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Phylogeographic differentiation versus transcriptomic adaptation to warm temperatures in Zostera marina, a globally important seagrass

Jueterbock, A., Franssen, S. U., Bergmann, N., Gu, J., Coyer, J. A., Reusch, T. B. H., Bornberg-Bauer, E. & Olsen, J. L. 22-Nov-2016 In : Molecular Ecology. 25, 21, p. 5396-5411 16 p.

Research output: Scientific - peer-reviewArticle

  • Alexander Jueterbock
  • S. U. Franssen
  • N. Bergmann
  • J. Gu
  • J. A. Coyer
  • T. B. H. Reusch
  • E. Bornberg-Bauer
  • J. L. Olsen

Populations distributed across a broad thermal cline are instrumental in addressing adaptation to increasing temperatures under global warming. Using a space-for-time substitution design, we tested for parallel adaptation to warm temperatures along two independent thermal clines in Zostera marina, the most widely distributed seagrass in the temperate Northern Hemisphere. A North-South pair of populations was sampled along the European and North American coasts and exposed to a simulated heatwave in a common-garden mesocosm. Transcriptomic responses under control, heat stress and recovery were recorded in 99 RNAseq libraries with similar to 13 000 uniquely annotated, expressed genes. We corrected for phylogenetic differentiation among populations to discriminate neutral from adaptive differentiation. The two southern populations recovered faster from heat stress and showed parallel transcriptomic differentiation, as compared with northern populations. Among 2389 differentially expressed genes, 21 exceeded neutral expectations and were likely involved in parallel adaptation to warm temperatures. However, the strongest differentiation following phylogenetic correction was between the three Atlantic populations and the Mediterranean population with 128 of 4711 differentially expressed genes exceeding neutral expectations. Although adaptation to warm temperatures is expected to reduce sensitivity to heatwaves, the continued resistance of seagrass to further anthropogenic stresses may be impaired by heat-induced downregulation of genes related to photosynthesis, pathogen defence and stress tolerance.

Original languageEnglish
Pages (from-to)5396-5411
Number of pages16
JournalMolecular Ecology
Volume25
Issue number21
StatePublished - 22-Nov-2016

    Keywords

  • common-garden experiment, differential expression, global warming, heatwave, RNAseq, transcriptomics, CLIMATE-CHANGE, R-PACKAGE, PHENOTYPIC PLASTICITY, LABYRINTHULA-ZOSTERAE, QUANTITATIVE TRAITS, GENE-EXPRESSION, WASTING DISEASE, Z-NOLTII, STRESS, POPULATIONS

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