Cellular Chemistry

Faculteit Science and Engineering
Jaar 2022/23
Vakcode WBCH021-05
Vaknaam Cellular Chemistry
Niveau(s) bachelor
Voertaal Engels
Periode semester II a
ECTS 5
Rooster rooster.rug.nl

Uitgebreide vaknaam Cellular Chemistry
Leerdoelen At the end of the course, the student is able to:
1.
describe and explain the dimensions of cells and components in cells and to describe the rates of a number of cellular processes.
2.

describe and explain the basic functioning of a modern light microscope, the principles of advanced fluorescence microscopy techniques and their applications in studying cellular structures and protein dynamics.
3.
describe and explain the underlying principles and applications of flow cytometry and protein mass spectrometry in studying cell signaling events.
4.
describe and explain the processes of the cell cycle and its regulation.
5.
describe and explain the mechanisms of protein transport into organelles & general principles of cell signalling.
6.
describe and explain the building blocks of the cytoskeleton and their functions.
7.
describe and explain the origin and consequences of macro molecular crowding.
8.
analyse the literature and identify how advanced fluorescence microscopy, proteomics or flow cytometry is used to study cellular structures, protein dynamics and cell signaling events.
Omschrijving This course aims to provide insight into the field of cell biology from a chemical and quantitative perspective. Specifically, students will acquire solid knowledge about the important dynamic and spatial processes in cells listed below and will learn about experimental methods and how to apply these to study cellular processes.

Topics
- Overall construction plans for cells: numbers, dimensions and architecture of cells
- Light microscopy as a technique to study spatial and dynamic processes in cells; photochemistry of fluorescent proteins
- Theme ‘space’: incl. (i) intracellular compartments and protein sorting, (ii) intracellular vesicular traffic, (iii) cell communication, (iv) cytoskeleton and self-assembly
- Theme ‘time’: incl. (i) cell cycle and (ii) stochastic effects in cells

Because it is important to understand how fluorescence microscopy, flow cytometry and mass spectrometry techniques are applied for the tracking of processes and molecules in a cell, each student will study a research article related to the application of one of these techniques. The outcome of the study (how the technique enables to study one of the cellular processes as indicated in the themes) will be assessed by an oral presentation to peers.
Uren per week
Onderwijsvorm Hoorcollege (LC), Opdracht (ASM), Werkcollege (T)
(Workload: Self study 112 hrs, Lecture 12 hrs, Tutorial 6 hrs)
Toetsvorm Presentatie (P), Schriftelijk tentamen (WE)
(pass mark: see remarks)
Vaksoort bachelor
Coördinator F. Bianchi, PhD.
Docent(en) F. Bianchi, PhD. ,Dr. J.L. Kamenz
Verplichte literatuur
Titel Auteur ISBN Prijs
Chapters from:
Molecular Biology of the Cell, 6th edition, Alberts et al
Chapters 9, 12, 13, 15, 16, 17, 18, 20
Chapters from:
Physical Biology of the Cell, 2nd edition, Phillips et al
Chapters 13, 14
Entreevoorwaarden The course unit assumes prior knowledge from:
Biochemistry year 1, including practice course
Recombinant DNA and Biotechnology
Bioenergetics and Metabolism
Chemical Biology
(Bio)Catalysis

The chemistry students have all the basics to follow this course (biochemistry incl practicum, DNA, bioenergetics and metabolism). The bio-knowledge of the physics students is less broad at this stage, but this is not considered a problem.
Opmerkingen The result of the written exam (80%) combined with the assessment of the presentation (20%) determines the mark of this course. The written exam is an open question-closed book exam. The questions range from simple know-how questions to more complex ones where knowledge utilization is tested by designing experimental approaches to invesitgate cellular processes.

Note:
The presentation of the assignment and attending the presentations of peers is mandatory, missing out on the presentations leads to having to redo the course or an alternative assignment.
Opgenomen in
Opleiding Jaar Periode Type
BSc Chemistry  ( Chemistry of life) 3 semester II a verplicht
BSc Courses for Exchange Students: Chemistry - Chemical Engineering - semester II a
BSc Natuurkunde  (Track: Biophysics and Medical Physics ) 3 semester II a verplicht