Dijkhuizen, Prof. Lubbert
Lubbert Dijkhuizen (1951) is professor of Microbiology. He initiated many research projects into life processes in micro-organisms and relevant industrial and other applications of this fundamental knowledge. The main focus in this research is on the ability to use micro-organisms and enzymes in biotechnological processes, in particular in the conversion of carbohydrates. Dijkhuizen's research also focuses on tuberculosis.
Dijkhuizen is one of the initiators and joint founders of the Carbohydrate Competence Center (CCC), a public-private cooperation among 19 businesses and 6 knowledge institutions. Research is being conducted in 24 projects (total value € 28 million) on carbohydrates that play an important role in Nutrition and Health. The Dagblad van het Noorden has written an overview article about the CCC's research and its implications for healthy nutrition, for example.
Dijkhuizen has thus far supervised over 55 PhD students and written over 300 international scientific publications. His research is internationally renowned and is so fruitful that he is always able to acquire the necessary funds. He has a good eye for the possible products and processes that his research may yield. This has resulted, for example, in his being mentioned as the inventor or joint inventor in 25 patents and about 75% of his research group being financed by external funds, mainly by industrial parties. In late 2010, Dijkhuizen and his colleague Dijkstra caused an international stir with an article in PNAS about a possible solution to prevent caries. In 2012 Dijkhuizen was nominated as one of the three most enterprising researchers of The Netherlands. the same year, Dijkhuizen's CCC announced a breakthrough in carbohydrate research, as a result of which, among other things, French Fries can be made healthier.
Previously in the news
- Research into carbohydrates shows promising results for healthy food and BioBased Economy ..
- Frisdrankfles uit suikerbiet (in Dutch)
- Breakthrough in research healthy French Fries (in Dutch)
- Vaccine for Rhodococcus equi infection in foals
- Breakthrough in dental plaque research
- Glucansucrase designated as Molecule of the Month by the Protein Data Bank (PDB)
- Groningen launches research centre for synthetic biology
Publications
2020
Hartman,
A. M., Jumde, V. R., Elgaher, W. A. M., Te Poele, E.
M., Dijkhuizen, L., & Hirsch, A. K.
H. (2021). Potential Dental Biofilm Inhibitors:
Dynamic Combinatorial Chemistry Affords Sugar-Based Molecules that
Target Bacterial Glucosyltransferase.
ChemMedChem, 16(1), 113-123.
[cmdc.202000222]. https://doi.org/10.1002/cmdc.202000222
Te
Poele, E., Corwin, S., Hamaker, B. R., Lamothe, L.,
Vafeiadi, C., & Dijkhuizen, L. (2020).
Development of Slowly Digestible Starch Derived
α-Glucans with 4,6-α-Glucanotransferase and Branching
Sucrase Enzymes. Journal of Agricultural and Food
Chemistry, 68(24), 6664-6671.
[acs.jafc.0c01465]. https://doi.org/10.1021/acs.jafc.0c01465
Knight,
J., Branneby, C. K., Dijkhuizen, L., Petrusma, M.,
& Fernández de las Heras, L. (2020). Genetically-modified bacteria and uses
thereof. (Patent No. WO2020030799).
van
Leeuwen, S. S., Te Poele, E. M., Chatziioannou, A. C.,
Benjamins, E., Haandrikman, A., & Dijkhuizen, L.
(2020). Goat Milk Oligosaccharides: Their Diversity,
Quantity, and Functional Properties in Comparison to Human Milk
Oligosaccharides. Journal of Agricultural and Food
Chemistry, 68(47), 13469-13485. https://doi.org/10.1021/acs.jafc.0c03766
Valk-Weeber,
R. L., Deelman-Driessen, C., Dijkhuizen,
L., Eshuis-de Ruiter, T., & van Leeuwen, S. (2020).
In depth analysis of the contribution of specific
glycoproteins to the overall bovine whey N-linked
glycoprofile. Journal of Agricultural and Food
Chemistry, 68(24), 6544-6553.
[acs.jafc.0c00959]. https://doi.org/10.1021/acs.jafc.0c00959
Figueroa-Lozano,
S., Valk-Weeber, R. L., Akkerman, R.,
Abdulahad, W., van Leeuwen, S. S., Dijkhuizen,
L., & de Vos, P. (2020). Inhibitory
Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like
Receptor 8; Comparing Efficacy With Chloroquine.
