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Dijkhuizen, Prof. Lubbert

Lubbert Dijkhuizen
Lubbert Dijkhuizen

Lubbert Dijkhuizen (1951) is professor of Microbiology. He initiated many research projects into life processes in micro-organisms and relevant industrial and other applications of this fundamental knowledge. The main focus in this research is on the ability to use micro-organisms and enzymes in biotechnological processes, in particular in the conversion of carbohydrates. Dijkhuizen's research also focuses on tuberculosis.

Dijkhuizen is one of the initiators and joint founders of the Carbohydrate Competence Center (CCC), a public-private cooperation among 19 businesses and 6 knowledge institutions. Research is being conducted in 24 projects (total value € 28 million) on carbohydrates that play an important role in Nutrition and Health. The Dagblad van het Noorden has written an overview article about the CCC's research and its implications for healthy nutrition, for example.

Dijkhuizen has thus far supervised over 55 PhD students and written over 300 international scientific publications. His research is internationally renowned and is so fruitful that he is always able to acquire the necessary funds. He has a good eye for the possible products and processes that his research may yield. This has resulted, for example, in his being mentioned as the inventor or joint inventor in 25 patents and about 75% of his research group being financed by external funds, mainly by industrial parties. In late 2010, Dijkhuizen and his colleague Dijkstra caused an international stir with an article in PNAS about a possible solution to prevent caries. In 2012 Dijkhuizen was nominated as one of the three most enterprising researchers of The Netherlands. the same year, Dijkhuizen's CCC announced a breakthrough in carbohydrate research, as a result of which, among other things, French Fries can be made healthier.

Previously in the news

UG article
UG article

Publications

2021

Valk-Weeber, R. L., Nichols, K., Dijkhuizen, L., Bijl, E., & van Leeuwen, S. S. (2021). Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects. Journal of dairy science, 104(4), 5056-5068. https://doi.org/10.3168/jds.2020-19297

2020

Hartman, A. M., Jumde, V. R., Elgaher, W. A. M., Te Poele, E. M., Dijkhuizen, L., & Hirsch, A. K. H. (2021). Potential Dental Biofilm Inhibitors: Dynamic Combinatorial Chemistry Affords Sugar-Based Molecules that Target Bacterial Glucosyltransferase. ChemMedChem, 16(1), 113-123. [cmdc.202000222]. https://doi.org/10.1002/cmdc.202000222
Te Poele, E., Corwin, S., Hamaker, B. R., Lamothe, L., Vafeiadi, C., & Dijkhuizen, L. (2020). Development of Slowly Digestible Starch Derived α-Glucans with 4,6-α-Glucanotransferase and Branching Sucrase Enzymes. Journal of Agricultural and Food Chemistry, 68(24), 6664-6671. [acs.jafc.0c01465]. https://doi.org/10.1021/acs.jafc.0c01465
Knight, J., Branneby, C. K., Dijkhuizen, L., Petrusma, M., & Fernández de las Heras, L. (2020). Genetically-modified bacteria and uses thereof. (Patent No. WO2020030799).
van Leeuwen, S. S., Te Poele, E. M., Chatziioannou, A. C., Benjamins, E., Haandrikman, A., & Dijkhuizen, L. (2020). Goat Milk Oligosaccharides: Their Diversity, Quantity, and Functional Properties in Comparison to Human Milk Oligosaccharides. Journal of Agricultural and Food Chemistry, 68(47), 13469-13485. https://doi.org/10.1021/acs.jafc.0c03766
Valk-Weeber, R. L., Deelman-Driessen, C., Dijkhuizen, L., Eshuis-de Ruiter, T., & van Leeuwen, S. (2020). In depth analysis of the contribution of specific glycoproteins to the overall bovine whey N-linked glycoprofile. Journal of Agricultural and Food Chemistry, 68(24), 6544-6553. [acs.jafc.0c00959]. https://doi.org/10.1021/acs.jafc.0c00959
Figueroa-Lozano, S., Valk-Weeber, R. L., Akkerman, R., Abdulahad, W., van Leeuwen, S. S., Dijkhuizen, L., & de Vos, P. (2020). Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine. Frontiers in Immunology, 11, [790]. https://doi.org/10.3389/fimmu.2020.00790
Valk-Weeber, R. L., Eshuis-de Ruiter, T., Dijkhuizen, L., & van Leeuwen, S. S. (2020). Quantitative analysis of bovine whey glycoproteins using the overall N-linked whey glycoprofile. International Dairy Journal, 110, [104814]. https://doi.org/10.1016/j.idairyj.2020.104814
Kittibunchakul, S., van Leeuwen, S. S., Dijkhuizen, L., Haltrich, D., & Thu-Ha Nguyen (2020). Structural Comparison of Different Galacto-oligosaccharide Mixtures Formed by beta-Galactosidases from Lactic Acid Bacteria and Bifidobacteria. Journal of Agricultural and Food Chemistry, 68(15), 4437-4446. https://doi.org/10.1021/acs.jafc.9b08156
Li, X., Wang, X., Meng, X., Dijkhuizen, L., & Liu, W. (2020). Structures, physico-chemical properties, production and (potential) applications of sucrose-derived α-d-glucans synthesized by glucansucrases. Carbohydrate Polymers, 249, [116818]. https://doi.org/10.1016/j.carbpol.2020.116818
Figueroa-Lozano, S., Ren, C., Yin, H., Pham, H., van Leeuwen, S., Dijkhuizen, L., & de Vos, P. (2020). The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells. Food & Function, 11(4), 3506-3515. https://doi.org/10.1039/d0fo00064g

