An international research group with, among others, Remko Winter and Marco Fraaije of the Groningen Biomolecular Sciences and Biotechnology Institute (GBB) of the University of Groningen, unravelled how enzymes ‘breath’. The outcomes were published in the online edition (16 june 2009) of PNAS, the Proceedings of the National Academy of Sciences of the United States of America.
Dioxygen (O2) and other gas molecules have a fundamental role in a variety of enzymatic reactions. However, it is only poorly understood which O2 uptake mechanism enzymes employ to promote efficient catalysis and how general this is. The research group investigated O2 diffusion pathways into monooxygenase and oxidase flavoenzymes, using an integrated computational and experimental approach. Enhanced-statistics molecular dynamics simulations reveal spontaneous protein-guided O2 diffusion from the bulk solvent to preorganized protein cavities. The predicted protein-guided diffusion paths and the importance of key cavity residues for oxygen diffusion were verified by combining site-directed mutagenesis, rapid kinetics experiments, and high-resolution X-ray structures. This study indicates that monooxygenase and oxidase flavoenzymes employ multiple funnel-shaped diffusion pathways to absorb O2 from the solvent and direct it to the reacting C4a atom of the flavin cofactor. The difference in O2 reactivity among dehydrogenases, monooxygenases, and oxidases ultimately resides in the fine modulation of the local environment embedding the reactive locus of the flavin.
The University of Groningen (UG) will soon have a new Bachelor’s degree programme on offer: Biomedical Engineering.
Parkinson’s disease causes brain cells that produce the neurotransmitter dopamine to die. But where does the disease start? Evidence suggests that a failure of the brain’s immune system may be the root cause of the disease.
The in-depth study “Future markets for renewable gases and hydrogen: What would be the optimal regulatory provisions?” by Professor José Luis Moraga, Professor Machiel Mulder and Peter Perey explores the economic outlook for renewable gases and hydrogen...