Skip to ContentSkip to Navigation
About usNews and EventsNews articles

Cell entry mechanisms of dengue virus

13 April 2009

PhD ceremony: H.M. van der Schaar, 14.45 uur, Academiegebouw, Broerstraat 5, Groningen

Thesis: Cell entry mechanisms of dengue virus

Promotor(s): prof. J.C. Wilschut

Faculty: Medical Sciences


Dengue virus (DENV) currently causes the most common mosquito-borne viral infection worldwide. According to the estimates of the World Health Organization, 50 to 100 million infections occur annually leading to 500,000 hospitalizations and 20,000 deaths. Despite this high impact, there are neither vaccines nor antiviral drugs available to prevent or treat DENV infections. DENV can cause disease in humans ranging from dengue fever, an illness with flu-like symptoms, to the more severe dengue hemorrhagic fever, characterized by potentially life-threatening bleeding manifestions. Antibodies are believed to play an important role in controlling disease severity by directly influencing the infectious properties of the virus. The studies presented in this thesis elucidate the mechanisms that DENV uses to enter and infect the host cell. First, we have followed individual fluorescently labeled virus particles in real-time as they enter a cell, in which different compartments are illuminated by fluorescent marker proteins. Thus, we have visualized the cell entry pathway of DENV and identified the compartment in which the virus undergoes membrane fusion to deliver its genome into the cell and initiate infection. Additionally, we have investigated the infectious properties of a subpopulation of DENV particles, called immature particles, that are considered to be non-infectious. Remarkably, with the help of antibodies, immature DENV particles are able to productively infect cells and therefore might participate in the pathogenesis of severe disease. Collectively, our findings have enriched our knowledge on the critical determinants in DENV infection and may contribute to the development of antiviral drugs and safe dengue vaccines.



Last modified:15 September 2017 3.39 p.m.
printView this page in: Nederlands

More news

  • 16 October 2019

    Nitrogen emissions pose risk to kidneys

    Elevated levels of nitrogen pose a risk to kidneys. This link has been revealed by a research project involving more than 143,000 inhabitants of the northern Netherlands, carried out by a number of UMCG nephrologists and researchers. Today they publish...

  • 15 October 2019

    Meiden die ‘aan de pil’ zijn hebben vaker depressieve gevoelens

    Zestienjarige meisjes die de anticonceptiepil gebruiken hebben vaker depressieve klachten dan leeftijdsgenoten die niet aan de pil zijn. Dat blijkt uit een studie van onderzoekers van het UMCG, LUMC en Brigham and Women’s Hospital. Ze publiceren hun...

  • 11 October 2019

    Down Under with Top Dutch (update)

    After two years of hard work, the Groningen Top Dutch Solar Racing team has arrived in Australia. The team consists of students of the Hanze University of Applied Sciences, University of Groningen, and secondary vocational education (MBO) and is currently...