2019

Valk-Weeber, R. L., Eshuis-de Ruiter, T., Dijkhuizen, L., & van Leeuwen, S. S. (2020). Dynamic temporal variations in bovine lactoferrin glycan structures. Journal of Agricultural and Food Chemistry, 68(2), 549-560. [acs.jafc.9b06762]. https://doi.org/10.1021/acs.jafc.9b06762
Valk-Weeber, R. L., Dijkhuizen, L., & van Leeuwen, S. S. (2019). Large-scale quantitative isolation of pure protein N-linked glycans. Carbohydrate Research, 479, 13-22. https://doi.org/10.1016/j.carres.2019.04.011
Böger, M., Hekelaar, J., van Leeuwen, S. S., Dijkhuizen, L., & Lammerts van Bueren, A. (2019). Structural and functional characterization of a family GH53 β-1,4-galactanase from Bacteroides thetaiotaomicron that facilitates degradation of prebiotic galactooligosaccharides. Journal of Structural Biology, 205(1), 1-10. [j.jsb.2018.12.002]. https://doi.org/10.1016/j.jsb.2018.12.002
Boger, M., van Leeuwen, S. S., van Bueren, A. L., & Dijkhuizen, L. (2019). Structural Identity of Galactooligosaccharide Molecules Selectively Utilized by Single Cultures of Probiotic Bacterial Strains. Journal of Agricultural and Food Chemistry, 67(50), 13969-13977. https://doi.org/10.1021/acs.jafc.9b05968
Pham, H., Ten Kate, G. A., Dijkhuizen, L., & van Leeuwen, S. S. (2019). Synthesis and characterization of sialylated lactose- and lactulose-derived oligosaccharides by Trypanosoma cruzi trans-sialidase. Journal of Agricultural and Food Chemistry, 67(12), 3469–3479. https://doi.org/10.1021/acs.jafc.8b06974
Devlamynck, T., Te Poele, E. M., Quataert, K., Gerwig, G. J., Van de Walle, D., Dewettinck, K., Kamerling, J. P., Soetaert, W., & Dijkhuizen, L. (2019). Trans-α-glucosylation of stevioside by the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E improves its taste profile. Food Chemistry, 272, 653-662. https://doi.org/10.1016/j.foodchem.2018.08.025

