Dijkhuizen, Prof. Lubbert
Lubbert Dijkhuizen (1951) is professor of Microbiology. He initiated many research projects into life processes in micro-organisms and relevant industrial and other applications of this fundamental knowledge. The main focus in this research is on the ability to use micro-organisms and enzymes in biotechnological processes, in particular in the conversion of carbohydrates. Dijkhuizen's research also focuses on tuberculosis.
Dijkhuizen is one of the initiators and joint founders of the Carbohydrate Competence Center (CCC), a public-private cooperation among 19 businesses and 6 knowledge institutions. Research is being conducted in 24 projects (total value € 28 million) on carbohydrates that play an important role in Nutrition and Health. The Dagblad van het Noorden has written an overview article about the CCC's research and its implications for healthy nutrition, for example.
Dijkhuizen has thus far supervised over 55 PhD students and written over 300 international scientific publications. His research is internationally renowned and is so fruitful that he is always able to acquire the necessary funds. He has a good eye for the possible products and processes that his research may yield. This has resulted, for example, in his being mentioned as the inventor or joint inventor in 25 patents and about 75% of his research group being financed by external funds, mainly by industrial parties. In late 2010, Dijkhuizen and his colleague Dijkstra caused an international stir with an article in PNAS about a possible solution to prevent caries. In 2012 Dijkhuizen was nominated as one of the three most enterprising researchers of The Netherlands. the same year, Dijkhuizen's CCC announced a breakthrough in carbohydrate research, as a result of which, among other things, French Fries can be made healthier.
Previously in the news
- Research into carbohydrates shows promising results for healthy food and BioBased Economy ..
- Frisdrankfles uit suikerbiet (in Dutch)
- Breakthrough in research healthy French Fries (in Dutch)
- Vaccine for Rhodococcus equi infection in foals
- Breakthrough in dental plaque research
- Glucansucrase designated as Molecule of the Month by the Protein Data Bank (PDB)
- Groningen launches research centre for synthetic biology
Publications
2021
Jiang,
Y., Li, X., Pijning, T., Bai, Y., &
Dijkhuizen, L. (2022). Mutations in Amino Acid
Residues of Limosilactobacillus reuteri 121 GtfB
4,6-α-Glucanotransferase that Affect Reaction and Product
Specificity. Journal of Agricultural and Food
Chemistry, 70(6), 1952-1961. [1c07618]. https://doi.org/10.1021/acs.jafc.1c07618
Li,
X., Meng, X., de Leeuw, T. C., Te Poele, E. M.,
Pijning, T., Dijkhuizen, L., & Liu, W.
(2021). Enzymatic glucosylation of polyphenols using
glucansucrases and branching sucrases of glycoside hydrolase family
70. Critical Reviews in Food Science and
Nutrition, 1-21. [2016598]. https://doi.org/10.1080/10408398.2021.2016598
Chatziioannou,
A. C., Benjamins, E., Pellis, L., Haandrikman, A.,
Dijkhuizen, L., & van Leeuwen, S. S.
(2021). Extraction and Quantitative Analysis of Goat Milk
Oligosaccharides: Composition, Variation, Associations, and 2 '-FL
Variability. Journal of Agricultural and Food
Chemistry, 69(28), 7851-7862. https://doi.org/10.1021/acs.jafc.1c00499
Pijning,
T., Gangoiti, J., Te Poele, E. M.,
Börner, T., & Dijkhuizen, L. (2021).
Insights into Broad-Specificity Starch Modification from the
Crystal Structure of Limosilactobacillus Reuteri NCC 2613
4,6-α-Glucanotransferase GtfB. Journal of
Agricultural and Food Chemistry, 69(44),
13235-13245. [05657]. https://doi.org/10.1021/acs.jafc.1c05657
Valk-Weeber,
R. L., Nichols, K., Dijkhuizen, L., Bijl, E.,
& van Leeuwen, S. S. (2021). Variations in
N-linked glycosylation of glycosylation-dependent cell adhesion
molecule 1 (GlyCAM-1) whey protein: Intercow differences and
dietary effects. Journal of dairy
science, 104(4), 5056-5068. https://doi.org/10.3168/jds.2020-19297
Figueroa
Lozano, S., Akkerman, R., Beukema,
M., van Leeuwen, S., Dijkhuizen,
L., & de Vos, P. (2021). 2
'-Fucosyllactose impacts the expression of mucus-related genes in
goblet cells and maintains barrier function of gut epithelial
cells. Journal of Functional Foods,
85, 1-8. [104630]. https://doi.org/10.1016/j.jff.2021.104630
2020
Hartman,
A. M., Jumde, V. R., Elgaher, W. A. M., Te Poele, E.
M., Dijkhuizen, L., & Hirsch, A. K.
H. (2021). Potential Dental Biofilm Inhibitors:
Dynamic Combinatorial Chemistry Affords Sugar-Based Molecules that
Target Bacterial Glucosyltransferase.
ChemMedChem, 16(1), 113-123.
[cmdc.202000222]. https://doi.org/10.1002/cmdc.202000222
Te
Poele, E., Corwin, S., Hamaker, B. R., Lamothe, L.,
Vafeiadi, C., & Dijkhuizen, L. (2020).
Development of Slowly Digestible Starch Derived
α-Glucans with 4,6-α-Glucanotransferase and Branching
Sucrase Enzymes. Journal of Agricultural and Food
Chemistry, 68(24), 6664-6671.
