10 Commandments of VMT

 

1. All VMT (veilige microbiologische technieken, English: safe microbiological techniques) related work can only be performed by those people that have permission from the Biological Safety Officer (BVF; Onno de Vries). Work according to the rules, even if you believe there is no apparent risk.

 

2. During VMT related work all doors and windows have to be closed. Verify that insects and other pests are not present in the lab.

 

3. Wear a closed labcoat. Do not take this labcoat outside the VMT area unless it is absolutely necessary for the experiment. In case of a contamination of the labcoat, sterilize the labcoat first, with bleach or autoclaving, before washing.

 

4. Clean and sterilize spills immediately. Repost serious contamination immediately to the BVF.

 

5. It is absolutely prohibited to eat, drink or smoke, or to have cups, plates, mugs or silverware in the VMT area.

 

6. Pipetting by mouth is prohibited. Used pipets are collected in a disinfecting solution.

 

7. Prevent aerosols. These may be created by - splashing drops; - pouring of liquids; - discharging pipettes; - opening of moist plugs; - using inoculating loops that are too hot. Use needles only if there is absolutely no alternative.

 

8. Glassware and instruments that have been in contact with GMO's (Genetically Modified Organisms, GGO's in English) have to be sterilized or desinfected before being washed, reused or discarded. Biological waste has to be collected in autoclavable plastic bags, which are autoclaved before discarding (use indicator tape to demonstrate that the bag was autoclaved).

 

9. Wash hands with soap and water after work and before leaving the room. Bench surface areas have to be cleaned and desinfected daily. Kep area clean and organized.

 

10. Record the general nature of the work clearly in a labjournal

Links

Ministry of VROM

RIVM

BVF-platform