2018

Gangoiti, J., Corwin, S. F., Lamothe, L. M., Vafiadi, C., Hamaker, B. R., & Dijkhuizen, L. (2020). Synthesis of novel α-glucans with potential health benefits through controlled glucose release in the human gastrointestinal tract. Critical Reviews in Food Science and Nutrition, 60(1), 123-146. https://doi.org/10.1080/10408398.2018.1516621
Pham, H. T. T., Boger, M. C. L., Dijkhuizen, L., & van Leeuwen, S. S. (2019). Stimulatory effects of novel glucosylated lactose derivatives GL34 on growth of selected gut bacteria. Applied Microbiology and Biotechnology, 103(2), 707-718. https://doi.org/10.1007/s00253-018-9473-8
Meng, X., Gangoiti, J., Wang, X., Grijpstra, P., van Leeuwen, S. S., Pijning, T., & Dijkhuizen, L. (2018). Biochemical characterization of a GH70 protein from Lactobacillus kunkeei DSM 12361 with two catalytic domains involving branching sucrase activity. Applied Microbiology and Biotechnology, 102(18), 7935-7950. https://doi.org/10.1007/s00253-018-9236-6
Meng, X., Gangoiti, J., de Kok, N., van Leeuwen, S. S., Pijning, T., & Dijkhuizen, L. (2018). Biochemical characterization of two GH70 family 4,6-α-glucanotransferases with distinct product specificity from Lactobacillus aviarius subsp. aviarius DSM 20655. Food Chemistry, 253, 236-246. [j.foodchem.2018.01.154]. https://doi.org/10.1016/j.foodchem.2018.01.154
Gangoiti Muñecas, J., van Leeuwen, S. S., Pijning, T., Dijkhuizen, L., Vafeiadi, C., & Duboux, S. (2018). Branched alpha glucans. (Patent No. WO2018167032). https://nl.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2018167032A1&KC=A1&FT=D&ND=3&date=20180920&DB=&locale=nl_NL
Boger, M. C. L., van Bueren, A. L., & Dijkhuizen, L. (2018). Cross-Feeding among Probiotic Bacterial Strains on Prebiotic Inulin Involves the Extracellular exo-Inulinase of Lactobacillus paracasei Strain W20. Applied and environmental microbiology, 84(21), [e01539-18]. https://doi.org/10.1128/AEM.01539-18
Te Poele, E. M., Devlamynck, T., Jäger, M., Gerwig, G. J., Van de Walle, D., Dewettinck, K., Hirsch, A. K. H., Kamerling, J. P., Soetaert, W., & Dijkhuizen, L. (2018). Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180 efficiently transglucosylate Stevia component rebaudioside A, resulting in a superior taste. Scientific Reports, 8(1), [1516]. https://doi.org/10.1038/s41598-018-19622-5
Pham, H., Pijning, T., Dijkhuizen, L., & van Leeuwen, S. S. (2018). Mutational Analysis of the Role of the Glucansucrase Gtf180-Delta N Active Site Residues in Product and Linkage Specificity with Lactose as Acceptor Substrate. Journal of Agricultural and Food Chemistry, 66(47), 12544-12554. [acs.jafc.8b04486]. https://doi.org/10.1021/acs.jafc.8b04486
Lammerts van Bueren-Brandt, A., & Dijkhuizen, L. (2018). Prebiotic branched galacto-oligosaccharides (gos). (Patent No. WO2018048305). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2018048305A1&KC=A1&FT=D&ND=3&date=20180315&DB=&locale=en_EP
van Leeuwen, S. S., Stoutjesdijk, E., ten Kate, G. A., Schaafsma, A., Dijck-Brouwer, J., Muskiet, F. A. J., & Dijkhuizen, L. (2018). Regional variations in human milk oligosaccharides in Vietnam suggest FucTx activity besides FucT2 and FucT3. Scientific Reports, 8(1), [16790]. https://doi.org/10.1038/s41598-018-34882-x
Pham, H. T., Dijkhuizen, L., & van Leeuwen, S. S. (2018). Structural characterization of glucosylated GOS derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes. Carbohydrate Research, 470, 57-63. https://doi.org/10.1016/j.carres.2018.10.003
Yin, H., Dijkhuizen, L., & van Leeuwen, S. (2018). Synthesis of galacto-oligosaccharides derived from lactulose by wild-type and mutant β-galactosidase enzymes from Bacillus circulans ATCC 31382. Carbohydrate Research, 465, 58-65. [j.carres.2018.06.009]. https://doi.org/10.1016/j.carres.2018.06.009