[acs.jafc.0c01465]. https://doi.org/10.1021/acs.jafc.0c01465
Knight,
J., Branneby, C. K., Dijkhuizen, L., Petrusma, M.,
& Fernández de las Heras, L. (2020). Genetically-modified bacteria and uses
thereof. (Patent No. WO2020030799).
van
Leeuwen, S. S., Te Poele, E. M., Chatziioannou,
A. C., Benjamins, E., Haandrikman, A., & Dijkhuizen,
L. (2020). Goat Milk Oligosaccharides: Their
Diversity, Quantity, and Functional Properties in Comparison to
Human Milk Oligosaccharides. Journal of
Agricultural and Food Chemistry, 68(47),
13469-13485. https://doi.org/10.1021/acs.jafc.0c03766
Valk-Weeber,
R. L., Deelman-Driessen, C., Dijkhuizen,
L., Eshuis-de Ruiter, T., & van Leeuwen, S.
(2020). In depth analysis of the contribution of specific
glycoproteins to the overall bovine whey N-linked
glycoprofile. Journal of Agricultural and Food
Chemistry, 68(24), 6544-6553.
[acs.jafc.0c00959]. https://doi.org/10.1021/acs.jafc.0c00959
Figueroa-Lozano,
S., Valk-Weeber, R. L., Akkerman, R.,
Abdulahad, W., van Leeuwen, S. S.,
Dijkhuizen, L., & de Vos, P. (2020).
Inhibitory Effects of Dietary N-Glycans From Bovine
Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With
Chloroquine. Frontiers in Immunology,
11, [790]. https://doi.org/10.3389/fimmu.2020.00790
Valk-Weeber,
R. L., Eshuis-de Ruiter, T., Dijkhuizen, L.,
& van Leeuwen, S. S. (2020). Quantitative analysis
of bovine whey glycoproteins using the overall N-linked whey
glycoprofile. International Dairy
Journal, 110, [104814]. https://doi.org/10.1016/j.idairyj.2020.104814
Kittibunchakul,
S., van Leeuwen, S. S., Dijkhuizen, L.,
Haltrich, D., & Thu-Ha Nguyen (2020). Structural
Comparison of Different Galacto-oligosaccharide Mixtures Formed by
beta-Galactosidases from Lactic Acid Bacteria and
Bifidobacteria. Journal of Agricultural and Food
Chemistry, 68(15), 4437-4446. https://doi.org/10.1021/acs.jafc.9b08156
Li,
X., Wang, X., Meng, X., Dijkhuizen, L., & Liu, W.
(2020). Structures, physico-chemical properties, production
and (potential) applications of sucrose-derived α-d-glucans
synthesized by glucansucrases. Carbohydrate
Polymers, 249, [116818]. https://doi.org/10.1016/j.carbpol.2020.116818
Figueroa-Lozano,
S., Ren, C., Yin, H., Pham,
H., van Leeuwen, S., Dijkhuizen,
L., & de Vos, P. (2020). The impact
of oligosaccharide content, glycosidic linkages and lactose content
of galacto-oligosaccharides (GOS) on the expression of
mucus-related genes in goblet cells. Food &
Function, 11(4), 3506-3515. https://doi.org/10.1039/d0fo00064g
2019
Valk-Weeber,
R. L., Eshuis-de Ruiter, T., Dijkhuizen, L.,
& van Leeuwen, S. S. (2020). Dynamic temporal
variations in bovine lactoferrin glycan structures.
Journal of Agricultural and Food Chemistry,
68(2), 549-560. [acs.jafc.9b06762]. https://doi.org/10.1021/acs.jafc.9b06762
Valk-Weeber,
R. L., Dijkhuizen, L., & van Leeuwen, S.
S. (2019). Large-scale quantitative isolation of pure
protein N-linked glycans. Carbohydrate
Research, 479, 13-22. https://doi.org/10.1016/j.carres.2019.04.011
Böger,
M., Hekelaar, J., van Leeuwen, S.
S., Dijkhuizen, L., & Lammerts van Bueren,
A. (2019). Structural and functional characterization of a
family GH53 β-1,4-galactanase from Bacteroides
thetaiotaomicron that facilitates degradation of prebiotic
galactooligosaccharides. Journal of Structural
Biology, 205(1), 1-10. [j.jsb.2018.12.002]. https://doi.org/10.1016/j.jsb.2018.12.002
Boger,
M., van Leeuwen, S. S., van Bueren, A. L., &
Dijkhuizen, L. (2019). Structural Identity of
Galactooligosaccharide Molecules Selectively Utilized by Single
Cultures of Probiotic Bacterial Strains. Journal
of Agricultural and Food Chemistry, 67(50),
13969-13977. https://doi.org/10.1021/acs.jafc.9b05968
Pham,
H., Ten Kate, G. A., Dijkhuizen, L., & van
Leeuwen, S. S. (2019). Synthesis and characterization
of sialylated lactose- and lactulose-derived oligosaccharides by
Trypanosoma cruzi trans-sialidase. Journal of
Agricultural and Food Chemistry, 67(12),
3469–3479. https://doi.org/10.1021/acs.jafc.8b06974
Devlamynck,
T., Te Poele, E. M., Quataert, K., Gerwig, G.
J., Van de Walle, D., Dewettinck, K., Kamerling, J. P.,
Soetaert, W., & Dijkhuizen, L. (2019).