Frontiers in Immunology, 11, [790].
https://doi.org/10.3389/fimmu.2020.00790
Valk-Weeber,
R. L., Eshuis-de Ruiter, T., Dijkhuizen, L., & van
Leeuwen, S. S. (2020). Quantitative analysis of bovine whey
glycoproteins using the overall N-linked whey glycoprofile.
International Dairy Journal, 110,
[104814]. https://doi.org/10.1016/j.idairyj.2020.104814
Kittibunchakul,
S., van Leeuwen, S. S., Dijkhuizen, L., Haltrich, D.,
& Thu-Ha Nguyen (2020). Structural Comparison of
Different Galacto-oligosaccharide Mixtures Formed by
beta-Galactosidases from Lactic Acid Bacteria and
Bifidobacteria. Journal of Agricultural and Food
Chemistry, 68(15), 4437-4446. https://doi.org/10.1021/acs.jafc.9b08156
Li,
X., Wang, X., Meng, X., Dijkhuizen, L., & Liu, W.
(2020). Structures, physico-chemical properties, production
and (potential) applications of sucrose-derived α-d-glucans
synthesized by glucansucrases. Carbohydrate
Polymers, 249, [116818]. https://doi.org/10.1016/j.carbpol.2020.116818
Figueroa-Lozano,
S., Ren, C., Yin, H., Pham, H.,
van Leeuwen, S., Dijkhuizen, L., & de Vos,
P. (2020). The impact of oligosaccharide content,
glycosidic linkages and lactose content of galacto-oligosaccharides
(GOS) on the expression of mucus-related genes in goblet
cells. Food & Function,
11(4), 3506-3515. https://doi.org/10.1039/d0fo00064g
2019
Valk-Weeber,
R. L., Eshuis-de Ruiter, T., Dijkhuizen, L., & van
Leeuwen, S. S. (2020). Dynamic temporal variations in bovine
lactoferrin glycan structures. Journal of
Agricultural and Food Chemistry, 68(2),
549-560. [acs.jafc.9b06762]. https://doi.org/10.1021/acs.jafc.9b06762
Valk-Weeber,
R. L., Dijkhuizen, L., & van Leeuwen, S. S.
(2019). Large-scale quantitative isolation of pure protein
N-linked glycans. Carbohydrate
Research, 479, 13-22. https://doi.org/10.1016/j.carres.2019.04.011
Böger,
M., Hekelaar, J., van Leeuwen, S. S.,
Dijkhuizen, L., & Lammerts van Bueren, A. (2019).
Structural and functional characterization of a family GH53
β-1,4-galactanase from Bacteroides thetaiotaomicron that
facilitates degradation of prebiotic
galactooligosaccharides. Journal of Structural
Biology, 205(1), 1-10. [j.jsb.2018.12.002]. https://doi.org/10.1016/j.jsb.2018.12.002
Boger,
M., van Leeuwen, S. S., van Bueren, A. L., & Dijkhuizen,
L. (2019). Structural Identity of
Galactooligosaccharide Molecules Selectively Utilized by Single
Cultures of Probiotic Bacterial Strains. Journal
of Agricultural and Food Chemistry, 67(50),
13969-13977. https://doi.org/10.1021/acs.jafc.9b05968
Pham,
H., Ten Kate, G. A., Dijkhuizen, L., & van
Leeuwen, S. S. (2019). Synthesis and characterization of
sialylated lactose- and lactulose-derived oligosaccharides by
Trypanosoma cruzi trans-sialidase. Journal of
Agricultural and Food Chemistry, 67(12),
3469–3479. https://doi.org/10.1021/acs.jafc.8b06974
Devlamynck,
T., Te Poele, E. M., Quataert, K., Gerwig, G.
J., Van de Walle, D., Dewettinck, K., Kamerling, J. P.,
Soetaert, W., & Dijkhuizen, L. (2019).
Trans-α-glucosylation of stevioside by the mutant
glucansucrase enzyme Gtf180-ΔN-Q1140E improves its taste
profile. Food Chemistry, 272,
653-662. https://doi.org/10.1016/j.foodchem.2018.08.025
2018
Pham,
H. T. T., Boger, M. C. L., Dijkhuizen, L., & van
Leeuwen, S. S. (2019). Stimulatory effects of novel
glucosylated lactose derivatives GL34 on growth of selected gut
bacteria. Applied Microbiology and
Biotechnology, 103(2), 707-718. https://doi.org/10.1007/s00253-018-9473-8
Meng,
X., Gangoiti, J., Wang, X., Grijpstra, P., van
Leeuwen, S. S., Pijning, T., & Dijkhuizen,
L. (2018). Biochemical characterization of a GH70
protein from Lactobacillus kunkeei DSM 12361 with two catalytic
domains involving branching sucrase activity.
