Multidrug resistance regulators (MDRs) as scaffolds for the design of artificial metalloenzymesBersellini, M. & Roelfes, G. 14-Apr-2017 In : Organic & Biomolecular Chemistry. 15, 14, p. 3069-3073 5 p.
Research output: Scientific - peer-review › Article
The choice of protein scaffolds is an important element in the design of artificial metalloenzymes. Herein, we introduce Multidrug Resistance Regulators (MDRs) from the TetR family as a viable class of protein scaffolds for artificial metalloenzyme design. In vivo incorporation of the metal binding amino acid (2,2-bipyridin- 5yl) alanine (BpyA) by stop codon suppression methods was used to create artificial metalloenzymes from three members of the TetR family of MDRs: QacR, CgmR and RamR. Excellent results were achieved with QacR Y123BpyA in the Cu2+ catalyzed enantioselective vinylogous Friedel-Crafts alkylation reaction with ee's up to 94% of the opposite enantiomer that was achieved with other mutants and the previously reported LmrR-based artificial metalloenzymes.
|Number of pages||5|
|Journal||Organic & Biomolecular Chemistry|
|State||Published - 14-Apr-2017|
- BIS(OXAZOLINYL)PYRIDINE-SCANDIUM(III) TRIFLATE COMPLEXES, FRIEDEL-CRAFTS ALKYLATIONS, UNNATURAL AMINO-ACIDS, TRANSCRIPTIONAL REPRESSOR, METAL-BINDING, GENETIC INCORPORATION, PROTEIN, LMRR, QACR, RECOGNITION