Trans-α-glucosylation of stevioside by the mutant
glucansucrase enzyme Gtf180-ΔN-Q1140E improves its taste
profile. Food Chemistry, 272,
653-662. https://doi.org/10.1016/j.foodchem.2018.08.025
2018
Gangoiti,
J., Corwin, S. F., Lamothe, L. M., Vafiadi, C., Hamaker, B.
R., & Dijkhuizen, L. (2020). Synthesis of
novel α-glucans with potential health benefits through
controlled glucose release in the human gastrointestinal
tract. Critical Reviews in Food Science and
Nutrition, 60(1), 123-146. https://doi.org/10.1080/10408398.2018.1516621
Pham,
H. T. T., Boger, M. C. L., Dijkhuizen, L., &
van Leeuwen, S. S. (2019). Stimulatory effects of
novel glucosylated lactose derivatives GL34 on growth of selected
gut bacteria. Applied Microbiology and
Biotechnology, 103(2), 707-718. https://doi.org/10.1007/s00253-018-9473-8
Meng,
X., Gangoiti, J., Wang, X., Grijpstra, P., van
Leeuwen, S. S., Pijning, T., &
Dijkhuizen, L. (2018). Biochemical characterization of
a GH70 protein from Lactobacillus kunkeei DSM 12361 with two
catalytic domains involving branching sucrase activity.
Applied Microbiology and Biotechnology,
102(18), 7935-7950. https://doi.org/10.1007/s00253-018-9236-6
Meng,
X., Gangoiti, J., de Kok, N., van
Leeuwen, S. S., Pijning, T., &
Dijkhuizen, L. (2018). Biochemical characterization of
two GH70 family 4,6-α-glucanotransferases with distinct
product specificity from Lactobacillus aviarius subsp. aviarius DSM
20655. Food Chemistry, 253,
236-246. [j.foodchem.2018.01.154]. https://doi.org/10.1016/j.foodchem.2018.01.154
Gangoiti
Muñecas, J., van Leeuwen, S. S.,
Pijning, T., Dijkhuizen, L., Vafeiadi, C.,
& Duboux, S. (2018). Branched alpha
glucans. (Patent No. WO2018167032). https://nl.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2018167032A1&KC=A1&FT=D&ND=3&date=20180920&DB=&locale=nl_NL
Boger,
M. C. L., van Bueren, A. L., & Dijkhuizen, L.
(2018). Cross-Feeding among Probiotic Bacterial Strains on
Prebiotic Inulin Involves the Extracellular exo-Inulinase of
Lactobacillus paracasei Strain W20. Applied and
environmental microbiology, 84(21),
[e01539-18]. https://doi.org/10.1128/AEM.01539-18
Te
Poele, E. M., Devlamynck, T., Jäger, M., Gerwig,
G. J., Van de Walle, D., Dewettinck, K., Hirsch, A. K.
H., Kamerling, J. P., Soetaert, W., & Dijkhuizen,
L. (2018). Glucansucrase (mutant) enzymes from
Lactobacillus reuteri 180 efficiently transglucosylate Stevia
component rebaudioside A, resulting in a superior taste.
Scientific Reports, 8(1), [1516]. https://doi.org/10.1038/s41598-018-19622-5
Pham,
H., Pijning, T., Dijkhuizen, L.,
& van Leeuwen, S. S. (2018). Mutational Analysis
of the Role of the Glucansucrase Gtf180-Delta N Active Site
Residues in Product and Linkage Specificity with Lactose as
Acceptor Substrate. Journal of Agricultural and
Food Chemistry, 66(47), 12544-12554.
[acs.jafc.8b04486]. https://doi.org/10.1021/acs.jafc.8b04486
Lammerts
van Bueren-Brandt, A., & Dijkhuizen, L.
(2018). Prebiotic branched
galacto-oligosaccharides (gos). (Patent No.
WO2018048305). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2018048305A1&KC=A1&FT=D&ND=3&date=20180315&DB=&locale=en_EP
van
Leeuwen, S. S., Stoutjesdijk, E., ten Kate, G. A.,
Schaafsma, A., Dijck-Brouwer, J., Muskiet, F. A.
J., & Dijkhuizen, L. (2018). Regional
variations in human milk oligosaccharides in Vietnam suggest FucTx
activity besides FucT2 and FucT3. Scientific
Reports, 8(1), [16790]. https://doi.org/10.1038/s41598-018-34882-x
Pham,
H. T., Dijkhuizen, L., & van Leeuwen, S.
S. (2018). Structural characterization of glucosylated
GOS derivatives synthesized by the Lactobacillus reuteri GtfA and
Gtf180 glucansucrase enzymes. Carbohydrate
Research, 470, 57-63. https://doi.org/10.1016/j.carres.2018.10.003
Yin,
H., Dijkhuizen, L., & van Leeuwen, S.
(2018). Synthesis of galacto-oligosaccharides derived from
lactulose by wild-type and mutant β-galactosidase enzymes from
Bacillus circulans ATCC 31382. Carbohydrate
Research, 465, 58-65. [j.carres.2018.06.009].
https://doi.org/10.1016/j.carres.2018.06.009
2017
Gangoiti,
J., Pijning, T., & Dijkhuizen,
L. (2018). Biotechnological potential of novel
glycoside hydrolase family 70 enzymes synthesizing α-glucans
from starch and sucrose. Biotechnology
Advances, 36, 196-207. https://doi.org/10.1016/j.biotechadv.2017.11.001
Figueroa-Lozano,
S., Valk-Weeber, R. L., van Leeuwen, S.