2017

Gangoiti, J., Pijning, T., & Dijkhuizen, L. (2018). Biotechnological potential of novel glycoside hydrolase family 70 enzymes synthesizing α-glucans from starch and sucrose. Biotechnology Advances, 36, 196-207. https://doi.org/10.1016/j.biotechadv.2017.11.001
Figueroa-Lozano, S., Valk-Weeber, R. L., van Leeuwen, S. S., Dijkhuizen, L., & de Vos, P. (2018). Dietary N-glycans from Bovine Lactoferrin and TLR Modulation. Molecular Nutrition & Food Research, 62(2), [1700389]. https://doi.org/10.1002/mnfr.201700389
Sarian, F. D., Janeček, Š., Pijning, T., Ihsanawati, Nurachman, Z., Radjasa, O. K., Dijkhuizen, L., Natalia, D., & van der Maarel, M. J. E. C. (2017). A new group of glycoside hydrolase family 13 α-amylases with an aberrant catalytic triad. Scientific Reports, 7, [44230]. https://doi.org/10.1038/srep44230
Dijkhuizen, L., Gangoiti Muñecas, J., van Leeuwen, S. S., Vafeiadi, C., & Duboux, S. (2017). Alpha glucans. (Patent No. WO2017207663). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2017207663A1&KC=A1&FT=D&ND=3&date=20171207&DB=&locale=en_EP
Yin, H., Pijning, T., Meng, X., Dijkhuizen, L., & van Leeuwen, S. S. (2017). Biochemical characterization of the functional roles of residues in the active site of the β-galactosidase from Bacillus circulans ATCC 31382. Biochemistry, 56(24), 3109-3118. https://doi.org/10.1021/acs.biochem.7b00207
Gangoiti Muñecas, J., van Leeuwen, S. S., Dijkhuizen, L., Vafeiadi, C., & Lamothe, L. (2017). Branched alpha glucans. (Patent No. WO2017046040). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2017046040A1&KC=A1&FT=D&ND=3&date=20170323&DB=&locale=en_EP
te Poele, E. M., Valk, V., Devlamynck, T., van Leeuwen, S. S., & Dijkhuizen, L. (2017). Catechol glucosides act as donor/acceptor substrates of glucansucrase enzymes of Lactobacillus reuteri. Applied Microbiology and Biotechnology, 101(11), 4495-4505. https://doi.org/10.1007/s00253-017-8190-z
Meng, X., Pijning, T., Tietema, M., Dobruchowska, J. M., Yin, H., Gerwig, G. J., Kralj, S., & Dijkhuizen, L. (2017). Characterization of the glucansucrase GTF180 W1065 mutant enzymes producing polysaccharides and oligosaccharides with altered linkage composition. Food Chemistry, 217, 81-90. https://doi.org/10.1016/j.foodchem.2016.08.087
Gangoiti, J., Lamothe, L., van Leeuwen, S. S., Vafiadi, C., & Dijkhuizen, L. (2017). Characterization of the Paenibacillus beijingensis DSM 24997 GtfD and its glucan polymer products representing a new glycoside hydrolase 70 subfamily of 4,6-α-glucanotransferase enzymes. PLoS ONE, 12(4), [e0172622]. https://doi.org/10.1371/journal.pone.0172622
Gangoiti Muñecas, J., Meng, X., Lamerts van Bueren, A., & Dijkhuizen, L. (2017). Draft Genome Sequence of Lactobacillus reuteri 121, a Source of α-Glucan and β-Fructan Exopolysaccharides. Genome Announcements, 5(10), 1-2. [e01691-16]. https://doi.org/10.1128/genomeA.01691-16
Yin, H., Pijning, T., Meng, X., Dijkhuizen, L., & van Leeuwen, S. S. (2017). Engineering of the Bacillus circulans β-galactosidase product specificity. Biochemistry, 56(5), 704-711. https://doi.org/10.1021/acs.biochem.7b00032
