In Vivo Imaging Analysis

 

In Vivo Optical Imaging is a non-destructive technique which enables direct monitoring of luminescent or fluorescent cells and microbes in vivo (both in animal models and humans). This technique has recently been successfully applied in oncological studies with luminescent cell lines that would otherwise require histopathology to identify, as well as in bio-adhesion studies with fluorescently labeled and luminescent bacteria which study the development of bacterial bio-layers that cause biomaterial associated infections. The course will evaluate the optical technique, stressing the quantification of the optical IVIS image. To this end, relevant optical principles will be discussed such as the origin and character of bioluminescence and fluorescence, scattering and absorption in tissue affecting the intensity of the image, the theory of radiance and the first principles of optical 3D tomography enabling the exact location of the light emitting source inside the body, spectral unmixing, image acquisition and image processing. A number of fluorescent probes will be discussed in relation to the cell metabolism with which they interfere. The several advantages of the system for diverse applications as well as on the pitfalls and concerns to deal with while applying the technique will also be dealt with.
 

Study load

0.25 ECTS credit point

 

Calendar

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Registration

J. (Han) Sjollema, PhD (j.sjollema@med.umcg.nl)

Department of Biomedical Engineering, W.J. Kolff Institute

Phone: +31 50 363 3149

 

Coordinator
J. (Han) Sjollema, PhD

Department of Biomedical Engineering, W.J. Kolff Institute

 

Additional information

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