S., Dijkhuizen, L., & de Vos,
P. (2018). Dietary N-glycans from Bovine Lactoferrin
and TLR Modulation. Molecular Nutrition & Food
Research, 62(2), [1700389]. https://doi.org/10.1002/mnfr.201700389
Sarian,
F. D., Janeček, Š., Pijning, T., Ihsanawati,
Nurachman, Z., Radjasa, O. K., Dijkhuizen, L.,
Natalia, D., & van der Maarel, M. J. E. C. (2017).
A new group of glycoside hydrolase family 13 α-amylases
with an aberrant catalytic triad. Scientific
Reports, 7, [44230]. https://doi.org/10.1038/srep44230
Dijkhuizen,
L., Gangoiti Muñecas, J., van
Leeuwen, S. S., Vafeiadi, C., & Duboux, S. (2017). Alpha glucans. (Patent No.
WO2017207663). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2017207663A1&KC=A1&FT=D&ND=3&date=20171207&DB=&locale=en_EP
Yin,
H., Pijning, T., Meng, X., Dijkhuizen,
L., & van Leeuwen, S. S. (2017).
Biochemical characterization of the functional roles of
residues in the active site of the β-galactosidase from
Bacillus circulans ATCC 31382.
Biochemistry, 56(24), 3109-3118. https://doi.org/10.1021/acs.biochem.7b00207
Gangoiti
Muñecas, J., van Leeuwen, S. S.,
Dijkhuizen, L., Vafeiadi, C., & Lamothe, L. (2017).
Branched alpha glucans. (Patent No.
WO2017046040). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2017046040A1&KC=A1&FT=D&ND=3&date=20170323&DB=&locale=en_EP
te
Poele, E. M., Valk, V., Devlamynck, T., van Leeuwen,
S. S., & Dijkhuizen, L. (2017).
Catechol glucosides act as donor/acceptor substrates of
glucansucrase enzymes of Lactobacillus reuteri.
Applied Microbiology and Biotechnology,
101(11), 4495-4505. https://doi.org/10.1007/s00253-017-8190-z
Meng,
X., Pijning, T., Tietema, M., Dobruchowska, J. M.,
Yin, H., Gerwig, G. J., Kralj, S., &
Dijkhuizen, L. (2017). Characterization of the
glucansucrase GTF180 W1065 mutant enzymes producing polysaccharides
and oligosaccharides with altered linkage composition.
Food Chemistry, 217, 81-90. https://doi.org/10.1016/j.foodchem.2016.08.087
Gangoiti,
J., Lamothe, L., van Leeuwen, S. S., Vafiadi,
C., & Dijkhuizen, L. (2017).
Characterization of the Paenibacillus beijingensis DSM 24997
GtfD and its glucan polymer products representing a new glycoside
hydrolase 70 subfamily of 4,6-α-glucanotransferase
enzymes. PLoS ONE, 12(4),
[e0172622]. https://doi.org/10.1371/journal.pone.0172622
Gangoiti
Muñecas, J., Meng, X., Lamerts van Bueren,
A., & Dijkhuizen, L. (2017). Draft
Genome Sequence of Lactobacillus reuteri 121, a Source of
α-Glucan and β-Fructan Exopolysaccharides.
Genome Announcements, 5(10), 1-2.
[e01691-16]. https://doi.org/10.1128/genomeA.01691-16
Yin,
H., Pijning, T., Meng, X., Dijkhuizen,
L., & van Leeuwen, S. S. (2017).
Engineering of the Bacillus circulans β-galactosidase
product specificity. Biochemistry,
56(5), 704-711. https://doi.org/10.1021/acs.biochem.7b00032
Ceniceros,
A., Dijkhuizen, L., Petrusma, M., & Medema, M. H.
(2017). Genome-based exploration of the specialized metabolic
capacities of the genus Rhodococcus. BMC
Genomics, 18(1), 1-16. [593]. https://doi.org/10.1186/s12864-017-3966-1
Gangoiti,
J., van Leeuwen, S. S., Meng, X., Duboux, S.,
Vafiadi, C., Pijning, T., & Dijkhuizen,
L. (2017). Mining novel starch-converting Glycoside
Hydrolase 70 enzymes from the Nestlé Culture Collection genome
database: The Lactobacillus reuteri NCC 2613 GtfB.
Scientific Reports, 7(1), [9947]. https://doi.org/10.1038/s41598-017-07190-z
Frasch,
H-J., Leeuwen, S. S. V., & Dijkhuizen,
L. (2017). Molecular and biochemical characteristics
of the inulosucrase HugO from Streptomyces viridochromogenes
DSM40736 (Tü494).
Microbiology-Reading, 163(7),
1030-1041. https://doi.org/10.1099/mic.0.000493
Ceniceros,
A., Dijkhuizen, L., & Petrusma, M. (2017).
Molecular characterization of a Rhodococcus jostii RHA1
γ-butyrolactone(-like) signalling molecule and its main
biosynthesis gene gblA. Scientific
Reports, 7(1), 1-13. [17743]. https://doi.org/10.1038/s41598-017-17853-6
Lammerts
van Bueren, A., Mulder, M., Leeuwen, S.