Ceniceros, A., Dijkhuizen, L., Petrusma, M., & Medema, M. H. (2017). Genome-based exploration of the specialized metabolic capacities of the genus Rhodococcus. BMC Genomics, 18(1), 1-16. [593]. https://doi.org/10.1186/s12864-017-3966-1
Gangoiti, J., van Leeuwen, S. S., Meng, X., Duboux, S., Vafiadi, C., Pijning, T., & Dijkhuizen, L. (2017). Mining novel starch-converting Glycoside Hydrolase 70 enzymes from the Nestlé Culture Collection genome database: The Lactobacillus reuteri NCC 2613 GtfB. Scientific Reports, 7(1), [9947]. https://doi.org/10.1038/s41598-017-07190-z
Frasch, H-J., Leeuwen, S. S. V., & Dijkhuizen, L. (2017). Molecular and biochemical characteristics of the inulosucrase HugO from Streptomyces viridochromogenes DSM40736 (Tü494). Microbiology-Reading, 163(7), 1030-1041. https://doi.org/10.1099/mic.0.000493
Ceniceros, A., Dijkhuizen, L., & Petrusma, M. (2017). Molecular characterization of a Rhodococcus jostii RHA1 γ-butyrolactone(-like) signalling molecule and its main biosynthesis gene gblA. Scientific Reports, 7(1), 1-13. [17743]. https://doi.org/10.1038/s41598-017-17853-6
Lammerts van Bueren, A., Mulder, M., Leeuwen, S. V., & Dijkhuizen, L. (2017). Prebiotic galactooligosaccharides activate mucin and pectic galactan utilization pathways in the human gut symbiont Bacteroides thetaiotaomicron. Scientific Reports, 7, 1-13. [40478]. https://doi.org/10.1038/srep40478
Yin, H., Bultema, J. B., Dijkhuizen, L., & van Leeuwen, S. S. (2017). Reaction kinetics and galactooligosaccharide product profiles of the β-galactosidases from Bacillus circulans, Kluyveromyces lactis and Aspergillus oryzae. Food Chemistry, 225, 230-238. [j.foodchem.2017.01.030]. https://doi.org/10.1016/j.foodchem.2017.01.030
Gerwig, G. J., Te Poele, E. M., Dijkhuizen, L., & Kamerling, J. P. (2017). Structural analysis of rebaudioside A derivatives obtained by Lactobacillus reuteri 180 glucansucrase-catalyzed trans-α-glucosylation. Carbohydrate Research, 440-441, 51-62. https://doi.org/10.1016/j.carres.2017.01.008
Pham, H. T. T., Dijkhuizen, L., & van Leeuwen, S. S. (2017). Structural characterization of glucosylated lactose derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes. Carbohydrate Research, 449, 59-64. https://doi.org/10.1016/j.carres.2017.07.002
Montersino, S., te Poele, E., Orru, R., Westphal, A. H., Barendregt, A., Heck, A. J. R., van der Geize, R., Dijkhuizen, L., Mattevi, A., & van Berkel, W. J. H. (2017). 3-Hydroxybenzoate 6-Hydroxylase from Rhodococcus jostii RHA1 Contains a Phosphatidylinositol Cofactor. Frontiers in Microbiology, 8, 1-11. [1110]. https://doi.org/10.3389/fmicb.2017.01110
Gangoiti Muñecas, J., van Leeuwen, S. S., Gerwig, G. J., Duboux, S., Vafiadi, C., Pijning, T., & Dijkhuizen, L. (2017). 4,3-α-Glucanotransferase, a novel reaction specificity in glycoside hydrolase family 70 and clan GH-H. Scientific Reports, 7, 1-15. [39761]. https://doi.org/10.1038/srep39761

