Dr. Peter Horvatovich

Antonius Deusinglaan 1
Postbus 196
9700 AD Groningen
The Netherlands

Room: (3211) 258
Tel: +31 50 3633341
Fax: +31 50 3637582
E-Mail: p.l.horvatovich(at)rug.nl

 

Introduction

I have graduated at the University of Ötvös Lóránd in Budapest preparing my diploma thesis in the field of environmental analytical chemistry by analyzing bakelits using supercritical fluid extraction. After finishing 5 year study at this University I obtained a DEA diploma in Physical-Chemistry at the University of Louis Pasteur in Strasbourg. I have realized then a PhD between the University of Louis Pasteur in Strasbourg and University of Horticulture in Budapest in food analytical chemistry in the field of the detection of irradiated food by developing a sensitive detection method for 2-alkylcyclobutanones, marker produced specifically from triglyceride by ionization. After my defense in March 2001, I worked at Sanofi-Synthelabo in Budapest as molecular modeler and manager of the local chemical library. After March 2003, I obtained a Scholarship of Alexander von Humboldt and Hertie foundation and within the subprogram Roman Herzog I got a postdoctoral experience in the Federal Institute for Risk Assessment in Proteomics where I have participated in the development of analytical methods for the detection of illegal use of Recombinant Bovine Somatotropine in lactating cattle. From January 2005, I am working at the University of Groningen at the Department of Analytical Biochemistry in biomarker discovery for cervical cancer.  

Current research

I am developing bioinformatics tools for processing and statistical analysis of LC-MS data obtained for biomarker discovery in Matlab, C++ and java. My main work consists of developing a comprehensive data processing methodology including signal processing using 2 dimensional smoothing, peak picking, time alignment, normalization and peak matching of multiple chromatograms of online and off-line coupled LC-MS data for different types of LC (e.g. capillary, nano-, chip-HPLC) and mass analyzer (e.g. quadrupole, iontrap, TOF, FTMS). The processed data is then submitted to statistical analysis using supervised classification method (e.g. Nearest Shrunken Centroids) and finally the results are statistically, analytically and biologically validated. This work is realized with strong collaboration of numerous international partners, e.g. IBM Watson Center (Frank Suits), TNO (Renger Jellema), University of Leiden (Theo Reijmers) and within the Biorange project with University of Amsterdam (Age Smilde). I participate in the developing and testing of different analytical platforms, fractionation methods and selective concentration of class of compounds using different affinity columns for label free profiling study for biomarker discovery purpose. I am closely involved in biomarker discovery for early stage cervical cancer using preclassified serum samples (see more detailed descriptions can be found at the home page of Natalia Govorukhina.

 

Publications: 13. 

Selected references

Horvatovich P. , Werner D., Jung S., Miesch M., Delincee H., Hasselmann C., Marchioni E., Determination of 2-Alkylcyclobutanones with Electron Impact and Chemical Ionization GC-MS in irradiated foods, J. Agric. Food Chem., 54 (2006) 1990-1996.

Horvatovich P. , Miesch M., Hasselmann C., Delincee H., Marchioni E., Determination of monounsaturated alkyl side chain 2-alkylcyclobutanones in irradiated foods. J. Agric. Food Chem.,53 (2005) 5836- 58416.

Horvatovich P., Raul F., Miesch M., Burnouf D., Delincée H., Hartwig A., Werner D., Marchioni E., Detection of 2-Alkylcyclobutanones, markers for irradiated foods, in adipose tissues of animals fed with these substances, J. Food Protect, 65(10) (2002) 1610-1613.

Horvatovich P., Miesch M., Hasselmann C., Marchioni E., Supercritical fluid extraction of hydrocarbons and 2‑alkylcyclobutanones for the detection of ir­ra­di­at­ed foodstuffs, Journal of Chro­ma­tography A, 897 (2000) 259-268.