: 26 hours, tutorials: 3 hours, computer lab: 3 hours, self study: 110 hours)
Toetsvorm Meerkeuze toets (MC), Opdracht (AST), Practisch werk (PR), Schriftelijk tentamen (WE)
(See remarks.)
Vaksoort bachelor
Coördinator dr. M.H.K. Linskens
Docent(en) dr. M.H.K. Linskens ,prof. dr. D.J. Slotboom
Verplichte literatuur
Titel Auteur ISBN Prijs
Scientific papers (pdf's supplied via Nestor during course)
(Parts of) Biochemistry Chapters 4, 5, 6, 28, 29 30, 31, 32 Berg, Tymoczko, Gatto and Stryer published by Freeman & Macmillan 9781464126109
Relevant chapters will be provided from the book Gene Cloning & DNA Analysis (2016)
available online in the RUG library (via SmartCat)
Brown, T.A
Entreevoorwaarden The course unit assumes prior knowledge acquired from Biochemistry (BSc Chemistry or Biology).

The course unit is preceded by the course Cellular Chemistry in the degree programme Chemistry. The knowledge from both courses prepares students for the third year courses and research project in the track Chemistry of Life.
Opmerkingen ssessment is based on 4 criteria (multiple choice, computer lab, assignments, written exam).
Multiple choice maximally 20 points
Computer lab maximally 15 points
Literature assignment maximally 15 points
Mass Spec assignment 10 points
Experimental design assignment 20 points
Written exam 20 points
The total score has to be at least 55 points to pass.

Mandatory:
First lecture: mandatory, needed to explain the structure of the course and the grading protocol.
Computer lab: attendance and participation required, handing in of assignment
Tutorial: attendance and participation required, handing in of assignment
Opgenomen in
Opleiding Jaar Periode Type
BSc Chemistry  ( Chemistry of Life) 2 semester II b verplicht
BSc Courses for Exchange Students: Chemistry - Chemical Engineering - semester II b