Skip to ContentSkip to Navigation
About usNews and EventsNews articles

Water-in-oil emulsions as adjuvant inactivated vaccines

18 February 2009

PhD ceremony: T. Jansen, 14.45 uur, Academiegebouw, Broerstraat 5, Groningen

Thesis: Water-in-oil emulsions as adjuvant inactivated vaccines

Promotor(s): prof. D. Hoekstra, prof. V.E.J.C. Schrijns

Faculty: Medical Sciences

 

Water-in-oil emulsions are well-known immunopotentiators for inactivated vaccines. However, their structural requirements, instrumental in producing the highest antibody titre remain poorly understood.
In this thesis several physical characteristics of water-in-oil emulsions were studied in vaccine formulations containing inactivated Infectious Bronchitis virus, Newcastle Disease virus and Infectious Bursal Disease virus. These vaccines were evaluated in immunized chickens for magnitude and duration of in-vivo antiviral antibody formation.
High Mineral oil content proved most effective in causing an antibody response, compared to biodegradable Miglyol 840.
Direct comparison of three types of emulsions, i.e., water-in-oil, oil-in-water and water-in-oil-in-water, while maintaining an identical content of components for each vehicle showed that water-in-oil type emulsions induced the best immune response.
Assessing the inherent release capacity of emulsions in-vitro remarkably water-in-oil emulsions released almost no antigen during twelve weeks. The importance of controlled release of antigens was investigated by injecting chickens repeatedly with small doses of inactivated antigen during 48 days. We observed that this repeated administration proved less effective when compared to a single dose vaccination of the cumulative amount of antigen formulated in a water-in-oil emulsion. This may indicate presence of immunostimulatory activity of the oil phase besides prolonged antigen residence time.
Decreasing dosage volume of vaccines based on water-in-oil emulsions appeared to minimize local reaction scores. Our results demonstrate that the efficacy of antibody response was not significantly affected, in case the amount of antigen per dose was maintained constant.

 

Last modified:15 September 2017 3.39 p.m.
printView this page in: Nederlands

More news

  • 11 October 2019

    Down Under with Top Dutch

    After two years of hard work, the Groningen Top Dutch Solar Racing team has arrived in Australia. The team consists of students of the Hanze University of Applied Sciences, University of Groningen, and secondary vocational education (MBO) and is currently...

  • 08 October 2019

    Making a splash in whale research

    He disliked genetics as an undergraduate and never really wanted to work with whales. Yet, Per Palsbøll became a worldwide expert in the genetics of marine mammals, heading a research programme spanning the entire globe. Introducing the concept of...

  • 03 October 2019

    New criteria for bank loans and stock exchanges listings could protect depleted marine resources from seafood sector

    Two reforms in the finance sector have the potential to accelerate action towards a sustainable seafood industry, according to new research published in the journal Science Advances. Compiling data on 160 publicly listed seafood companies and 3000...