V., & Dijkhuizen, L. (2017).
Prebiotic galactooligosaccharides activate mucin and pectic
galactan utilization pathways in the human gut symbiont Bacteroides
thetaiotaomicron. Scientific Reports,
7, 1-13. [40478]. https://doi.org/10.1038/srep40478
Yin,
H., Bultema, J. B., Dijkhuizen, L., & van
Leeuwen, S. S. (2017). Reaction kinetics and
galactooligosaccharide product profiles of the
β-galactosidases from Bacillus circulans, Kluyveromyces lactis
and Aspergillus oryzae. Food
Chemistry, 225, 230-238.
[j.foodchem.2017.01.030]. https://doi.org/10.1016/j.foodchem.2017.01.030
Gerwig,
G. J., Te Poele, E. M., Dijkhuizen,
L., & Kamerling, J. P. (2017). Structural analysis
of rebaudioside A derivatives obtained by Lactobacillus reuteri 180
glucansucrase-catalyzed trans-α-glucosylation.
Carbohydrate Research, 440-441,
51-62. https://doi.org/10.1016/j.carres.2017.01.008
Pham,
H. T. T., Dijkhuizen, L., & van Leeuwen, S.
S. (2017). Structural characterization of glucosylated
lactose derivatives synthesized by the Lactobacillus reuteri GtfA
and Gtf180 glucansucrase enzymes. Carbohydrate
Research, 449, 59-64. https://doi.org/10.1016/j.carres.2017.07.002
Montersino,
S., te Poele, E., Orru, R., Westphal, A. H.,
Barendregt, A., Heck, A. J. R., van der Geize, R.,
Dijkhuizen, L., Mattevi, A., & van Berkel, W. J. H.
(2017). 3-Hydroxybenzoate 6-Hydroxylase from Rhodococcus
jostii RHA1 Contains a Phosphatidylinositol Cofactor.
Frontiers in Microbiology, 8, 1-11.
[1110]. https://doi.org/10.3389/fmicb.2017.01110
Gangoiti
Muñecas, J., van Leeuwen, S. S.,
Gerwig, G. J., Duboux, S., Vafiadi, C., Pijning,
T., & Dijkhuizen, L. (2017).
4,3-α-Glucanotransferase, a novel reaction specificity
in glycoside hydrolase family 70 and clan GH-H.
Scientific Reports, 7, 1-15.
[39761]. https://doi.org/10.1038/srep39761
2016
Bai,
Y., Gangoiti , J., Dijkstra, B. W., Dijkhuizen,
L., & Pijning, T. (2017). Crystal
Structure of 4,6-α-Glucanotransferase Supports Diet-Driven
Evolution of GH70 Enzymes from α-Amylases in Oral
Bacteria. Structure, 25(2),
231-242. https://doi.org/10.1016/j.str.2016.11.023
Valk,
V., Lammerts van Bueren, A., van der Kaaij, R.
M., & Dijkhuizen, L. (2016).
Carbohydrate Binding Module 74 is a novel starch binding
domain associated with large and multi-domain α-amylase
enzymes. Febs Journal,
283(12), 2354-2368. [13745]. https://doi.org/10.1111/febs.13745
Valk,
V., van der Kaaij, R. M., & Dijkhuizen,
L. (2016). Characterization of the starch-acting
MaAmyB enzyme from Microbacterium aurum B8.A representing the novel
subfamily GH13_42 with an unusual, multi-domain
organization. Scientific Reports,
6, 1-12. [srep36100]. https://doi.org/10.1038/srep36100
van
Leeuwen, S. S., Kuipers, B. J. H., Dijkhuizen,
L., & Kamerling, J. P. (2016). Comparative
structural characterization of 7 commercial galacto-oligosaccharide
(GOS) products. Carbohydrate Research,
425, 48-58. https://doi.org/10.1016/j.carres.2016.03.006
van
Leeuwen, S. S., Kuipers, B. J. H., Dijkhuizen,
L., & Kamerling, J. P. (2016). Corrigendum to
“1H NMR analysis of the lactose/β-galactosidase-derived
galacto-oligosaccharide components of Vivinal® GOS up to
DP5” [Carbohydr. Res. 400 (2014) 59–73].
Carbohydrate Research, 419, 69-70.
https://doi.org/10.1016/j.carres.2015.12.004
Pijning,
T., Bai, Y., Gangoiti Muñecas, J.,
& Dijkhuizen, L. (2016). Crystal Structure of
a 4,6-α-Glucanotransferase Supports Diet-Driven Evolution of
GH70 Enzymes from α-Amylases in Oral Bacteria.
Poster session presented at ALAMY, Smolenice, Slovakia.
Figueroa,
S., Dijkhuizen, L., Valk, R., van Leeuwen,
S., & de Vos, P. (2016). Dietary N- and O-glycans
from cow milk and TLR modulation. European Journal
of Immunology, 46, 479-479.
Devlamynck,
T., Te Poele, E. M., Meng, X., van Leeuwen, S.
S., & Dijkhuizen, L. (2016).
Glucansucrase Gtf180-Delta N of Lactobacillus reuteri 180:
enzyme and reaction engineering for improved glycosylation of
non-carbohydrate molecules. Applied Microbiology
and Biotechnology, 100(17), 7529-7539. https://doi.org/10.1007/s00253-016-7476-x
Te
Poele, E. M., Grijpstra, P., van Leeuwen,
S. S., & Dijkhuizen, L. (2016).
Glucosylation of catechol with the GTFA glucansucrase enzyme
from Lactobacillus reuteri and sucrose as donor substrate.