Applied Microbiology and Biotechnology,
102(18), 7935-7950. https://doi.org/10.1007/s00253-018-9236-6
Meng,
X., Gangoiti, J., de Kok, N., van Leeuwen, S.
S., Pijning, T., & Dijkhuizen, L.
(2018). Biochemical characterization of two GH70 family
4,6-α-glucanotransferases with distinct product specificity
from Lactobacillus aviarius subsp. aviarius DSM 20655.
Food Chemistry, 253, 236-246.
[j.foodchem.2018.01.154]. https://doi.org/10.1016/j.foodchem.2018.01.154
Gangoiti
Muñecas, J., van Leeuwen, S. S., Pijning,
T., Dijkhuizen, L., Vafeiadi, C., & Duboux,
S. (2018). Branched alpha glucans.
(Patent No. WO2018167032). https://nl.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2018167032A1&KC=A1&FT=D&ND=3&date=20180920&DB=&locale=nl_NL
Boger,
M. C. L., van Bueren, A. L., & Dijkhuizen, L.
(2018). Cross-Feeding among Probiotic Bacterial Strains on
Prebiotic Inulin Involves the Extracellular exo-Inulinase of
Lactobacillus paracasei Strain W20. Applied and
environmental microbiology, 84(21),
[e01539-18]. https://doi.org/10.1128/AEM.01539-18
Te
Poele, E. M., Devlamynck, T., Jäger, M., Gerwig,
G. J., Van de Walle, D., Dewettinck, K., Hirsch, A. K.
H., Kamerling, J. P., Soetaert, W., & Dijkhuizen,
L. (2018). Glucansucrase (mutant) enzymes from
Lactobacillus reuteri 180 efficiently transglucosylate Stevia
component rebaudioside A, resulting in a superior taste.
Scientific Reports, 8(1), [1516]. https://doi.org/10.1038/s41598-018-19622-5
Pham,
H., Pijning, T., Dijkhuizen, L., &
van Leeuwen, S. S. (2018). Mutational Analysis of the Role of
the Glucansucrase Gtf180-Delta N Active Site Residues in Product
and Linkage Specificity with Lactose as Acceptor Substrate.
Journal of Agricultural and Food Chemistry,
66(47), 12544-12554. [acs.jafc.8b04486]. https://doi.org/10.1021/acs.jafc.8b04486
Lammerts
van Bueren-Brandt, A., & Dijkhuizen, L.
(2018). Prebiotic branched
galacto-oligosaccharides (gos). (Patent No.
WO2018048305). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2018048305A1&KC=A1&FT=D&ND=3&date=20180315&DB=&locale=en_EP
van
Leeuwen, S. S., Stoutjesdijk, E., ten Kate, G. A., Schaafsma,
A., Dijck-Brouwer, J., Muskiet, F. A. J., &
Dijkhuizen, L. (2018). Regional variations in human
milk oligosaccharides in Vietnam suggest FucTx activity besides
FucT2 and FucT3. Scientific Reports,
8(1), [16790]. https://doi.org/10.1038/s41598-018-34882-x
Pham,
H. T., Dijkhuizen, L., & van Leeuwen, S. S.
(2018). Structural characterization of glucosylated GOS
derivatives synthesized by the Lactobacillus reuteri GtfA and
Gtf180 glucansucrase enzymes. Carbohydrate
Research, 470, 57-63. https://doi.org/10.1016/j.carres.2018.10.003
Yin,
H., Dijkhuizen, L., & van Leeuwen, S. (2018).
Synthesis of galacto-oligosaccharides derived from lactulose
by wild-type and mutant β-galactosidase enzymes from Bacillus
circulans ATCC 31382. Carbohydrate
Research, 465, 58-65. [j.carres.2018.06.009].
https://doi.org/10.1016/j.carres.2018.06.009
Gangoiti,
J., Corwin, S. F., Lamothe, L. M., Vafiadi, C., Hamaker, B.
R., & Dijkhuizen, L. (2018). Synthesis of
novel α-glucans with potential health benefits through
controlled glucose release in the human gastrointestinal
tract. Critical Reviews in Food Science and
Nutrition, 60(1), 123-146. https://doi.org/10.1080/10408398.2018.1516621
2017
Gangoiti,
J., Pijning, T., & Dijkhuizen, L.
