Prof.Dr. Rainer Bischoff

Antonius Deusinglaan 1
Postbus 196
9700 AD Groningen
The Netherlands

Room: (3211) 262
Tel: +31 50 3633338
Fax: +31 50 3637582
E-Mail: r.p.h.bischoff(at)rug.nl

Introduction

Prof. Bischoff received his PhD in chemistry from the University of Göttingen (Germany) with a work on novel stationary phases for the separation of transfer RNAs by HPLC. The work was performed at the Max-Planck-Institute for Experimental Medicine. After a 2-year postdoctoral period in the laboratory of Prof. Fred E. Regnier (Purdue University, West-Lafayette, USA) focusing on the development of affinity separation matrices for nucleic acids, he started as a research scientist at the biotechnology company Transgene S.A. (Strasbourg, France). In his function as group and project leader Bischoff worked on the production and analysis of recombinant proteins for pharmaceutical applications, the discovery of novel proteins involved in infectious disease as well as on the development of novel gene transfer vectors based on non-viral vehicles. Prof. Bischoff's career continued as assistant director and section manager at AstraZeneca R&D in Lund (Sweden), where he signed responsible for protein chemistry and high-throughput assay development.

Research

Title

Analysis of Biomacromolecules

Description

Prof. Bischoff was appointed to the chair in Analysis of Biomacromolecules in January 2001. His research interests focus on Targeted Proteomics based on selective sample enrichment techniques such as chemical ligands (e.g. protease inhibitors), biological ligands (e.g. antibodies) and macromolecular chemical structures (e.g. polymers and molecular imprints). The group uses state-of-the-art equipemnt for protein and peptide separation and identification mainly based on high-performance liquid chromatography coupled to mass spectrometry.

Key References

Bischoff, R, Speck D, Lepage P, Delatre L, Ledoux C, Brown SW et al. Purification and biochemical characterization of recombinant alpha 1-antitrypsin variants expressed in Escherichia coli. Biochemistry 1991; 30(14):3464-3472.

Bischoff, R, Lepage P, Jaquinod M, Cauet G, Acker-Klein M, Clesse D et al. Sequence-specific deamidation: isolation and biochemical characterization of succinimide intermediates of recombinant hirudin. Biochemistry 1993; 32(2):725-734.

Klarskov K, Roecklin D, Bouchon B, Sabatie J, Van Dorsselaer A, Bischoff, R. Analysis of recombinant Schistosoma mansoni antigen rSmp28 by on-line liquid chromatography-mass spectrometry combined with sodium dodecyl sulfate polyacrylamide gel electrophoresis. Anal Biochem 1994; 216(1):127-134

Roecklin D, Klarskov K, Cavallini B, Sabatie J, Bouchon B, Loew D et al. Addition of acetaldehyde to the N-terminus of a recombinant Schistosoma mansoni glutathione S-transferase upon high-level expression in Saccharomyces cerevisiae. Eur J Biochem 1997; 245(3):589-599.

Menozzi FD, Bischoff, R, Fort E, Brennan MJ, Locht C. Molecular characterization of the mycobacterial heparin-binding hemagglutinin, a mycobacterial adhesin. Proc. Natl. Acad. Scienc. USA 1998; 95(21):12625-12630.

Wagner K, Racaityte K, Unger K.K., Miliotis T, Edholm LE, Bischoff, Ret al. Protein mapping by two-dimensional high performance liquid chromatography.Journal of Chromatography, 893, 293-305 (2000).

Miliotis T, Ericsson PO, Marko-Varga G, Svensson R, Nilsson J, Laurell T et al. Analysis of regulatory phosphorylation sites in ZAP-70 by capillary high-performance liquid chromatography coupled to electrospray ionization or matrix-assisted laser desorption ionization time-of-flight mass spectrometry. J. Chromatogr. B 2001; 752(2):323-334.

Wagner K, Miliotis T, Marko-Varga G, Bischoff, R, Unger KK. An automated on-line multidimensional HPLC system for protein and peptide mapping with integrated sample preparation. Anal. Chem. 2002; 74(4):809-820.

Bischoff, R. Characterization of Proteins, Peptides, and Polynucleotides by Mass Spectrometry. In: Gooding KM, Regnier F, editors. HPLC of Biological Macromolecules. New York: Marcel Dekker, Inc., 2002: 689-738.

Links

Groningen University Institute for Drug Exploration (GUIDE)