BIOCONJUGATE CHEMISTRY, 27(4),
937-946. https://doi.org/10.1021/acs.bioconjchem.6b00018
Bai,
Y., Böger, M., van der Kaaij, R. M.,
Woortman, A. J. J., Pijning, T., van
Leeuwen, S. S., Lammerts van Bueren, A.,
& Dijkhuizen, L. (2016). Lactobacillus reuteri
strains convert starch and maltodextrins into
homo-exopolysaccharides using an extracellular and cell-associated
4,6-α-glucanotransferase. Journal of
Agricultural and Food Chemistry, 64(14),
2941-2952. https://doi.org/10.1021/acs.jafc.6b00714
te
Poele, E. M., Dijkhuizen, L., Gerwig, G.
J., & Kamerling, J. P. (2016). Methods for the enzymatic modification of steviol
glycosides, modified steviol glycosides obtainable thereby, and the
use thereof as sweeteners. (Patent No.
WO2016144175). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2016144175A1&KC=A1&FT=D&ND=3&date=20160915&DB=EPODOC&locale=en_EP
Dobruchowska,
J. M., Jonsson, J. O., Fridjonsson, O. H., Aevarsson, A.,
Kristjansson, J. K., Altenbuchner, J., Watzlawick, H.,
Gerwig, G. J., Dijkhuizen, L., Kamerling, J.
P., & Hreggvidsson, G. O. (2016). Modification of linear
(β1→3)-linked gluco-oligosaccharides with a novel
recombinant β-glucosyltransferase (trans-β-glucosidase)
enzyme from Bradyrhizobium diazoefficiens.
Glycobiology, 26(11), 1157-1170. https://doi.org/10.1093/glycob/cww074
Gerwig,
G. J., Te Poele, E. M., Dijkhuizen,
L., & Kamerling, J. P. (2016). Stevia Glycosides:
Chemical and Enzymatic Modifications of Their Carbohydrate Moieties
to Improve the Sweet-Tasting Quality. Advances in
carbohydrate chemistry and biochemistry, 73,
1-72. https://doi.org/10.1016/bs.accb.2016.05.001
Bai,
Y., Dobruchowska, J. M., van der Kaaij, R. M.,
Gerwig, G. J., & Dijkhuizen, L. (2016).
Structural basis for the roles of starch and sucrose in
homo-exopolysaccharide formation by Lactobacillus reuteri
35-5. Carbohydrate Polymers,
151, 29-39. https://doi.org/10.1016/j.carbpol.2016.05.048
Meng,
X., Pijning, T., Dobruchowska, J. M., Yin, H.,
Gerwig, G. J., & Dijkhuizen, L. (2016).
Structural determinants of alternating
(α1 → 4) and (α1 → 6)
linkage specificity in reuteransucrase of Lactobacillus
reuteri. Scientific Reports,
6, [35261]. https://doi.org/10.1038/srep35261
Meng,
X., Gangoiti, J., Bai, Y., Pijning, T., Van
Leeuwen, S. S., & Dijkhuizen, L. (2016).
Structure-function relationships of family GH70 glucansucrase
and 4,6-α-glucanotransferase enzymes, and their evolutionary
relationships with family GH13 enzymes. Cellular
and molecular life sciences, 73(14),
2681-2706. https://doi.org/10.1007/s00018-016-2245-7
Gangoiti,
J., van Leeuwen, S. S., Vafiadi, C., &
Dijkhuizen, L. (2016). The gram-negative bacterium
Azotobacter chroococcum NCIMB 8003 employs a new glycoside
hydrolase family 70 4,6-α-glucanotransferase enzyme (GtfD) to
synthesize a reuteran like polymer from maltodextrins and
starch. Biochimica et biophysica acta,
1860(6), 1224-1236. https://doi.org/10.1016/j.bbagen.2016.02.005
2015
Meng,
X., Dobruchowska, J. M., Pijning, T., Gerwig, G.
J., & Dijkhuizen, L. (2016).
Synthesis of New Hyper-Branched α-Glucans from Sucrose
by Lactobacillus reuteri 180 Glucansucrase Mutants.
Journal of Agricultural and Food Chemistry,
64(2), 433-442. https://doi.org/10.1021/acs.jafc.5b05161
Paul,
C. J., Leemhuis, H., Dobruchowska, J. M., Grey, C., Onnby,
L., van Leeuwen, S. S., Dijkhuizen, L.,
& Karlsson, E. N. (2015). A GH57
4-alpha-glucanotransferase of hyperthermophilic origin with
potential for alkyl glycoside production. Applied
Microbiology and Biotechnology, 99(17),
7101-7113. https://doi.org/10.1007/s00253-015-6435-2
Bai,
Y., van der Kaaij, R. M., Leemhuis, H., Pijning,
T., van Leeuwen, S. S., Jin, Z., &
Dijkhuizen, L. (2015). Biochemical characterization of
Lactobacillus reuteri Glycoside Hydrolase family 70 GTFB type of
4,6-α-Glucanotransferase enzymes that synthesize soluble
dietary starch fibers. Applied and environmental
microbiology, 81(20), 7223-7232. https://doi.org/10.1128/AEM.01860-15
Meng,
X., Pijning, T., Dobruchowska, J. M., Gerwig, G.