(2018). Biotechnological potential of novel glycoside
hydrolase family 70 enzymes synthesizing α-glucans from starch
and sucrose. Biotechnology Advances,
36, 196-207. https://doi.org/10.1016/j.biotechadv.2017.11.001
Figueroa-Lozano,
S., Valk-Weeber, R. L., van Leeuwen, S. S.,
Dijkhuizen, L., & de Vos, P. (2018).
Dietary N-glycans from Bovine Lactoferrin and TLR
Modulation. Molecular Nutrition & Food
Research, 62(2), [1700389]. https://doi.org/10.1002/mnfr.201700389
Sarian,
F. D., Janeček, Š., Pijning, T., Ihsanawati,
Nurachman, Z., Radjasa, O. K., Dijkhuizen, L.,
Natalia, D., & van der Maarel, M. J. E. C. (2017).
A new group of glycoside hydrolase family 13 α-amylases
with an aberrant catalytic triad. Scientific
Reports, 7, [44230]. https://doi.org/10.1038/srep44230
Dijkhuizen,
L., Gangoiti Muñecas, J., van Leeuwen, S. S., Vafeiadi,
C., & Duboux, S. (2017). Alpha
glucans. (Patent No. WO2017207663). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2017207663A1&KC=A1&FT=D&ND=3&date=20171207&DB=&locale=en_EP
Yin,
H., Pijning, T., Meng, X., Dijkhuizen,
L., & van Leeuwen, S. S. (2017). Biochemical
characterization of the functional roles of residues in the active
site of the β-galactosidase from Bacillus circulans ATCC
31382. Biochemistry, 56(24),
3109-3118. https://doi.org/10.1021/acs.biochem.7b00207
Gangoiti
Muñecas, J., van Leeuwen, S. S., Dijkhuizen, L.,
Vafeiadi, C., & Lamothe, L. (2017). Branched alpha glucans. (Patent No.
WO2017046040). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2017046040A1&KC=A1&FT=D&ND=3&date=20170323&DB=&locale=en_EP
te
Poele, E. M., Valk, V., Devlamynck, T., van Leeuwen, S.
S., & Dijkhuizen, L. (2017). Catechol
glucosides act as donor/acceptor substrates of glucansucrase
enzymes of Lactobacillus reuteri. Applied
Microbiology and Biotechnology, 101(11),
4495-4505. https://doi.org/10.1007/s00253-017-8190-z
Meng,
X., Pijning, T., Tietema, M., Dobruchowska, J. M.,
Yin, H., Gerwig, G. J., Kralj, S., &
Dijkhuizen, L. (2017). Characterization of the
glucansucrase GTF180 W1065 mutant enzymes producing polysaccharides
and oligosaccharides with altered linkage composition.
Food Chemistry, 217, 81-90. https://doi.org/10.1016/j.foodchem.2016.08.087
Gangoiti,
J., Lamothe, L., van Leeuwen, S. S., Vafiadi, C., &
Dijkhuizen, L. (2017). Characterization of the
Paenibacillus beijingensis DSM 24997 GtfD and its glucan polymer
products representing a new glycoside hydrolase 70 subfamily of
4,6-α-glucanotransferase enzymes. PLoS
ONE, 12(4), [e0172622]. https://doi.org/10.1371/journal.pone.0172622
Gangoiti
Muñecas, J., Meng, X., Lamerts van Bueren,
A., & Dijkhuizen, L. (2017). Draft
Genome Sequence of Lactobacillus reuteri 121, a Source of
α-Glucan and β-Fructan Exopolysaccharides.
Genome Announcements, 5(10), 1-2.
[e01691-16]. https://doi.org/10.1128/genomeA.01691-16
Yin,
H., Pijning, T., Meng, X., Dijkhuizen,
L., & van Leeuwen, S. S. (2017). Engineering of
the Bacillus circulans β-galactosidase product
specificity. Biochemistry,
56(5), 704-711. https://doi.org/10.1021/acs.biochem.7b00032
Ceniceros,
A., Dijkhuizen, L., Petrusma, M., & Medema, M. H.
(2017). Genome-based exploration of the specialized metabolic
capacities of the genus Rhodococcus. BMC
Genomics, 18(1), 1-16. [593]. https://doi.org/10.1186/s12864-017-3966-1
Gangoiti,
J., van Leeuwen, S. S., Meng, X., Duboux, S., Vafiadi, C.,
Pijning, T., & Dijkhuizen, L. (2017).