2016

Bai, Y., Gangoiti , J., Dijkstra, B. W., Dijkhuizen, L., & Pijning, T. (2017). Crystal Structure of 4,6-α-Glucanotransferase Supports Diet-Driven Evolution of GH70 Enzymes from α-Amylases in Oral Bacteria. Structure, 25(2), 231-242. https://doi.org/10.1016/j.str.2016.11.023
Valk, V., Lammerts van Bueren, A., van der Kaaij, R. M., & Dijkhuizen, L. (2016). Carbohydrate Binding Module 74 is a novel starch binding domain associated with large and multi-domain α-amylase enzymes. Febs Journal, 283(12), 2354-2368. [13745]. https://doi.org/10.1111/febs.13745
Valk, V., van der Kaaij, R. M., & Dijkhuizen, L. (2016). Characterization of the starch-acting MaAmyB enzyme from Microbacterium aurum B8.A representing the novel subfamily GH13_42 with an unusual, multi-domain organization. Scientific Reports, 6, 1-12. [srep36100]. https://doi.org/10.1038/srep36100
van Leeuwen, S. S., Kuipers, B. J. H., Dijkhuizen, L., & Kamerling, J. P. (2016). Comparative structural characterization of 7 commercial galacto-oligosaccharide (GOS) products. Carbohydrate Research, 425, 48-58. https://doi.org/10.1016/j.carres.2016.03.006
van Leeuwen, S. S., Kuipers, B. J. H., Dijkhuizen, L., & Kamerling, J. P. (2016). Corrigendum to “1H NMR analysis of the lactose/β-galactosidase-derived galacto-oligosaccharide components of Vivinal® GOS up to DP5” [Carbohydr. Res. 400 (2014) 59–73]. Carbohydrate Research, 419, 69-70. https://doi.org/10.1016/j.carres.2015.12.004
Pijning, T., Bai, Y., Gangoiti Muñecas, J., & Dijkhuizen, L. (2016). Crystal Structure of a 4,6-α-Glucanotransferase Supports Diet-Driven Evolution of GH70 Enzymes from α-Amylases in Oral Bacteria. Poster session presented at ALAMY, Smolenice, Slovakia.
Figueroa, S., Dijkhuizen, L., Valk, R., van Leeuwen, S., & de Vos, P. (2016). Dietary N- and O-glycans from cow milk and TLR modulation. European Journal of Immunology, 46, 479-479.
Devlamynck, T., Te Poele, E. M., Meng, X., van Leeuwen, S. S., & Dijkhuizen, L. (2016). Glucansucrase Gtf180-Delta N of Lactobacillus reuteri 180: enzyme and reaction engineering for improved glycosylation of non-carbohydrate molecules. Applied Microbiology and Biotechnology, 100(17), 7529-7539. https://doi.org/10.1007/s00253-016-7476-x
Te Poele, E. M., Grijpstra, P., van Leeuwen, S. S., & Dijkhuizen, L. (2016). Glucosylation of catechol with the GTFA glucansucrase enzyme from Lactobacillus reuteri and sucrose as donor substrate. BIOCONJUGATE CHEMISTRY, 27(4), 937-946. https://doi.org/10.1021/acs.bioconjchem.6b00018
Bai, Y., Böger, M., van der Kaaij, R. M., Woortman, A. J. J., Pijning, T., van Leeuwen, S. S., Lammerts van Bueren, A., & Dijkhuizen, L. (2016). Lactobacillus reuteri strains convert starch and maltodextrins into homo-exopolysaccharides using an extracellular and cell-associated 4,6-α-glucanotransferase. Journal of Agricultural and Food Chemistry, 64(14), 2941-2952. https://doi.org/10.1021/acs.jafc.6b00714
te Poele, E. M., Dijkhuizen, L., Gerwig, G. J., & Kamerling, J. P. (2016). Methods for the enzymatic modification of steviol glycosides, modified steviol glycosides obtainable thereby, and the use thereof as sweeteners. (Patent No. WO2016144175). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2016144175A1&KC=A1&FT=D&ND=3&date=20160915&DB=EPODOC&locale=en_EP
Dobruchowska, J. M., Jonsson, J. O., Fridjonsson, O. H., Aevarsson, A., Kristjansson, J. K., Altenbuchner, J., Watzlawick, H., Gerwig, G. J., Dijkhuizen, L., Kamerling, J. P., & Hreggvidsson, G. O. (2016). Modification of linear (β1→3)-linked gluco-oligosaccharides with a novel recombinant β-glucosyltransferase (trans-β-glucosidase) enzyme from Bradyrhizobium diazoefficiens. Glycobiology, 26(11), 1157-1170. https://doi.org/10.1093/glycob/cww074
Gerwig, G. J., Te Poele, E. M., Dijkhuizen, L., & Kamerling, J. P. (2016). Stevia Glycosides: Chemical and Enzymatic Modifications of Their Carbohydrate Moieties to Improve the Sweet-Tasting Quality. Advances in carbohydrate chemistry and biochemistry, 73, 1-72. https://doi.org/10.1016/bs.accb.2016.05.001
Bai, Y., Dobruchowska, J. M., van der Kaaij, R. M., Gerwig, G. J., & Dijkhuizen, L. (2016). Structural basis for the roles of starch and sucrose in homo-exopolysaccharide formation by Lactobacillus reuteri 35-5. Carbohydrate Polymers, 151, 29-39. https://doi.org/10.1016/j.carbpol.2016.05.048
Meng, X., Pijning, T., Dobruchowska, J. M., Yin, H., Gerwig, G. J., & Dijkhuizen, L. (2016). Structural determinants of alternating (α1 → 4) and (α1 → 6) linkage specificity in reuteransucrase of Lactobacillus reuteri. Scientific Reports, 6, [35261]. https://doi.org/10.1038/srep35261
Meng, X., Gangoiti, J., Bai, Y., Pijning, T., Van Leeuwen, S. S., & Dijkhuizen, L. (2016). Structure-function relationships of family GH70 glucansucrase and 4,6-α-glucanotransferase enzymes, and their evolutionary relationships with family GH13 enzymes. Cellular and molecular life sciences, 73(14), 2681-2706. https://doi.org/10.1007/s00018-016-2245-7
Gangoiti, J., van Leeuwen, S. S., Vafiadi, C., & Dijkhuizen, L. (2016). The gram-negative bacterium Azotobacter chroococcum NCIMB 8003 employs a new glycoside hydrolase family 70 4,6-α-glucanotransferase enzyme (GtfD) to synthesize a reuteran like polymer from maltodextrins and starch. Biochimica et biophysica acta, 1860(6), 1224-1236. https://doi.org/10.1016/j.bbagen.2016.02.005