J., & Dijkhuizen, L. (2015).
Characterization of the functional roles of amino acid
residues in acceptor binding subsite +1 in the active site of the
glucansucrase GTF180 enzyme of Lactobacillus reuteri 180.
The Journal of Biological Chemistry,
290(50), 30131-30141. https://doi.org/10.1074/jbc.M115.687558
van
Munster, J. M., Dobruchowska, J. M., Veloo, R., Dijkhuizen,
L., & van der Maarel, M. J. E. C. (2015).
Characterization of the starvation-induced chitinase CfcA and
α-1,3-glucanase AgnB of Aspergillus niger.
Applied Microbiology and Biotechnology,
99, 2209-2223. https://doi.org/10.1007/s00253-014-6062-3
Bai,
Y., van der Kaaij, R. M., Woortman, A. J.
J., Jin, Z., & Dijkhuizen, L. (2015).
Characterization of the 4,6-α-glucanotransferase GTFB
enzyme of Lactobacillus reuteri 121 isolated from inclusion
bodies. BMC Biotechnology,
15, [49]. https://doi.org/10.1186/s12896-015-0163-7
Valk,
V., Eeuwema, W., Sarian, F. D., van der Kaaij, R.
M., & Dijkhuizen, L. (2015).
Degradation of granular starch by the bacterium
Microbacterium aurum B8.A involves a novel modular α-amylase
enzyme system with FNIII and CBM25 domains.
Applied and environmental microbiology,
81(19), 6610-6620. https://doi.org/10.1128/AEM.01029-15
Lammerts
van Bueren, A., Saraf, A., Martens, E. C., &
Dijkhuizen, L. (2015). Differential metabolism of
Exopolysaccharides from probiotic Lactobacilli by the human gut
symbiont Bacteroides thetaiotaomicron. Applied and
environmental microbiology, 81(12), 3973-3983.
https://doi.org/10.1128/AEM.00149-15
Wilbrink,
M. H., Ten Kate, G. A., Sanders, P., Gerwig, G.
J., van Leeuwen, S. S., Sallomons, E.,
Klarenbeek, B., Hage, J. H., van Vuure, C. A., Dijkhuizen,
L., & Kamerling, J. P. (2015). Enzymatic
Decoration of Prebiotic Galacto-oligosaccharides (Vivinal®
GOS) with Sialic Acid using Trypanosoma cruzi Trans-Sialidase and
Two Bovine Sialoglycoconjugates as Donor Substrates.
Journal of Agricultural and Food Chemistry,
63(25), 5976-5984. https://doi.org/10.1021/acs.jafc.5b01505
van
Munster, J. M., Sanders, P., ten Kate, G. A.,
Dijkhuizen, L., & van der Maarel, M. J. E.
C. (2015). Kinetic characterization of Aspergillus
niger chitinase CfcI using a HPAEC-PAD method for native chitin
oligosaccharides. Carbohydrate
Research, 407, 73-78. https://doi.org/10.1016/j.carres.2015.01.014
Aalbers,
F., Turkenburg, J. P., Davies, G. J., Dijkhuizen,
L., & Lammerts van Bueren, A. (2015).
Structural and Functional Characterization of a Novel Family
GH115 4-O-Methyl-α-Glucuronidase with Specificity for
Decorated Arabinogalactans. Journal of Molecular
Biology, 427(24), 3935-3946. https://doi.org/10.1016/j.jmb.2015.07.006
Meng,
X., Dobruchowska, J. M., Gerwig, G. J., Kamerling, J.
P., & Dijkhuizen, L. (2015). Synthesis of
oligo- and polysaccharides by Lactobacillus reuteri 121
reuteransucrase at high concentrations of sucrose.
Carbohydrate Research, 414, 85-92.
https://doi.org/10.1016/j.carres.2015.07.011
van
Munster, J. M., Nitsche, B. M., Akeroyd, M., Dijkhuizen,
L., van der Maarel, M. J. E. C., & Ram, A.
F. J. (2015). Systems Approaches to Predict the Functions of
Glycoside Hydrolases during the Life Cycle of Aspergillus niger
Using Developmental Mutants ∆brlA and ∆flbA.
PLoS ONE, 10(1), [e0116269]. https://doi.org/10.1371/journal.pone.0116269
Gangoiti,
J., Pijning, T., & Dijkhuizen, L.
(2015). The Exiguobacterium sibiricum 255-15 GtfC enzyme
representing a novel glycoside hydrolase 70 subfamily of
4,6-α-glucanotransferase enzymes. Applied and
environmental microbiology, 82(2), 756-766.
[AEM.03420-15]. https://doi.org/10.1128/AEM.03420-15
Meng,
X., Dobruchowska, J. M., Pijning, T., Gerwig, G.
J., Kamerling, J. P., & Dijkhuizen, L.
(2015). Truncation of domain V of the multidomain
glucansucrase GTF180 of Lactobacillus reuteri 180 heavily impairs
its polysaccharide-synthesizing ability. Applied
Microbiology and Biotechnology, 99(14),
5885-5894. https://doi.org/10.1007/s00253-014-6361-8
2014
Venkataraman,
H., Te Poele, E. M., Rosłoniec, K. Z., Vermeulen,
N., Commandeur, J. N. M., van der Geize, R., &
Dijkhuizen, L. (2015). Biosynthesis of a steroid
metabolite by an engineered Rhodococcus erythropolis strain
expressing a mutant cytochrome P450 BM3 enzyme.
