Publication

Protein Profile Analysis of Two Australian Snake Venoms by OneDimensional Gel Electrophoresis and MS/MS Experiments

Georgieva, D., Hildebrand, D., Simas, R., Coronado, M. A., Kwiatkowski, M., Schlueter, H., Arni, R., Spencer, P. & Betzel, C. 2017 In : CURRENT MEDICINAL CHEMISTRY. 24, 17, p. 1892-1908 17 p.

Research output: Scientific - peer-reviewArticle

  • Dessislava Georgieva
  • Diana Hildebrand
  • Rodrigo Simas
  • Monika A. Coronado
  • Marcel Kwiatkowski
  • Hartmut Schlueter
  • Raghuvir Arni
  • Patrick Spencer
  • Christian Betzel

The Pseudechis colletti and Pseudechis butleri venoms were analyzed by 1-D gel electrophoresis, followed by mass spectrometric analysis of tryptic peptides obtained from the protein bands. Both venoms contain highly potent pharmacologically active components, which were assigned to the following protein families: basic and acidic phospholipases A(2) (PLA(2)s), L-amino acid oxidases (LAAOs), P-III metalloproteinases (P-III SVMPs), 5'nucleotidases (5'-NTDs), cysteine-rich secretory proteins (CRISPs), venom nerve growth factors (VNGFs) and post-synaptic neurotoxins. Considerable predominance of PLA(2)s over other toxins is a characteristic feature of both venoms. The major differences in the venom compositions are the higher concentration of SVMPs and CRISPs in the P. butleri venom, as well as the presence of post-synaptic neurotoxins. Furthermore, the analysis revealed a high concentration of proteins with myotoxic, coagulopathic and apoptotic activities. PLA(2)s are responsible for the myotoxic and anticoagulant effects observed in patients after envenomation (4). The other protein families, encountered in the two venoms, probably contribute to the major symptoms described for these venoms. These results explain the observed clinical effects of the black snake envenomation. The analyzed venoms contain group P-III metalloproteinases of medical importance with the potency to be used for diagnostic purposes of von Willebrand factor (vWF) disease, for regulation of vWF in thrombosis and haemostasis, for studying the function of the complement system in host defense and in the pathogenesis of diseases. Comparison of venomic data showed similarities in the major venom components of snakes from the genus Pseudechis, resulting in common clinical effects of envenomation, and demonstrating close relationships between venom toxins of Elapidae snakes.

Original languageEnglish
Pages (from-to)1892-1908
Number of pages17
JournalCURRENT MEDICINAL CHEMISTRY
Volume24
Issue number17
StatePublished - 2017
Externally publishedYes

    Keywords

  • Proteome, Snake venom, Pseudechis butleri, Pseudechis colletti, protein profile analysis., PSEUDECHIS SPP. VENOMS, PHYLOGENETIC ANALYSIS, TOXIN SEQUENCES, COLLETTS SNAKE, BLACK, METALLOPROTEINASE, EVOLUTION, ANTIVENOMS, EFFICACY, NEUROTOXIN

View graph of relations

ID: 48655453