Mining novel starch-converting Glycoside Hydrolase 70 enzymes
from the Nestlé Culture Collection genome database: The
Lactobacillus reuteri NCC 2613 GtfB. Scientific
Reports, 7(1), [9947]. https://doi.org/10.1038/s41598-017-07190-z
Frasch,
H-J., Leeuwen, S. S. V., & Dijkhuizen, L. (2017).
Molecular and biochemical characteristics of the inulosucrase
HugO from Streptomyces viridochromogenes DSM40736
(Tü494). Microbiology-Reading,
163(7), 1030-1041. https://doi.org/10.1099/mic.0.000493
Ceniceros,
A., Dijkhuizen, L., & Petrusma, M. (2017).
Molecular characterization of a Rhodococcus jostii RHA1
γ-butyrolactone(-like) signalling molecule and its main
biosynthesis gene gblA. Scientific
Reports, 7(1), 1-13. [17743]. https://doi.org/10.1038/s41598-017-17853-6
Lammerts
van Bueren, A., Mulder, M., Leeuwen, S. V., &
Dijkhuizen, L. (2017). Prebiotic
galactooligosaccharides activate mucin and pectic galactan
utilization pathways in the human gut symbiont Bacteroides
thetaiotaomicron. Scientific Reports,
7, 1-13. [40478]. https://doi.org/10.1038/srep40478
Yin,
H., Bultema, J. B., Dijkhuizen, L., & van Leeuwen,
S. S. (2017). Reaction kinetics and galactooligosaccharide
product profiles of the β-galactosidases from Bacillus
circulans, Kluyveromyces lactis and Aspergillus oryzae.
Food Chemistry, 225, 230-238.
[j.foodchem.2017.01.030]. https://doi.org/10.1016/j.foodchem.2017.01.030
Gerwig,
G. J., Te Poele, E. M., Dijkhuizen,
L., & Kamerling, J. P. (2017). Structural analysis
of rebaudioside A derivatives obtained by Lactobacillus reuteri 180
glucansucrase-catalyzed trans-α-glucosylation.
Carbohydrate Research, 440-441,
51-62. https://doi.org/10.1016/j.carres.2017.01.008
Pham,
H. T. T., Dijkhuizen, L., & van Leeuwen, S. S.
(2017). Structural characterization of glucosylated lactose
derivatives synthesized by the Lactobacillus reuteri GtfA and
Gtf180 glucansucrase enzymes. Carbohydrate
Research, 449, 59-64. https://doi.org/10.1016/j.carres.2017.07.002
Montersino,
S., te Poele, E., Orru, R., Westphal, A. H.,
Barendregt, A., Heck, A. J. R., van der Geize, R.,
Dijkhuizen, L., Mattevi, A., & van Berkel, W. J. H.
(2017). 3-Hydroxybenzoate 6-Hydroxylase from Rhodococcus
jostii RHA1 Contains a Phosphatidylinositol Cofactor.
Frontiers in Microbiology, 8, 1-11.
[1110]. https://doi.org/10.3389/fmicb.2017.01110
Gangoiti
Muñecas, J., van Leeuwen, S. S., Gerwig, G. J.,
Duboux, S., Vafiadi, C., Pijning, T., &
Dijkhuizen, L. (2017). 4,3-α-Glucanotransferase,
a novel reaction specificity in glycoside hydrolase family 70 and
clan GH-H. Scientific Reports,
7, 1-15. [39761]. https://doi.org/10.1038/srep39761
2016
Bai,
Y., Gangoiti , J., Dijkstra, B. W., Dijkhuizen,
L., & Pijning, T. (2017). Crystal
Structure of 4,6-α-Glucanotransferase Supports Diet-Driven
Evolution of GH70 Enzymes from α-Amylases in Oral
Bacteria. Structure, 25(2),
231-242. https://doi.org/10.1016/j.str.2016.11.023
Valk,
V., Lammerts van Bueren, A., van der Kaaij, R.
M., & Dijkhuizen, L. (2016).