Applied Microbiology and Biotechnology,
99(11), 4713-4721. https://doi.org/10.1007/s00253-014-6281-7
Bultema,
J. B., Kuipers, B. J. H., & Dijkhuizen, L. (2014).
Biochemical characterization of mutants in the active site
residues of the beta-galactosidase enzyme of Bacillus circulans
ATCC 31382. FEBS Open Bio,
4(1), 1015-1020. https://doi.org/10.1016/j.fob.2014.11.002
van
Leeuwen, S. S., Kuipers, B. J. H., Dijkhuizen,
L., & Kamerling, J. P. (2014). Development of a
H-1 NMR structural-reporter-group concept for the analysis of
prebiotic galacto-oligosaccharides of the [beta-D-Galp-(1 ->
x)](n)-D-Glcp type. Carbohydrate
Research, 400, 54-58. https://doi.org/10.1016/j.carres.2014.08.011
Pijning,
T., Vujicic - Zagar, A., Kralj, S., Dijkhuizen,
L., & Dijkstra, B. W. (2014). Flexibility of
truncated and full-length glucansucrase GTF180 enzymes from
Lactobacillus reuteri 180. Febs
Journal, 281(9), 2159-2171. https://doi.org/10.1111/febs.12769
Wilbrink,
M. H., ten Kate, G. A., van Leeuwen, S. S.,
Sanders, P., Sallomons, E., Hage, J. A., Dijkhuizen,
L., & Kamerling, J. P. (2014). Galactosyl-Lactose
Sialylation Using Trypanosoma cruzi trans-Sialidase as the
Biocatalyst and Bovine kappa-Casein-Derived Glycomacropeptide as
the Donor Substrate. Applied and environmental
microbiology, 80(19), 5984-5991. https://doi.org/10.1128/AEM.01465-14
Jonsson
Wheat, J. O., Hreggvidsson, G. O., Fridjonsson, O. H.,
Dobruchowska, J. M., Kamerling, J. P., & Dijkhuizen,
L. (2014). Glucan branching enzymes and
their methods of use. (Patent No. Netherlands). https://nl.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2014155395A2&KC=A2&FT=D&ND=3&date=20141002&DB=&locale=nl_NL
Hill,
P., Piel, J., Aris‑Brosou, S., Krištůfek, V.,
Boddy, C. N., & Dijkhuizen, L. (2014).
Habitat‑specific type I polyketide synthases in soils
and street sediments. Journal of Industrial
Microbiology & Biotechnology, 41(1),
75-85. https://doi.org/10.1007/s10295-013-1362-7
van
Leeuwen, S. S., Kuipers, B. J. H., Dijkhuizen,
L., & Kamerling, J. P. (2014). H-1 NMR analysis of
the lactose/beta-galactosidase-derived galacto-oligosaccharide
components of Vivinal (R) GOS up to DP5.
Carbohydrate Research, 400, 59-73.
https://doi.org/10.1016/j.carres.2014.08.012
Leemhuis,
H., Dobruchowska, J. M., Ebbelaar, M., Faber, F., Buwalda, P.
L., van der Maarel, M. J. E. C., Kamerling, J.
P., & Dijkhuizen, L. (2014).
Isomalto/Malto-polysaccharide, a novel soluble dietary fiber
made via enzymatic conversion of starch. Journal
of Agricultural and Food Chemistry, 62(49),
12034-12044. https://doi.org/10.1021/jf503970a
van
Leeuwen, S. S., Schoemaker, R. J. W., Gerwig, G.
J., van Leusen-van Kan, E. J. M., Dijkhuizen,
L., & Kamerling, J. P. (2014). Rapid milk group
classification by 1H NMR analysis of Le and H epitopes in human
milk oligosaccharide donor samples.
Glycobiology, 24(8), 728-739. https://doi.org/10.1093/glycob/cwu036
Meng,
X., Dobruchowska, J. M., Pijning, T., Lopez-Bautista,
C., Kamerling, J. P., & Dijkhuizen, L. (2014).
Residue Leu(940) Has a Crucial Role in the Linkage and
Reaction Specificity of the Glucansucrase GTF180 of the Probiotic
Bacterium Lactobacillus reuteri 180. The Journal
of Biological Chemistry, 289(47), 32773-32782.
https://doi.org/10.1074/jbc.M114.602524
Petrusma,
M., van der Geize, R., & Dijkhuizen, L. (2014).
3-Ketosteroid 9 alpha-hydroxylase enzymes: Rieske non-heme
monooxygenases essential for bacterial steroid degradation.
Antonie Van Leeuwenhoek: International Journal of General
and Molecular Microbiology, 106(1), 157-172.
https://doi.org/10.1007/s10482-014-0188-2
2013
Timm,
M., Goerl, J., Kraus, M., Kralj, S., Hellmuth, H., Beine, R.,
Buchholz, K., Dijkhuizen, L., Seibel, J., &
Seibel, J. (2013). An Unconventional Glycosyl Transfer
Reaction: Glucansucrase GTFA Functions as an Allosyltransferase
Enzyme. ChemBioChem, 14(18),
2423-2426. https://doi.org/10.1002/cbic.201300392
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