2015

Meng, X., Dobruchowska, J. M., Pijning, T., Gerwig, G. J., & Dijkhuizen, L. (2016). Synthesis of New Hyper-Branched α-Glucans from Sucrose by Lactobacillus reuteri 180 Glucansucrase Mutants. Journal of Agricultural and Food Chemistry, 64(2), 433-442. https://doi.org/10.1021/acs.jafc.5b05161
Paul, C. J., Leemhuis, H., Dobruchowska, J. M., Grey, C., Onnby, L., van Leeuwen, S. S., Dijkhuizen, L., & Karlsson, E. N. (2015). A GH57 4-alpha-glucanotransferase of hyperthermophilic origin with potential for alkyl glycoside production. Applied Microbiology and Biotechnology, 99(17), 7101-7113. https://doi.org/10.1007/s00253-015-6435-2
Bai, Y., van der Kaaij, R. M., Leemhuis, H., Pijning, T., van Leeuwen, S. S., Jin, Z., & Dijkhuizen, L. (2015). Biochemical characterization of Lactobacillus reuteri Glycoside Hydrolase family 70 GTFB type of 4,6-α-Glucanotransferase enzymes that synthesize soluble dietary starch fibers. Applied and environmental microbiology, 81(20), 7223-7232. https://doi.org/10.1128/AEM.01860-15
Meng, X., Pijning, T., Dobruchowska, J. M., Gerwig, G. J., & Dijkhuizen, L. (2015). Characterization of the functional roles of amino acid residues in acceptor binding subsite +1 in the active site of the glucansucrase GTF180 enzyme of Lactobacillus reuteri 180. The Journal of Biological Chemistry, 290(50), 30131-30141. https://doi.org/10.1074/jbc.M115.687558
van Munster, J. M., Dobruchowska, J. M., Veloo, R., Dijkhuizen, L., & van der Maarel, M. J. E. C. (2015). Characterization of the starvation-induced chitinase CfcA and α-1,3-glucanase AgnB of Aspergillus niger. Applied Microbiology and Biotechnology, 99, 2209-2223. https://doi.org/10.1007/s00253-014-6062-3
Bai, Y., van der Kaaij, R. M., Woortman, A. J. J., Jin, Z., & Dijkhuizen, L. (2015). Characterization of the 4,6-α-glucanotransferase GTFB enzyme of Lactobacillus reuteri 121 isolated from inclusion bodies. BMC Biotechnology, 15, [49]. https://doi.org/10.1186/s12896-015-0163-7
Valk, V., Eeuwema, W., Sarian, F. D., van der Kaaij, R. M., & Dijkhuizen, L. (2015). Degradation of granular starch by the bacterium Microbacterium aurum B8.A involves a novel modular α-amylase enzyme system with FNIII and CBM25 domains. Applied and environmental microbiology, 81(19), 6610-6620. https://doi.org/10.1128/AEM.01029-15
Lammerts van Bueren, A., Saraf, A., Martens, E. C., & Dijkhuizen, L. (2015). Differential metabolism of Exopolysaccharides from probiotic Lactobacilli by the human gut symbiont Bacteroides thetaiotaomicron. Applied and environmental microbiology, 81(12), 3973-3983. https://doi.org/10.1128/AEM.00149-15
Wilbrink, M. H., Ten Kate, G. A., Sanders, P., Gerwig, G. J., van Leeuwen, S. S., Sallomons, E., Klarenbeek, B., Hage, J. H., van Vuure, C. A., Dijkhuizen, L., & Kamerling, J. P. (2015). Enzymatic Decoration of Prebiotic Galacto-oligosaccharides (Vivinal® GOS) with Sialic Acid using Trypanosoma cruzi Trans-Sialidase and Two Bovine Sialoglycoconjugates as Donor Substrates. Journal of Agricultural and Food Chemistry, 63(25), 5976-5984. https://doi.org/10.1021/acs.jafc.5b01505
van Munster, J. M., Sanders, P., ten Kate, G. A., Dijkhuizen, L., & van der Maarel, M. J. E. C. (2015). Kinetic characterization of Aspergillus niger chitinase CfcI using a HPAEC-PAD method for native chitin oligosaccharides. Carbohydrate Research, 407, 73-78. https://doi.org/10.1016/j.carres.2015.01.014
Aalbers, F., Turkenburg, J. P., Davies, G. J., Dijkhuizen, L., & Lammerts van Bueren, A. (2015). Structural and Functional Characterization of a Novel Family GH115 4-O-Methyl-α-Glucuronidase with Specificity for Decorated Arabinogalactans. Journal of Molecular Biology, 427(24), 3935-3946. https://doi.org/10.1016/j.jmb.2015.07.006
Meng, X., Dobruchowska, J. M., Gerwig, G. J., Kamerling, J. P., & Dijkhuizen, L. (2015). Synthesis of oligo- and polysaccharides by Lactobacillus reuteri 121 reuteransucrase at high concentrations of sucrose. Carbohydrate Research, 414, 85-92. https://doi.org/10.1016/j.carres.2015.07.011
van Munster, J. M., Nitsche, B. M., Akeroyd, M., Dijkhuizen, L., van der Maarel, M. J. E. C., & Ram, A. F. J. (2015). Systems Approaches to Predict the Functions of Glycoside Hydrolases during the Life Cycle of Aspergillus niger Using Developmental Mutants ∆brlA and ∆flbA. PLoS ONE, 10(1), [e0116269]. https://doi.org/10.1371/journal.pone.0116269
Gangoiti, J., Pijning, T., & Dijkhuizen, L. (2015). The Exiguobacterium sibiricum 255-15 GtfC enzyme representing a novel glycoside hydrolase 70 subfamily of 4,6-α-glucanotransferase enzymes. Applied and environmental microbiology, 82(2), 756-766. [AEM.03420-15]. https://doi.org/10.1128/AEM.03420-15
Meng, X., Dobruchowska, J. M., Pijning, T., Gerwig, G. J., Kamerling, J. P., & Dijkhuizen, L. (2015). Truncation of domain V of the multidomain glucansucrase GTF180 of Lactobacillus reuteri 180 heavily impairs its polysaccharide-synthesizing ability. Applied Microbiology and Biotechnology, 99(14), 5885-5894. https://doi.org/10.1007/s00253-014-6361-8