Carbohydrate Binding Module 74 is a novel starch binding
domain associated with large and multi-domain α-amylase
enzymes. Febs Journal,
283(12), 2354-2368. [13745]. https://doi.org/10.1111/febs.13745
Valk,
V., van der Kaaij, R. M., & Dijkhuizen,
L. (2016). Characterization of the starch-acting
MaAmyB enzyme from Microbacterium aurum B8.A representing the novel
subfamily GH13_42 with an unusual, multi-domain
organization. Scientific Reports,
6, 1-12. [srep36100]. https://doi.org/10.1038/srep36100
van
Leeuwen, S. S., Kuipers, B. J. H., Dijkhuizen, L.,
& Kamerling, J. P. (2016). Comparative structural
characterization of 7 commercial galacto-oligosaccharide (GOS)
products. Carbohydrate Research,
425, 48-58. https://doi.org/10.1016/j.carres.2016.03.006
van
Leeuwen, S. S., Kuipers, B. J. H., Dijkhuizen, L.,
& Kamerling, J. P. (2016). Corrigendum to “1H NMR
analysis of the lactose/β-galactosidase-derived
galacto-oligosaccharide components of Vivinal® GOS up to
DP5” [Carbohydr. Res. 400 (2014) 59–73].
Carbohydrate Research, 419, 69-70.
https://doi.org/10.1016/j.carres.2015.12.004
Pijning,
T., Bai, Y., Gangoiti Muñecas, J., &
Dijkhuizen, L. (2016). Crystal Structure of a
4,6-α-Glucanotransferase Supports Diet-Driven Evolution of
GH70 Enzymes from α-Amylases in Oral Bacteria.
Poster session presented at ALAMY, Smolenice, Slovakia.
Figueroa,
S., Dijkhuizen, L., Valk, R., van Leeuwen, S., &
de Vos, P. (2016). Dietary N- and O-glycans from cow milk and
TLR modulation. European Journal of
Immunology, 46, 479-479.
Devlamynck,
T., Te Poele, E. M., Meng, X., van Leeuwen, S.
S., & Dijkhuizen, L. (2016). Glucansucrase
Gtf180-Delta N of Lactobacillus reuteri 180: enzyme and reaction
engineering for improved glycosylation of non-carbohydrate
molecules. Applied Microbiology and
Biotechnology, 100(17), 7529-7539. https://doi.org/10.1007/s00253-016-7476-x
Te
Poele, E. M., Grijpstra, P., van Leeuwen, S.
S., & Dijkhuizen, L. (2016). Glucosylation
of catechol with the GTFA glucansucrase enzyme from Lactobacillus
reuteri and sucrose as donor substrate.
BIOCONJUGATE CHEMISTRY, 27(4),
937-946. https://doi.org/10.1021/acs.bioconjchem.6b00018
Bai,
Y., Böger, M., van der Kaaij, R. M.,
Woortman, A. J. J., Pijning, T., van Leeuwen,
S. S., Lammerts van Bueren, A., &
Dijkhuizen, L. (2016). Lactobacillus reuteri strains
convert starch and maltodextrins into homo-exopolysaccharides using
an extracellular and cell-associated
4,6-α-glucanotransferase. Journal of
Agricultural and Food Chemistry, 64(14),
2941-2952. https://doi.org/10.1021/acs.jafc.6b00714
te
Poele, E. M., Dijkhuizen, L., Gerwig, G.
J., & Kamerling, J. P. (2016). Methods for the enzymatic modification of steviol
glycosides, modified steviol glycosides obtainable thereby, and the
use thereof as sweeteners. (Patent No.
WO2016144175). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2016144175A1&KC=A1&FT=D&ND=3&date=20160915&DB=EPODOC&locale=en_EP
Dobruchowska,
J. M., Jonsson, J. O., Fridjonsson, O. H., Aevarsson, A.,
Kristjansson, J. K., Altenbuchner, J., Watzlawick, H.,
Gerwig, G. J., Dijkhuizen, L., Kamerling, J.
P., & Hreggvidsson, G. O. (2016). Modification of linear
(β1→3)-linked gluco-oligosaccharides with a novel
recombinant β-glucosyltransferase (trans-β-glucosidase)
enzyme from Bradyrhizobium diazoefficiens.
Glycobiology, 26(11), 1157-1170. https://doi.org/10.1093/glycob/cww074
Gerwig,
G. J., Te Poele, E. M., Dijkhuizen,
L., & Kamerling, J. P. (2016). Stevia Glycosides:
Chemical and Enzymatic Modifications of Their Carbohydrate Moieties
to Improve the Sweet-Tasting Quality. Advances in
carbohydrate chemistry and biochemistry, 73,
1-72. https://doi.org/10.1016/bs.accb.2016.05.001
Bai,
Y., Dobruchowska, J. M., van der Kaaij, R. M.,
Gerwig, G. J., & Dijkhuizen, L. (2016).
Structural basis for the roles of starch and sucrose in
homo-exopolysaccharide formation by Lactobacillus reuteri
35-5. Carbohydrate Polymers,
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