Skip to ContentSkip to Navigation
About usNews and EventsNews articles

Regulation of multidrug resistance in Lactococcus lactis

16 October 2009

PhD ceremony: ms. H. Agustiandari, 13.15 uur, Academiegebouw, Broerstraat 5, Groningen

Thesis: Regulation of multidrug resistance in Lactococcus lactis

Promotor(s): prof. A.J.M. Driessen

Faculty: Mathematics and Natural Sciences

 

Herfita Augustiandari studied the regulation of multidrug resistance in Lactococcus lactis. The multidrug resistance (MDR) phenomenon is the ability of cells to develop resistance against a wide range of structurally unrelated toxic molecules. MDR is caused by the (over) expression of transporters that expel these compounds from the cell. Multidrug resistance has lead to multiple complications in the treatment of for instance cancer or infectious diseases. The introduction and improper use of antibiotics have led to a selection of bacteria that by mutation(s) in their genome developed resistance mechanisms which allowed them to survive under this severe antibiotics pressure. In short the most important results of the research of Augustiandari are: She has elucidated the regulation mechanism of the major multidrug transporter LmrCD by the transcriptional regulator termed LmrR in Lactococcus lactis. She also showed that LmrR regulates its own expression but in a very different manner than to LmrCD. The crystal structures of apo LmrR and LmrR bound drugs (Hoechst 33342 and Daunomycin) were solved at 2.0Å and 2.2Å resolution, respectively where she found the presence of a symmetric big flat-shape central pore responsible for ligand(s) binding. This feature is unique and has not been seen before for the other well characterized bacterial transcriptional regulator. These new insights will be useful for the further development of the new antimicrobial compounds.

 

Last modified:15 September 2017 3.39 p.m.
printView this page in: Nederlands

More news

  • 17 October 2019

    iGEM 2019: Team Groningen develops a biological QR-code

    The team developed a protocol to share delicate information via a biological QR-code.

  • 14 October 2019

    Demonstrating that it can be done

    Moniek Tromp and Marleen Kamperman share a lot in common. Both are young professors of chemistry at the Zernike Institute for Advanced Materials (ZIAM).Both joined the UG in 2018. Both are engaged in developing new materials. Both enjoy promoting science...

  • 11 October 2019

    Down Under with Top Dutch

    After two years of hard work, the Groningen Top Dutch Solar Racing team has arrived in Australia. The team consists of students of the Hanze University of Applied Sciences, University of Groningen, and secondary vocational education (MBO) and is currently...