2014

Venkataraman, H., Te Poele, E. M., Rosłoniec, K. Z., Vermeulen, N., Commandeur, J. N. M., van der Geize, R., & Dijkhuizen, L. (2015). Biosynthesis of a steroid metabolite by an engineered Rhodococcus erythropolis strain expressing a mutant cytochrome P450 BM3 enzyme. Applied Microbiology and Biotechnology, 99(11), 4713-4721. https://doi.org/10.1007/s00253-014-6281-7
Bultema, J. B., Kuipers, B. J. H., & Dijkhuizen, L. (2014). Biochemical characterization of mutants in the active site residues of the beta-galactosidase enzyme of Bacillus circulans ATCC 31382. FEBS Open Bio, 4(1), 1015-1020. https://doi.org/10.1016/j.fob.2014.11.002
van Leeuwen, S. S., Kuipers, B. J. H., Dijkhuizen, L., & Kamerling, J. P. (2014). Development of a H-1 NMR structural-reporter-group concept for the analysis of prebiotic galacto-oligosaccharides of the [beta-D-Galp-(1 -> x)](n)-D-Glcp type. Carbohydrate Research, 400, 54-58. https://doi.org/10.1016/j.carres.2014.08.011
Pijning, T., Vujicic - Zagar, A., Kralj, S., Dijkhuizen, L., & Dijkstra, B. W. (2014). Flexibility of truncated and full-length glucansucrase GTF180 enzymes from Lactobacillus reuteri 180. Febs Journal, 281(9), 2159-2171. https://doi.org/10.1111/febs.12769
Wilbrink, M. H., ten Kate, G. A., van Leeuwen, S. S., Sanders, P., Sallomons, E., Hage, J. A., Dijkhuizen, L., & Kamerling, J. P. (2014). Galactosyl-Lactose Sialylation Using Trypanosoma cruzi trans-Sialidase as the Biocatalyst and Bovine kappa-Casein-Derived Glycomacropeptide as the Donor Substrate. Applied and environmental microbiology, 80(19), 5984-5991. https://doi.org/10.1128/AEM.01465-14
Jonsson Wheat, J. O., Hreggvidsson, G. O., Fridjonsson, O. H., Dobruchowska, J. M., Kamerling, J. P., & Dijkhuizen, L. (2014). Glucan branching enzymes and their methods of use. (Patent No. Netherlands). https://nl.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2014155395A2&KC=A2&FT=D&ND=3&date=20141002&DB=&locale=nl_NL
Hill, P., Piel, J., Aris‑Brosou, S., Krištůfek, V., Boddy, C. N., & Dijkhuizen, L. (2014). Habitat‑specific type I polyketide synthases in soils and street sediments. Journal of Industrial Microbiology & Biotechnology, 41(1), 75-85. https://doi.org/10.1007/s10295-013-1362-7
van Leeuwen, S. S., Kuipers, B. J. H., Dijkhuizen, L., & Kamerling, J. P. (2014). H-1 NMR analysis of the lactose/beta-galactosidase-derived galacto-oligosaccharide components of Vivinal (R) GOS up to DP5. Carbohydrate Research, 400, 59-73. https://doi.org/10.1016/j.carres.2014.08.012
Leemhuis, H., Dobruchowska, J. M., Ebbelaar, M., Faber, F., Buwalda, P. L., van der Maarel, M. J. E. C., Kamerling, J. P., & Dijkhuizen, L. (2014). Isomalto/Malto-polysaccharide, a novel soluble dietary fiber made via enzymatic conversion of starch. Journal of Agricultural and Food Chemistry, 62(49), 12034-12044. https://doi.org/10.1021/jf503970a
van Leeuwen, S. S., Schoemaker, R. J. W., Gerwig, G. J., van Leusen-van Kan, E. J. M., Dijkhuizen, L., & Kamerling, J. P. (2014). Rapid milk group classification by 1H NMR analysis of Le and H epitopes in human milk oligosaccharide donor samples. Glycobiology, 24(8), 728-739. https://doi.org/10.1093/glycob/cwu036
Meng, X., Dobruchowska, J. M., Pijning, T., Lopez-Bautista, C., Kamerling, J. P., & Dijkhuizen, L. (2014). Residue Leu(940) Has a Crucial Role in the Linkage and Reaction Specificity of the Glucansucrase GTF180 of the Probiotic Bacterium Lactobacillus reuteri 180. The Journal of Biological Chemistry, 289(47), 32773-32782. https://doi.org/10.1074/jbc.M114.602524
Petrusma, M., van der Geize, R., & Dijkhuizen, L. (2014). 3-Ketosteroid 9 alpha-hydroxylase enzymes: Rieske non-heme monooxygenases essential for bacterial steroid degradation. Antonie Van Leeuwenhoek: International Journal of General and Molecular Microbiology, 106(1), 157-172. https://doi.org/10.1007/s10482-014-0188-2

2013

Timm, M., Goerl, J., Kraus, M., Kralj, S., Hellmuth, H., Beine, R., Buchholz, K., Dijkhuizen, L., Seibel, J., & Seibel, J. (2013). An Unconventional Glycosyl Transfer Reaction: Glucansucrase GTFA Functions as an Allosyltransferase Enzyme. ChemBioChem, 14(18), 2423-2426. https://doi.org/10.1002/cbic.201300392
Bokhove, M., Claessen, D., de Jong, W., Dijkhuizen, L., Boekema, E. J., & Oostergetel, G. T. (2013). Chaplins of Streptomyces coelicolor self-assemble into two distinct functional amyloids. Journal of Structural Biology, 184(2), 301-309. https://doi.org/10.1016/j.jsb.2013.08.013
van Munster, J. M., Nitsche, B. M., Krijgsheld, P., van Wijk, A., Dijkhuizen, L., Wösten, H. A., Ram, A. F., & van der Maarel, M. J. E. C. (2013). Chitinases CtcB and CfcI modify the cell wall in sporulating aerial mycelium of Aspergillus niger. Microbiology-Sgm, 159(9), 1853-1867. https://doi.org/10.1099/mic.0.067967-0
Leemhuis, H., Pijning, T., Dobruchowska, J. M., van Leeuwen, S. S., Kralj, S., Dijkstra, B. W., & Dijkhuizen, L. (2013). Glucansucrases: Three-dimensional structures, reactions, mechanism, alpha-glucan analysis and their implications in biotechnology and food applications. Journal of Biotechnology, 163(2), 250-272. https://doi.org/10.1016/j.jbiotec.2012.06.037
Dobruchowska, J. M., Meng, X., Leemhuis, H., Gerwig, G. J., Dijkhuizen, L., & Kamerling, J. P. (2013). Gluco-oligomers initially formed by the reuteransucrase enzyme of Lactobacillus reuteri 121 incubated with sucrose and malto-oligosaccharides. Glycobiology, 23(9), 1084-1096. https://doi.org/10.1093/glycob/cwt048
Puspasari, F., Radjasa, O. K., Noer, A. S., Nurachman, Z., Syah, Y. M., van der Maarel, M., Dijkhuizen, L., Janecek, S., Natalia, D., & Janeček, Š. (2013). Raw starch-degrading alpha-amylase from Bacillus aquimaris MKSC 6.2: isolation and expression of the gene, bioinformatics and biochemical characterization of the recombinant enzyme. Journal of Applied Microbiology, 114(1), 108-120. https://doi.org/10.1111/jam.12025
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